CEDIA, a new homogeneous immunoassay system.

Henderson, Daniel R.; Friedman, Stanford B.; Harris, Jeffrey D.; Manning, Wayne B.; Zoccoli, M A

doi:10.1093/clinchem/32.9.1637

Cited by 138 publications

(27 citation statements)

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“…The reagents were mixed and incubated at 37°C, and the rate of hydrolysis was measured at 450 nm. The concentration of total T 4 in the patient specimens and controls were determined using a linear calibration curve (16). FTI was calculated from a T 3 uptake assay in which 125 I-labeled T 3 was used as the tracer in the T 3 uptake assay to fill the unbound thyroxine-binding globulin (TBG) sites.…”

Section: Hormone Analysismentioning

confidence: 99%

The effects of PCB exposure and fish consumption on endogenous hormones.

Persky

Turyk

Anderson

et al. 2001

Environ Health Perspect

157

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Previous studies have suggested that exposure to polychlorinated biphenyls (PCBs) may alter thyroid function, but data on effects of PCB exposure on other endogenous hormones has been lacking. The current study is ancillary to a larger investigation of the effects of Great Lakes fish consumption on PCBs and reproductive function. In the current study we examine associations of PCBs, 1,1-bis (4-chlorophenyl)-2,2-dichloroethene (DDE), and fish consumption with thyroid and steroid hormones in 178 men and PCBs, DDE, and fish consumption with thyroid hormones in 51 women from the original study. Serum PCB level and consumption of Great Lakes fish are associated with significantly lower levels of thyroxine (T(4)) and free thyroxine index (FTI) in women and with significantly lower levels of T(4) in men. Fish consumption, but not PCB level, is significantly and inversely associated with triiodothyronine (T(3)) in men. Results for thyroid-stimulating hormone (TSH) are inconsistent. Among men, there are significant inverse associations of both PCB and fish consumption with sex hormone-binding globulin (SHBG)-bound testosterone, but no association with SHBG or free testosterone. There are no significant overall associations of PCB, DDE, or fish consumption with estrone sulfate, follicle-stimulating hormone, luteinizing hormone, or dehydroepiandrosterone sulfate. The results of this study are consistent with previous studies showing effects of fish consumption and PCB exposure on thyroid hormones and suggest that PCBs may also decrease steroid binding to SHBG. Elucidation of specific mechanisms must await future investigations.

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Section: Hormone Analysismentioning

confidence: 99%

The effects of PCB exposure and fish consumption on endogenous hormones.

Persky

Turyk

Anderson

et al. 2001

Environ Health Perspect

157

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“…b Concentration of chemicals that increase umuC gene expression by twofold over background levels extrapolated from doses resulting in increasing responses in Tables I and II. interpretation (see Results), CPRG appears to be more useful than ONPG as a substrate for ␤-galactosidase activity in our assay system. CPRG is also used instead of ONPG in an immunoassay system [Henderson et al, 1986], in an assay to detect ␤-galactosidase in crude homogenates from germline-transfected Drosophila [Simon and Lis, 1987], as well as in a liquid colorimetric presence/absence coliphage detection method [Ijzerman et al, 1993]. CRPG was reported to be 10 times more sensitive than ONPG in kinetic assays of ␤-galactosidase in transfected HeLa cells [Eustice et al, 1991].…”

Section: Discussionmentioning

confidence: 99%

Use of a high‐throughput umu‐microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter

Oda

Funasaka

Kitano

et al. 2004

Environ and Mol Mutagen

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In the present study, we developed a rapid umu-microplate test system that uses the nitroreductase- and O-acetyltransferase-overproducing Salmonella typhimurium strain NM3009 and the O-acetyltransferase-overproducing S. typhimurium strain NM2009 to detect genotoxic activity in small volume samples. The assay was used to test the genotoxicity of several standard mutagens and environmental samples. Exponentially growing cultures of NM3009, NM2009, and the parental strain TA1535/pSK1002 were incubated in 96-well microplates with test chemicals both in the presence and in the absence of rat liver S9. The relative beta-galactosidase activities were then determined colorimetrically using either chlorophenol red-beta-D-galactopyranoside (CPRG) or O-nitrophenyl-beta-D-galactopyranoside (ONPG) as a measure of umuC gene induction activity. The sensitivities of NM3009 without S9 mix and NM2009 with S9 mix to nitroarenes and aromatic amines were up to 24- to 75-fold higher than those of the parent strain. Induction of umuC gene expression was detected more readily with CPRG than ONPG. The umu-microplate assay also detected genotoxicity in organic extracts of particulate matter from air samples collected in Osaka City, Japan. The pattern of the responses suggested that the genotoxic activity of the particulate extract was due primarily to nitrated polycyclic aromatic hydrocarbons. Our results indicate that the umu-microplate assay may be a useful way of carrying out rapid screens for genotoxicity in small-volume environmental samples.

