CE and CEC of nucleosides and nucleotides in food materials

Abstract

Dietary nucleosides and nucleotides play an important role in the maintenance of functions of bone marrow hematopoietic cells, intestinal mucosa, and brain. Therefore, analysis of those compounds in food is very important for improving and assuring food quality. This review summarized the application of CE and CEC in the analysis of nucleosides and nucleotides in food. The sample preparation and detection are also discussed.

A highly sensitive capillary electrophoresis method using p-nitrophenyl 5′-thymidine monophosphate as a substrate for the monitoring of nucleotide pyrophosphatase/phosphodiesterase activities

“…Due to the preconcentration effect by polycationic polymers the detection limits of analytes could be improved to the nanomolar range [1,41]. This concentration effect has been shown to work well for negatively charged analytes, including nucleotides like ATP, ADP or AMP [1,42]. pNitrophenol forms a negatively charged phenolate anion (Fig.…”

Food Analysis: Present, Future, and Foodomics

“…Other techniques as gas chromatography (GC) still have an important role in food analysis, for instance, for the analysis of volatile fractions or fatty acids in foods [20]. Electrodriven separation techniques such as capillary electrophoresis (CE) or microchip capillary electrophoresis have found important applications in food analysis as can be deduced from the many review works devoted to this topic [21,22], including the detection of genetically modified organisms [23,24], nucleosides and nucleotides in foods [25], analysis of contaminants in emerging food safety issues and food traceability [26], and food-borne pathogens [27].…”

Capillary electrophoresis coupled to mass spectrometry employing hexafluoro-2-propanol for the determination of nucleosides and nucleotide mono-, di- and tri-phosphates in baby foods

“…Regarding CE, methods have been described for the determination of nucleosides and nucleotides in food materials (Chen et al, 2010;Rodríguez-Gonzalo, Domínguez-Álvarez, Mateos-Vivas, García-Gómez, & Carabias-Martínez, 2014;Mateos-Vivas, Rodríguez-Gonzalo, Domínguez-Álvarez, et al, 2015). Soga et al (2007) have proposed a CE-MS method for the determination of three nucleotide mono-, di-and tri-phosphates (NMPs, NDPs, NTPs), among other analytes employing a common fused silica CE capillary.…”

“…The determination of these molecules is of special interest (Studzinska & Buszewski, 2013) in biochemistry (Bolin & Cardozo-Pelaez, 2007), medicine (Yeung, Ding, & Casley, 2008), food microorganisms (Zhu, Wang, Wang, Zhou, & Shi, 2016) and food analysis (Chen, Yang, Wang, & Li, 2010;Viñas et al, 2009;Wu et al, 2015;Yamaoka et al, 2010).…”