Use of a high‐throughput <i>umu</i>‐microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter

Oda, Yoshimitsu; Funasaka, Kunihiro; Kitano, Masaaki; Nakama, Akihiko; Yoshikura, Taro

doi:10.1002/em.10209

Cited by 46 publications

(33 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SOS/umu test is to detect the SOS responses by measuring the induction of umuC gene expression in a series of constructed S. typhimurium strains (TA1535/pSK1002 etc.) after treatment to DNA-damage agents (Oda et al, 2004). Multiple damages to DNA indicated by the SOS response can be detected using the SOS/umu test with S. typhimurium strain TA1535/ pSK1002 (Reifferscheid and Heil, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…S. typhimurium strain NM3009 can overproduce O-AT and NR, which are suggested to catalyze the key steps in the mutagenic activation of nitroarenes (Oda et al, 1993). If NM3009 have more sensitive response than TA1535/pSK1002 in detecting the genotoxic activities of environmental sample in the absence of S9, it would be concluded that the genotoxic activity is at least partially due to the nitroarenes (Oda et al, 2004). NM2009 overproducing O-AT has a high response as NM3009 to a few nitroarenes, such as 1,8-dinitropyrene, 3,7-dinitrofluoranthene, which may be activated to mutagens mainly by O-AT rather than NR (Oda et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The SOS/umu assay was performed according to Oda et al (2004) and Yan et al (2014b). Strain TA1535/pSK1002 was grown overnight in L-broth that contained 50 μg/mL of ampicillin at 37°C.…”

Section: Sos/umu Assaymentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action

Han

Oda

et al. 2016

Ecotoxicology and Environmental Safety

Self Cite

View full text Add to dashboard Cite

a b s t r a c tWith the burgeoning contamination of surface waters threatening human health, the genotoxic effects of surface waters have received much attention. Because mutagenic and carcinogenic compounds in water cause tumors by different mechanisms, a battery of bioassays that each indicate a different mode of action (MOA) is required to evaluate the genotoxic effects of contaminants in water samples. In this study, 15 water samples from two source water reservoirs and surrounding rivers in Shijiazhuang city of China were evaluated for genotoxic effects. Target chemical analyses of 14 genotoxic pollutants were performed according to the Environmental quality standards for surface water of China. Then, the in vitro cytokinesis-block micronucleus (CBMN) assay, based on a high-content screening technique, was used to detect the effect of chromosome damage. The SOS/umu test using strain TA1535/pSK1002 was used to detect effects on SOS repair of gene expression. Additionally, two other strains, NM2009 and NM3009, which are highly sensitive to aromatic amines and nitroarenes, respectively, were used in the SOS/umu test to avoid false negative results. In the water samples, only two of the genotoxic chemicals listed in the water standards were detected in a few samples, with concentrations that were below water quality standards. However, positive results for the CBMN assay were observed in two river samples, and positive results for the induction of umuC gene expression in TA1535/pSK1002 were observed in seven river samples. Moreover, positive results were observed for NM2009 with S9 and NM3009 without S9 in some samples that had negative results using the strain TA1535/pSK1002. Based on the results with NM2009 and NM3009, some unknown or undetected aromatic amines and nitroarenes were likely in the source water reservoirs and the surrounding rivers. Furthermore, these compounds were most likely the causative pollutants for the genotoxic effect of these water samples. Therefore, to identify causative pollutants with harmful biological effects, chemical analyses for the pollutants listed in water quality standards is not sufficient, and single-endpoint bioassays may underestimate adverse effects. Thus, a battery of bioassays based on different MOAs is required for the comprehensive detection of harmful biological effects. In conclusion, for genotoxicity screening of surface waters, the SOS/umu test system by using different strains combined with the CBMN assay was a useful approach.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action

Han

Oda

et al. 2016

Ecotoxicology and Environmental Safety

Self Cite

View full text Add to dashboard Cite

show abstract

“…The umu/SOS assays were carried out according to the procedure described previously by Oda et al [41], with some modifications.…”

