cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells

Yamakawa, Toru; Miyata, Satoshi; Ogawa, Naoki; Koshikawa, Naohiko; Yasumitsu, Hidetaro; Kanamori, Tatsuyuki; Miyazaki, Kaoru

doi:10.1016/s0167-4781(97)00149-8

Cited by 36 publications

(36 citation statements)

References 20 publications

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“…In addition, cystatin C as well as S100P have recently been identified as markers of PDAC in a global proteome analysis of pancreatic juice samples (Gronborg et al, 2004). Other candidates identified in our analysis included the extracellular protease inhibitor 15 (PI15), which has been proposed as a marker for neuroblastoma and glioblastoma (Yamakawa et al, 1998), galectin 4 (LGALS4), which is secreted in a soluble form by many epithelial cancer cells and has been proposed as a marker for breast and liver tumours (Huflejt and Leffler, 2004), and the procollagen (type III) N-endopeptidase (PCOLN3), a matrix metalloproteinase not previously implicated in carcinogenesis. In summary, the combined use of tissue microdissection, RNA amplification and global microarray analysis has proven to be a powerful tool for profiling the molecular changes associated with the progression of normal pancreatic duct cells to invasive ductal adenocarcinoma.…”

Section: Discussionmentioning

confidence: 92%

Transcriptome analysis of microdissected pancreatic intraepithelial neoplastic lesions

Buchholz

Braun

Heidenblut³

et al. 2005

Oncogene

169

121

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Pancreatic ductal adenocarcinoma (PDAC) carries the most dismal prognosis of all solid tumours. Both the late clinical presentation of patients, due to lack of early symptoms, as well as the rapid and aggressive course of the disease contribute to the extremely high mortality of this malignancy. Recently, a multistep progression model for PDAC integrating morphological, clinical and molecular evidence has been proposed. Putative precursor lesions, termed pancreatic intraepithelial neoplasia (PanIN), are classified into three different grades (PanIN-1 through -3) based on the degree of cellular atypia they display. We have conducted large-scale expression profiling analyses of microdissected cells from normal pancreatic ducts, PanINs of different grades and PDACs using whole-genome oligonucleotide microarrays. Verification of hybridisation results for selected genes was performed using quantitative real-time PCR and immunohistochemical analyses on PanIN tissue microarrays.Comparison of the expression profiles demonstrated that the greatest changes in gene expression occur between PanIN stages 1B and 2, suggesting that PanIN-2 may represent the first truly preneoplastic stage in PDAC progression. Our results identify a large number of potential target genes for the development of novel molecular diagnostic and therapeutic tools for the prevention and early diagnosis of PDAC and provide novel insights into the pathophysiological mechanisms involved in tumour progression in the pancreas. Oncogene (2005) 24, 6626-6636.

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Section: Discussionmentioning

confidence: 92%

Transcriptome analysis of microdissected pancreatic intraepithelial neoplastic lesions

Buchholz

Braun

Heidenblut³

et al. 2005

Oncogene

169

121

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“…Based on its intracellular location within the specific granules, SGP28 has been hypothesized to function either as an antimicrobial protein or as a type of matrix protease. Another related 25 kDa protein (P25TI), frequently expressed on human neuroblastoma and glioblastoma cell lines and also present at low levels in the brain, placenta and lymphocytes, was characterized as exhibiting weak trypsin-inhibiting activity, but its sequence has no homology to other proteinase inhibitors [88]. PR-1 proteins from plants have been tested for proteinaseinhibitory activity, but none has been reported.…”

Section: T  3 Homology Of Pr-1 Type Proteins From Plants and Othmentioning

confidence: 99%

The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins

Loon

Strien

1999

Physiological and Molecular Plant Pathology

1,652

929

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“…The presence of such an evolutionarily diverse, yet conserved, structure is suggestive of a common function, although the identification of this function remains a challenge. Data from a range of species, however, imply roles for CAPs as antifungal agents in plants (Niderman et al, 1995); venoms of wasps and as a significant allergen in humans (King et al, 1978;Lu et al, 1993); as a pro-tease in cone snails (Milne et al, 2003); as a protease inhibitor in human glioblastoma cells (Yamakawa et al, 1998); as an anti-coagulant protein in lampreys (Ito et al, 2007); and, as outlined in more detail below, in mediating cell-cell binding in mammals.…”

Section: Introductionmentioning

confidence: 99%

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Reddy¹,

Gibbs²,

Merriner³

et al. 2008

Developmental Dynamics

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The Cysteine-RIch Secretory Proteins (CRISPs) are abundantly produced in the male reproductive tract of mammals and within the venom of reptiles and have been shown to regulate ion channel activity. CRISPs, along with the Antigen-5 proteins and the Pathogenesis related-1 (Pr-1) proteins, form the CAP superfamily of proteins. Analyses of EST expression databases are increasingly suggesting that mammalian CRISPs are expressed more widely than in the reproductive tract. We, therefore, conducted a reverse transcription PCR expression profile and immunohistochemical analyses of 16 mouse tissues to define the sites of production of each of the four murine CRISPs. These data showed that each of the CRISPs have distinct and sometimes overlapping expression profiles, typically associated with the male and female reproductive tract, the secretory epithelia of exocrine glands, and immune tissues including the spleen and thymus. These investigations raise the potential for a role for CRISPs in general mammalian physiology. Developmental Dynamics 237:3313-3323, 2008.

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cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells

Cited by 36 publications

References 20 publications

Transcriptome analysis of microdissected pancreatic intraepithelial neoplastic lesions

Transcriptome analysis of microdissected pancreatic intraepithelial neoplastic lesions

The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

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