cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis

Kuro‐o, Makoto; Nagai, Ryozo; Nakahara, Ken; Katoh, Hirotaka; Tsai, Rong Chi; Tsuchimochi, Hidetsugu; Yazaki, Yoshio; Ohkubo, Akihiro; Takaku, Fumimaro

doi:10.1016/s0021-9258(19)67861-0

Cited by 270 publications

(31 citation statements)

References 30 publications

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“…33,34 The monoclonal antibodies against MHC isoforms were generated and kindly provided by Prof Ryozo Nagai (Gunma University, Japan). [29][30][31][32][33] The specificities of the primary antibodies we used in the present study were previously confirmed by means of Western analysis and immunohistochemistry. [23][24][25][26][27][28][29][30][31][32]…”

Section: Antibodies Used For Immunohistochemistrymentioning

confidence: 56%

“…SMemb is a nonmuscle MHC isoform and is expressed in SMCs at embryonic and neonatal stages and when they are phenotypically modulated toward the dedifferentiated-type but down regulated in the adults. [29][30][31][32] SM2 is shown to be expressed in a reversed manner. Therefore, SMemb is a useful marker for the dedifferentiated-type SMCs, and SM2 is a marker for the differentiated-type.…”

Section: Antibodies Used For Immunohistochemistrymentioning

confidence: 96%

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Association of smooth muscle cell phenotypic modulation with extracellular matrix alterations during neointima formation in rabbit vein grafts

Zhang

Bai

Sawa

et al. 1999

Journal of Vascular Surgery

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The neointima in the vein graft was formed initially by means of migration and proliferation of the phenotypically modulated, dedifferentiated-type SMCs and continued to thicken by means of sustained ECM accumulation, including type I collagen and decorin, in association with the prolonged presence of the dedifferentiated-type SMCs. These chronologic features in cell kinetics and ECM accumulation may contribute to the frequent occurrence of graft wall thickening that occurs in the vein grafts.

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Section: Antibodies Used For Immunohistochemistrymentioning

confidence: 56%

Section: Antibodies Used For Immunohistochemistrymentioning

confidence: 96%

Association of smooth muscle cell phenotypic modulation with extracellular matrix alterations during neointima formation in rabbit vein grafts

Zhang

Bai

Sawa

et al. 1999

Journal of Vascular Surgery

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“…Immunoblotting. The differentiation state of smooth muscle cells was determined by assessing expression of smooth muscle-specific contractile proteins smooth muscle myosin heavy chain isoforms 1 (SM1) and 2 (SM2) in coronary arterioles (20,22). The levels of downstream effectors of adiponectin, AMP-activated kinase (2,6,8,14,29,32,39) and ribosomal protein S6 (12,33), were assessed.…”

Section: Animalsmentioning

confidence: 99%

Effects of age and exercise training on coronary microvascular smooth muscle phenotype and function

Muller‐Delp

Hotta

Chen

et al. 2018

Journal of Applied Physiology

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Coronary microvascular function and blood flow responses during acute exercise are impaired in the aged heart but can be restored by exercise training. Coronary microvascular resistance is directly dependent on vascular smooth muscle function in coronary resistance arterioles; therefore, we hypothesized that age impairs contractile function and alters the phenotype of vascular smooth muscle in coronary arterioles. We further hypothesized that exercise training restores contractile function and reverses age-induced phenotypic alterations of arteriolar smooth muscle. Young and old Fischer 344 rats underwent 10 wk of treadmill exercise training or remained sedentary. At the end of training or cage confinement, contractile responses, vascular smooth muscle proliferation, and expression of contractile proteins were assessed in isolated coronary arterioles. Both receptor- and non-receptor-mediated contractile function were impaired in coronary arterioles from aged rats. Vascular smooth muscle shifted from a differentiated, contractile phenotype to a secretory phenotype with associated proliferation of smooth muscle in the arteriolar wall. Expression of smooth muscle myosin heavy chain 1 (SM1) was decreased in arterioles from aged rats, whereas expression of phospho-histone H3 and of the synthetic protein ribosomal protein S6 (rpS6) were increased. Exercise training improved contractile responses, reduced smooth muscle proliferation and expression of rpS6, and increased expression of SM1 in arterioles from old rats. Thus age-induced contractile dysfunction of coronary arterioles and emergence of a secretory smooth muscle phenotype may contribute to impaired coronary blood flow responses, but arteriolar contractile responsiveness and a younger smooth muscle phenotype can be restored with late-life exercise training. NEW & NOTEWORTHY Aging impairs contractile function of coronary arterioles and induces a shift of the vascular smooth muscle toward a proliferative, noncontractile phenotype. Late-life exercise training reverses contractile dysfunction of coronary arterioles and restores a young phenotype to the vascular smooth muscle.

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“…SMemb is predominantly expressed in undifferentiated vascular SMCs and is reduced during vascular development. 4,11 SMemb is especially important for the investigation of the synthetic-state SMCs. Therefore, we measured the number of anti-SMemb antibody positive cells in the intima on POD 28.…”

Section: B Amentioning

confidence: 99%

“…in their synthetic state produce collagen, which leads to intimal hyperplasia. [3][4][5] Thus, to understand the process of phonetically modulated SMCs in the development of intimal hyperplasia, the investigation of smooth muscle (SM) phenotypes in animal models after grafting is important.…”

mentioning

confidence: 99%

Hepatocyte growth factor prevents intimal hyperplasia in rabbit carotid expanded polytetrafluoroethylene grafting

Harada

Takenaka

Ikenaga

et al. 2002

Journal of Vascular Surgery

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cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis

Cited by 270 publications

References 30 publications

Association of smooth muscle cell phenotypic modulation with extracellular matrix alterations during neointima formation in rabbit vein grafts

Association of smooth muscle cell phenotypic modulation with extracellular matrix alterations during neointima formation in rabbit vein grafts

Effects of age and exercise training on coronary microvascular smooth muscle phenotype and function

Hepatocyte growth factor prevents intimal hyperplasia in rabbit carotid expanded polytetrafluoroethylene grafting

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