CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (<i>Grifola frondosa</i>) strains with different cultivation characteristics

Sato, Minoru; Miyagi, Atsuko; Yoneyama, Satoshi; Gisusi, Seiki; Tokuji, Yoshihiko; Kawai‐Yamada, Maki

doi:10.1080/09168451.2017.1387049

Cited by 18 publications

(8 citation statements)

References 31 publications

(33 reference statements)

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“…Metabolic profiling was performed on rodent muscle , heart , liver , brain , intestinal and glioma tissue, arterial cells as well as on rodent serum/plasma , urine and feces . CE‐MS was furthermore used for research on the metabolism of microorganism , plants and mushroom . Additional publications are included in Table .…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…Trehalose was reported as an essential energy source in filamentous fungi and yeast [ 66 ], and it has a key role during fermentation in Saccharomyces cerevisiae [ 67 ]. The metabolic profiling of three strains of Grifola frondosa showed that trehalose, glycerol, and glucose were the most abundant metabolites [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum

2022

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The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new Ceratorhiza hydrophila strain isolated from the submerged aquatic plant Myriophyllum spicatum . Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as Ceratorhiza hydrophila isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia , Micrococcus luteus , and Staphylococcus aureus . Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the 1 H and 1 H- 13 C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of C. hydrophila , which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using C. hydrophila as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

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“…The study revealed the role of brown mycelium in cell wall synthesis, light sensing, reduction of oxygen, and metabolic of carbohydrates [ 119 ]. Different cultivation components were compared during the cultivation of the edible mushroom Grifola frondosa (Maitake) CE–MS-based metabolomics study revealed differences in amino acid and organic acid in the strain GF433 with high amount compared to the stain Mori52, which may reveal high efficiency of the stain GF433 with high metabolite content and product efficiency [ 120 ]. Research conducted using a pea byproduct as a substrate for fungi growth with A. oryzae showed the highest ability to produce protein mass (54% of the total mass).…”

Section: Fungi As Protein and Carbohydrate Sourcementioning

confidence: 99%

Mycopharmaceuticals and Nutraceuticals: Promising Agents to Improve Human Well-Being and Life Quality

Al‐Obaidi

Jambari

Ahmad-Kamil³

2021

JoF

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Fungi, especially edible mushrooms, are considered as high-quality food with nutritive and functional values. They are of considerable interest and have been used in the synthesis of nutraceutical supplements due to their medicinal properties and economic significance. Specific fungal groups, including predominantly filamentous endophytic fungi from Ascomycete phylum and several Basidiomycetes, produce secondary metabolites (SMs) with bioactive properties that are involved in the antimicrobial and antioxidant activities. These beneficial fungi, while high in protein and important fat contents, are also a great source of several minerals and vitamins, in particular B vitamins that play important roles in carbohydrate and fat metabolism and the maintenance of the nervous system. This review article will summarize and discuss the abilities of fungi to produce antioxidant, anticancer, antiobesity, and antidiabetic molecules while also reviewing the evidence from the last decade on the importance of research in fungi related products with direct and indirect impact on human health.

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CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics

Cited by 18 publications

References 31 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum

Mycopharmaceuticals and Nutraceuticals: Promising Agents to Improve Human Well-Being and Life Quality

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