Cecropia obtusa extract and chlorogenic acid exhibit anti aging effect in human fibroblasts and keratinocytes cells exposed to UV radiation

Abstract

Cecropia obtusa is popularly used in the Amazonian region and exhibits antioxidant activity. Cosmetic formulations containing C . obtusa extract are commercially available for purchase; however, the chemical composition and the effects of the topical application of the extract are not described in the literature. Therefore, this study aimed to identify the main components of C . obtusa for the first time and to assess the anti aging effect in human fibroblasts and keratinocytes exposed to UVR. The main components in C . obtusa extract were identified by LC-DAD-MS/MS as chlorogenic acid (CGA), luteolin-C-hexoside, luteolin-C-hexose-O-deoxy-hexose, and apigenin-C-hexose-O-deoxy-hexose. C . obtusa extract and CGA decreased the metalloproteinase-1 and protein carbonyl levels and increased the collagen and hyaluronic acid contents. Overall, the extract exhibited better activity than CGA, and we demonstrated the ability of the extract to protect against the UV-induced increase in the pro inflammatory cytokines IL-1β and IL-6, which are potential pathways of the antioxidant and anti aging effect. The chemical characterization added important data to broaden the knowledge related to C . obtusa , and the results suggest that the extract is a promising candidate to be incorporated in topical photochemoprotective formulations.

Almond Skin Extracts and Chlorogenic Acid Delay Replicative Aging by Enhanced Oxidative Stress Response Involving SIR2 and SOD2 in Yeast

“…This suggests that AE and CHL could reduce the carbonyl protein content both by scavenging ROS / RNS as well as by decreasing the lipid peroxidation process. In line with our observation, CHL has been proposed to reduce the protein carbonyl content in human fibroblasts and keratinocytes cells exposed to UV radiation [50].…”

“…Protein carbonyls can form covalent adducts with cellular components that can result in structural alterations and alter their function. Although ROS and RNS are highly reactive and cause site-specific injury, protein carbonyls are more stable and may spread to distant sites, increasing oxidative damage [50]. The protection offered by AE and CHL against UV-C-induced protein (Figure 5c).…”

“…The content of carbonyl protein was clearly reduced by returning to levels close to the baseline control level (Figure 5c). It has been shown that the carbonyl groups may result from ROS alteration of the amino acid side chains or reactions with lipid peroxidation products that affect the protein structure [50]. This suggests that AE and CHL could reduce the carbonyl protein content both by scavenging ROS / RNS as well as by decreasing the lipid peroxidation process.…”

Fucoxanthin for Topical Administration, a Phototoxic vs. Photoprotective Potential in a Tiered Strategy Assessed by In Vitro Methods

“…After the period of incubation, we irradiated one plate with 4 J/cm 2 of UVA radiation (sun simulator), while the other one we kept in dark box. Immediately after irradiation, fluorescence intensity was measured with a microplate reader (BioTek Synergy HT, Winooski, VT, USA) at an excitation wavelength of 485 nm and emission wavelength of 528 nm [31,32]. We expressed the results as percent fluorescence intensity when compared to the untreated control irradiated, which was considered to be 100%.…”

Pharmacognostic Evaluation of Ten Species of Medicinal Importance of Cecropia: Current Knowledge and Therapeutic Perspectives

“…Collagenase levels and elastase activity were reduced by a hydroethanolic extract of C. pachystachya [66], while an ethanolic extract of the leaves of C. obtusa decreased collagenase and protein carbonyl levels [67]. Chlorogenic acid, the main component of C. obtuse, was also capable of increasing collagen and hyaluronic acid contents.…”