CDCA5 overexpression is an Indicator of poor prognosis in patients with hepatocellular carcinoma (HCC)

Abstract

BackgroundAccurate and early prognosis of disease is essential to clinical decision making, particularly in diseases, such as HCC, that are typically diagnosed at a late stage in the course of disease and therefore carry a poor prognosis. CDCA5 is a cell cycle regulatory protein that has shown prognostic value in several cancers.MethodsWe retrospectively evaluated 178 patients with HCC treated with curative liver resection between September 2009 and September 2012 at Nanchong Central Hospital in Nanchong, Sichuan Province, China. Patients were screened for their CDCA5 expression levels and assigned to either the high or low expression group. Patient demographics, comorbidities, clinicopathologic data, such as tumor microvascular invasion status and size, and long-term outcomes were compared between the two groups. The effect of CDCA5 on the proliferation of liver cancer cells was analyzed using in vitro and in vivo assays.ResultsThe present study found that increased CDCA5 expression was associated with increased tumor diameter and microvascular invasion in HCC. It was also found that CDCA5 overexpression may be associated with liver cancer cells. Additionally, this study confirmed that CDCA5 expression was increased in HCC tissue versus normal liver tissue, that CDCA5 expression was associated with decreased survival and that CDCA5 knockdown using shRNA led to cell cycle arrest in the G2/M phase.ConclusionsThese findings suggest that CDCA5 expression is associated with poor prognosis in patients with hepatocellular carcinoma.

Identification of Hepatocellular Carcinoma-Related Potential Genes and Pathways Through Bioinformatic-Based Analyses

“…Cell division cycle-associated 5 (CDCA5) serves an important role in promoting colorectal cancer progression by activating the ERK signaling pathway (Shen et al, 2019). Additionally, CDCA5 could be associated with HCC (Tian et al, 2018), breast cancer (Phan et al, 2018), and lung carcinogenesis (Nguyen et al, 2010). Centromere protein U (CENPU) promotes nonsmall cell lung cancer proliferation involving the Wnt/b-catenin signaling pathway and predicts poor survival through the expression of FOXM1 (Wang et al, 2018;Zhang et al, 2018).…”

Identification and Analysis of Genes Associated with Epithelial Ovarian Cancer by Integrated Bioinformatics Methods

“…Besides, CDCA5 was found to be transcribed by E2F1, and could promote oncogenesis by enhancing cell proliferation and inhibiting apoptosis via the AKT pathway in HCC [18]. Another research found that increased CDCA5 expression was associated with increased tumor diameter and microvascular invasion in HCC [19]. Furthermore, silencing of CDCA5 inhibited cell proliferation and induced G2/M cycle arrest in vitro, and CDCA5 down-regulation in xenograft model impeded HCC growth in vivo.…”

Construct ion of co - expression modules related to survival by WGCNA and identif ication of potential prognostic biomarkers in glioblastoma

“…In order to explore the specific mechanism of the effect of these genes on survival, we screened the top 10 genes (CDC20, NCAPH, CDCA5, BUB1, CDCA8, PBK, KIF2C, TPX2, TTK and TOP2A). By retrieving related literature, we found that CDCA5 and CDCA8, as important regulatory proteins in the cell cycle in cancer, were recognized as oncogenes [26][27][28][29]. However, compared with other genes, there scarcely no reports about the mechanism of CDCA5 and CDCA8 with GBM.…”