Section: The Umu/sos Assaymentioning

confidence: 99%

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Fang

Ying

Zhao

et al. 2012

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Wastewaters from various industries are a main source of the contaminants in aquatic environments. The authors evaluated the hormonal activities (estrogenic/anti‐estrogenic activities, androgenic/anti‐androgenic activities) and genotoxicity of various effluents from textile and dyeing plants, electronic and electroplate factories, pulp and paper mills, fine chemical factories, and municipal wastewater treatment plants in the Pearl River Delta region by using in vitro bioassays (yeast estrogen screen [YES]; yeast androgen screen [YAS]; and genotoxicity assay [umu/SOS]) combined with chemical analysis. The results demonstrated the presence of estrogenic, anti‐estrogenic, and anti‐androgenic activity in most industrial effluents, whereas no androgenic activities were detected in all of the effluents. The measured estrogenic activities expressed as estradiol equivalent concentrations (EEQs) ranged from below detection (3 of 26 samples) to 40.7 ng/L, with a mean of 7.33 ng/L in all effluents. A good linear relationship was found between the EEQs measured by YES bioassay and the EEQs calculated from chemical concentrations. These detected estrogenic compounds, such as 4‐nonylphenol and estrone, were responsible for the estrogenic activities in the effluents. The genotoxic effects expressed as benzo[a]pyrene equivalent concentrations (BaP EQs) varied between below detection and 88.2 µg/L, with a mean of 8.76 µg/L in all effluents. The target polycyclic aromatic hydrocarbons were minor contributors to the genotoxicity in the effluents, and some nontarget compounds in the effluents were responsible for the measured genotoxicity. In terms of estrogenic activities and genotoxicity, discharge of these effluents could pose high risks to aquatic organisms in the receiving environments. Environ. Toxicol. Chem. 2012;31:1273–1282. © 2012 SETAC

show abstract

“…typhimurium NM1011 that overexpresses NR and S. typhimurium NM2009 that overexpresses O-AT strains were kindly provided by Dr. Y. Oda. The tester strains were constructed by introducing a vector plasmid pACY184 carrying only the NR gene (NM1011) or only the O-AT gene (NM2009) into the parent strain S. typhimurium TA1535/ pSK1002 harboring the umuC'-'lacZ fusion gene [14]. …”

mentioning

confidence: 99%

Genotoxicity of potent antiviral 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives designed as drug agents

Doğan¹,

Durusoy²,

Gökçe³

et al. 2014

Turk J Bioch

View full text Add to dashboard Cite

Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umu-microplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-D-galactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn't caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate. Key Words: Nitro compounds, umu-microplate, genotoxicity, drug Conflict of Interest: Authors have no conflict of interest. ÖZETAmaç: Bu çalışmada, ilaç etken maddesi olarak tasarlanmış altı adet nitrobütan türevinin genotoksik potansiyelleri, umu-mikroplak test sistemi ile değerlendirilmiştir. İn vitro testlerde anlamlı bir ilaç aktivitesinin saptanması durumunda, güvenlik testlerinin uygulanması, ilaç geliştirmede çok önemli bir ilkedir. Kemoterapide hastayı risk oluşturmadan tedavi etmek esas olduğundan, güvenlik testleri ilacın deneysel koşullardaki etkinliğinden bile daha fazla önem taşıyabilir. Metod: Umu-mikroplak test sistemi özel olarak nitrolu bileşiklerin mutajenitesini saptamak için tasarlanmıştır. 1-[(2-aminofenil)tiyo]-1-fenil-2-nitrobütan (I) türevleri de nitro grubu içermektedir, bu nedenle çalışmamızda bu test sistemi seçilmiştir. SOS indükleme aktivitelerinin değerlendirildiği umu-mikroplak test sisteminde, nitrolu bileşiklere hassas olarak geliştirilmiş, NR (nitroredüktaz) enzimini normalden fazla ifade e...

show abstract

Use of a high‐throughput umu‐microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter

Cited by 46 publications

References 40 publications

Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action

Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Genotoxicity of potent antiviral 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives designed as drug agents

Contact Info

Product

Resources

About