cDNA cloning of an alginate lyase from abalone, Haliotis discus hannai

An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai

“…Biochemical properties of sea hare mannosidase will be published elsewhere. On the other hand, we have reported various kinds of molluscan polysaccharidedegrading enzymes, e.g., alginate lyase, cellulase, and -1,3-glucanase (Shimizu et al, 2003;Suzuki et al, 2003;Suzuki et al, 2006;Hata et al, 2009;Kumagai and Ojima, 2010). Studies on these enzymes are expected to provide us the useful information about the degradation process of seaweeds' polysaccharides, which are expected as functional polysaccharides for human and a potential biomass in future.…”

Enzymatic properties and the primary structure of a β-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai

“…The composition of enzymes in the digestive fluid is closely related to the feeding habits of the animals. For example, digestive fluid of marine gastropods such as abalone and sea hare contains alginate lyase, amylase, cellulase, and mannanase, which can depolymerize seaweeds' alginic acid, dextran, cellulose, and -mannan, respectively (Shimizu et al, 2003;Suzuki et al, 2003;Andreotti et al, 2005 andSuzuki et al, 2006;Ootsuka et al, 2006;Nikapitiya et al, 2009). The oligosaccharides and monosaccharides produced by these enzymes are considered to be directly assimilated by the animals themselves or indirectly through fermentation by intestinal bacteria (Erasmus et al, 1997;Sawabe et al, 2003).…”

Isolation and characterization of two types of β-1,3-glucanases from the common sea hare Aplysia kurodai

“…To date, β-1,3-glucanases have been found in bacteria, fungi, plants, insects, and invertebrate animals (Privalova et al, 1978;Takami et al, 1998;Shimizu et al, 2003;Suzuki et al, 2003;Kovalchuk et al, 2006;Suzuki et al, 2006). Recently, we isolated endolytic β-1,3-glucanases (EC 3.2.1.6) from the ezo-giant scallop…”