cDNA sequences of GHF9 endo-β-1,4-glucanases in terrestrial Crustacea

Gray, Michael C.; Linton, Stuart M.; Allardyce, Benjamin J.

doi:10.1016/j.gene.2017.11.030

Cited by 7 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were thus duplicated many times in a conservative manner in isopods allowing them to increase the number of expressed enzymes and thus to increase the rate of cellulose digestion. Several other crustacean species are known to express many GH9 genes, like the crayfish Cherax quadricarinatus [50] or the land crab Gecarcoidea natalis [51] which possess twenty and three forms of GH9 respectively. Furthermore, we identified β-glucosidases (EC 3.2.1.21) affiliated to GH5 family in 49 out 64 isopod transcriptomes, and lytic polysaccharide monooxygenases (AA15) in 61 transcriptomes.…”

Section: Discussionmentioning

confidence: 99%

Lignocellulose degradation in isopods: new insights into the adaptation to terrestrial life

et al. 2019

View full text Add to dashboard Cite

Background Isopods constitute a particular group of crustaceans that has successfully colonized all environments including marine, freshwater and terrestrial habitats. Their ability to use various food sources, especially plant biomass, might be one of the reasons of their successful spread. All isopods, which feed on plants and their by-products, must be capable of lignocellulose degradation. This complex composite is the main component of plants and is therefore an important nutrient source for many living organisms. Its degradation requires a large repertoire of highly specialized Carbohydrate-Active enZymes (called CAZymes) which are produced by the organism itself and in some cases, by its associated microbiota. The acquisition of highly diversified CAZymes could have helped isopods to adapt to their diet and to their environment, especially during land colonization. Results To test this hypothesis, isopod host CAZomes (i.e. the entire CAZyme repertoire) were characterized in marine, freshwater and terrestrial species through a transcriptomic approach. Many CAZymes were identified in 64 isopod transcriptomes, comprising 27 de novo datasets. Our results show that marine, freshwater and terrestrial isopods exhibit different CAZomes, illustrating different strategies for lignocellulose degradation. The analysis of variations of the size of CAZy families shows these are expanded in terrestrial isopods while they are contracted in aquatic isopods; this pattern is probably resulting from the evolution of the host CAZomes during the terrestrial adaptation of isopods. We show that CAZyme gene duplications and horizontal transfers can be involved in adaptive divergence between isopod CAZomes. Conclusions Our characterization of the CAZomes in 64 isopods species provides new insights into the evolutionary processes that enabled isopods to conquer various environments, especially terrestrial ones. Electronic supplementary material The online version of this article (10.1186/s12864-019-5825-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Lignocellulose degradation in isopods: new insights into the adaptation to terrestrial life

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For example, GH7 is exclusively found in fungi while GH9, one of the most ubiquitous and well-studied cellulase GH, can be found in organisms across the entire Tree of Life. Endogenous cellulase production in grapsoid crabs was demonstrated by transcriptomic analysis (Bui and Lee, 2015;Gan et al, 2018;Gray et al, 2018). On the other hand, there are reports of terrestrial and mangrove crustaceans producing hemicellulases such as GH5_10 endo-b-1,4-mannase and xylanase, and amylase (Pavasovic et al, 2004;An et al, 2014;Linton, 2021) that break down hemicellulose and starch, in which hemicellulose can constitute up to 30% of the dicotyledonous plant cell wall (Cosgrove, 2005;Cosgrove, 2014).…”

Section: Introductionmentioning

confidence: 99%

Widespread occurrence of endogenous cellulase production and glycosyl hydrolase in grapsoid crabs along the land-sea transition indicates high potential for mineralisation of mangrove production

Lee

Lee²

2022

Front. Mar. Sci.

