2004
DOI: 10.1182/blood-2004-01-0104
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CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells

Abstract: We analyzed herein whether members of the tetraspanin superfamily are involved in human immature dendritic cell (DC) functions such as foreign antigen internalization, phagocytosis, and cell migration. We show that CD63, CD9, CD81, CD82, and CD151 are present in immature DCs. Whereas CD9 and CD81 are mostly expressed at the cell surface, CD63 and CD82 are also located in intracellular organelles. Complexes of monoclonal antibody (Mab) FC-5.01-CD63 or Fab-5.01-CD63 were rapidly translocated "outside-in" and fol… Show more

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Cited by 113 publications
(110 citation statements)
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References 48 publications
(67 reference statements)
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“…21 Tetraspanin complexes act as molecular facilitators in many cellular processes, 35,36 and different partner proteins can become clustered together with CD63. 37 It has been proposed that membrane proteins such as the H,K-ATPase 25 and membrane type 1-matrix metalloproteinase 23,38 are transported by CD63.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…21 Tetraspanin complexes act as molecular facilitators in many cellular processes, 35,36 and different partner proteins can become clustered together with CD63. 37 It has been proposed that membrane proteins such as the H,K-ATPase 25 and membrane type 1-matrix metalloproteinase 23,38 are transported by CD63.…”
Section: Discussionmentioning
confidence: 99%
“…31,32 Therefore, the possible presence of proteoglycans was examined, as previously described by Lemansky et al 32 COS cells coexpressing NE and GFP-CD63 were incubated with radioactive sulfate, immunoprecipitated with anti-cproNE, and subjected to SDS-PAGE followed by fluorography. ProNE did not pull down any 35 S-labeled macromolecules (data not shown), indicating that proteoglycan was not present.…”
Section: Prone and Cd63 Colocalize In Cos Cellsmentioning
confidence: 92%
“…Here we have addressed the behavior of CD63 in the maturation of phagosomes using live cell imaging. To date, characterization of CD63 has been accomplished in human cell lines, largely through biochemical analysis (12,16,27). The data presented here focus on the behavior of CD63 and class II MHC in live primary cultures of mouse APCs in the context of antigen capture by phagocytosis.…”
Section: Discussionmentioning
confidence: 99%
“…More recently, the focus has shifted to its interaction with class II MHC and its role in antigen presentation. In professional APCs such as dendritic cells (DCs), subcellular fractionation and biochemical analysis show that human CD63 segregates into class II MHC-rich domains within the endocytic pathway and cell surface (10)(11)(12). Furthermore, this tetraspanin protein preferentially resides in membrane patches enriched for particular class II MHC-peptide complexes (13).…”
mentioning
confidence: 99%
“…Moreover, growing evidence indicates tetraspanins to be critically involved in the regulation of cell motility [18][19][20]. In fact, Mantegazza and colleagues recently demonstrated the tetraspanin CD63 to slow down migration of DC possibly via interfering with surface expression of integrins.…”
Section: Supporting Information Available Onlinementioning
confidence: 99%