An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai

Characterization of a β-D-mannosidase from a marine gastropod, Aplysia kurodai

“…During the purification of β-1,4-mannanase from A. kurodai (Zahura et al, 2010), we noticed the occurrence of β-mannosidase(s) in the crude enzyme preparation. In the present study, we successfully purified the β-mannosidase, AkMnsd, from the crude enzyme.…”

“…As for the mannan degradation, AkMan splits the internal β-1,4-mannosyl linkages of the seaweeds' β-mannan producing tri-and disaccharides. But this enzyme could not produce mannose (Zahura et al, 2010).…”

“…In aquatic environment, mannans in algal fronds and their detritus are consumed not only by microorganisms but also by benthic animals such as annelids, arthropods and mollusks. These invertebrates also possess mannandegrading enzymes and assimilate the mannans as a carbon and energy sources (Chuang and Yang, 1991;Xu et al, 2002a;Song et al, 2008;Ootsuka et al, 2006;Zahura et al, 2010). For complete depolymerization of mannan, at least two types of mannan-degrading enzymes are necessary.…”

“…Recently, we isolated and characterized AkMan, a -mannanase from a common sea hare Aplysia kurodai, and determined its primary structure by cDNA cloning (Zahura et al, 2010 and. A. kurodai is known as a typical herbivorous marine mollusk which possesses various kinds 5 of polysaccharide-degrading enzymes, e.g., alginate lyase laminarinase (Kumagai and Ojima, 2010) in addition to -mannanase (Zahura et al, 2010 and.…”

“…A. kurodai is known as a typical herbivorous marine mollusk which possesses various kinds 5 of polysaccharide-degrading enzymes, e.g., alginate lyase laminarinase (Kumagai and Ojima, 2010) in addition to -mannanase (Zahura et al, 2010 and.…”