View full text Add to dashboard Cite

Previous studies on mangrove carbon utilisation and mineralisation were focused on mangrove sesarmid crabs (Grapsoidea: Sesarmidae) because they are initial processors of mangrove organic carbon. Grapsoid crabs from other families and habitats were generally ignored although they also perform various levels of herbivory. It remains unclear how the herbivorous crabs utilise the production from mangrove or vascular plants because of the high C/N ratio and high structural carbon content of these materials. In this study, production of cellulase and expression of hepatopancreatic glycosyl hydrolase of grapsoid crabs were investigated in a more extensive way in terms of their taxa (15 species) and habitats (i.e., land margin, mangrove, mudflat, and subtidal area). Generally, low gastric endoglucanase (1.46-2.98 μmol min−1 ml−1) and β-glucosidase (0.06-0.61 μmol min−1 ml−1) activity was detected in 15 grapsoid crabs distributed along the land-sea transition. The activity of both enzymes was significantly affected by the taxonomic affiliation and habitat of the crabs: endoglucanase in sesarmids > varunids > grapsids; and land margin, mangrove and limnic habitats > mudflat and subtidal area. For β-glucosidase: sesarmids > varunids and grapsids; and land and limnic > mangrove and subtidal > mudflat. Our detection of gastric cellulases in mudflat and subtidal grapsoid crabs has rarely been reported. These results show that there is a need to re-evaluate the role of crabs from these habitats in mangrove/vascular plant carbon mineralisation. Further, low cellulase activity could be a common trait among crabs along the land-sea transition and the importance of cellulase in vascular plant carbon utilisation could have been previously over-rated. Glycosyl hydrolase family 9 (GH9) genes were identified in the hepatopancreatic transcriptome of all 15 species, indicating endogenous production of cellulase. In addition, the presence of other digestive carbohydrate-active enzymes (CAZymes), including GH5_10, GH13, GH13_24, and GH16, suggests that a capacity for the utilisation of carbon sources other than cellulose by the grapsoid crabs, which may enable sufficient assimilation of vascular plant carbon despite the low cellulase activity.

show abstract

“…Glycoside hydrolase family 9 (GH9) genes (http://www.cazy.org/), which primarily code for endo-β-1,4-glucanases, have been identi ed in various plant species, including, but not limited to, Arabidopsis thaliana, Oryza sativa, Populus L., and these genes were shown to be associated with diverse aspects of plant growth and development (Cantarel et al 2009;Dheilly et al 2016;Xie et al 2013;Yu et al 2014). The crystal structure showed a high degree of conservation in both secondary and tertiary structures, as well as catalytic and carbohydrate binding amino acid residues in certain species (Gray et al 2018). Plant-derived GH9 enzymes typically consist of 441 to 694 amino acid residues, with a calculated and observed molecular mass ranging from 42 to 70 kDa.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of GH9 gene family and the assessment of its role during fruit abscission zone formation in Vaccinium ashei

Wang

Liao

et al. 2023

Plant Cell Rep

View full text Add to dashboard Cite

Glycosyl hydrolase family 9 (GH9) cellulases play a crucial role in both cellulose synthesis and hydrolysis during plant growth and development. Despite this importance, there is currently no study on the involvement of GH9-encoding genes, speci cally VaGH9s, in abscission zone formation of rabbiteye blueberries (Vaccinium ashei). In this study, we identi ed a total of 61 VaGH9s in the genome, which can be classi ed into three subclasses based on conserved motifs and domains, gene structures, and phylogenetic analyses. Our synteny analysis revealed that VaGH9s are more closely related to the GH9s of Populus L. than to those of Arabidopsis, Vitis vinifera, and Citrus sinensis. In-silico structural analysis predicted that most of VaGH9s are hydrophilic, and localized in cell membrane and/or cell wall, and the variable sets of cis-acting regulatory elements and functional diversity with four categories of stress response, hormone regulation, growth and development, and transcription factor-related elements are present in the promoter sequence of VaGH9s genes. Transcriptomic analysis showed that there were 22 differentially expressed VaGH9s in fruit abscission zone tissue at the veraison stage, and the expression of VaGH9B2 and VaGH9C10 was continuously increased during fruit maturation, which were in parallel with the increasing levels of cellulase activity and oxidative stress indicators, suggesting that they are involved in the separation stage of fruit abscission in Vaccinium ashei. Our work identi ed 22 VaGH9s potentially involved in different stages of fruit abscission and would aid further investigation into the molecular regulation of abscission in rabbiteye blueberries fruit. Key messageThe genomic location and stage-speci c expression pattern of GH9 genes reveal their critical roles during fruit abscission zone formation in Vaccinium ashei.

show abstract

cDNA sequences of GHF9 endo-β-1,4-glucanases in terrestrial Crustacea

Cited by 7 publications

References 37 publications

Lignocellulose degradation in isopods: new insights into the adaptation to terrestrial life

Lignocellulose degradation in isopods: new insights into the adaptation to terrestrial life

Widespread occurrence of endogenous cellulase production and glycosyl hydrolase in grapsoid crabs along the land-sea transition indicates high potential for mineralisation of mangrove production

Genome-wide identification of GH9 gene family and the assessment of its role during fruit abscission zone formation in Vaccinium ashei

Contact Info

Product

Resources

About