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“…After equilibration, the cells were treated with 0-100 nM of 5-HT and with or without 1 lM forskolin for 30 min at 37°C. Intracellular cyclic AMP was measured by the enzyme fragment complemention (EFC) method (Henderson et al 1986) with anti-cyclic AMP antibody using a HitHunterÔ EFC Cyclic AMP Chemiluminescence Assay Kit (Applied Biosystems, Fremont, CA, USA). Experimental procedures followed an adherent cell-specialized protocol provided by the manufacturer (Applied Biosystems).…”

Section: Measurement Of Cyclic Amp Amount In C6 Glioma Cellsmentioning

confidence: 99%

Recombinant human serotonin 5A receptors stably expressed in C6 glioma cells couple to multiple signal transduction pathways

Нода

Yasuda

Okada

et al. 2003

Journal of Neurochemistry

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Human serotonin 5A (5-HT 5A ) receptors were stably expressed in undifferentiated C6 glioma. In 5-HT 5A receptors-expressing cells, accumulation of cAMP by forskolin was inhibited by 5-HT as reported previously. Pertussis toxin-sensitive inhibition of ADP-ribosyl cyclase was also observed, indicating a decrease of cyclic ADP ribose, a potential intracellular second messenger mediating ryanodine-sensitive Ca 2+ mobilization. On the other hand, 5-HT-induced outward currents were observed using the patch-clamp technique in whole-cell configuration. The 5-HT-induced outward current was observed in 84% of the patched 5-HT 5A receptor-expressing cells and was concentration-dependent. The 5-HT-induced current was inhibited when intracellular K + was replaced with Cs + but was not significantly inhibited by typical K + channel blockers. The 5-HT-induced current was significantly attenuated by 1,2-bis(2-aminophenoxy)ethane-N,N,N¢,N¢-tetraacetic acid (BAPTA) in the patch pipette. Depleting intracellular Ca 2+ stores by application of caffeine or thapsigargin also blocked the 5-HT-induced current. Blocking G protein, the inositol triphosphate (IP3) receptor, or pretreatment with pertussis toxin, all inhibited the 5-HT-induced current. IP3 showed a transient increase after application of 5-HT in 5-HT 5A receptor-expressing cells. It was concluded that in addition to the inhibition of cAMP accumulation and ADP-ribosyl cyclase activity, 5-HT 5A receptors regulate intracellular Ca 2+ mobilization which is probably a result of the IP3-sensitive Ca 2+ store. These multiple signal transduction systems may induce complex changes in the serotonergic system in brain function. Keywords: adenylyl cyclase, ADP ribosyl cyclase, cAMP, IP3, 5-HT 5A receptors, patch-clamp. The neurotransmitter serotonin (5-HT) is involved in the control of diverse physiological processes including sleep, sexual behavior, food intake, locomotion and mood. At least 13 different 5-HT receptors have been identified to date. The present work concerns one of these, the 5-HT 5 receptor, which belongs to the superfamily of G protein-coupled metabotropic receptors.The two members of the 5-HT 5 receptor subfamily, 5-HT 5A and 5-HT 5B , were identified in mice (Plassat et al. 1992;Matthes et al. 1993) and subsequently in rats (Erlander et al. 1993;Wisden et al. 1993). A cDNA encoding the 5-HT 5A receptor has been cloned from human tissues, while the 5-HT 5B receptor does not seem to be functionally expressed

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CEDIA, a new homogeneous immunoassay system.

Cited by 138 publications

References 0 publications

The effects of PCB exposure and fish consumption on endogenous hormones.

The effects of PCB exposure and fish consumption on endogenous hormones.

Use of a high‐throughput umu‐microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter

Recombinant human serotonin 5A receptors stably expressed in C6 glioma cells couple to multiple signal transduction pathways

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