2006
DOI: 10.1073/pnas.0607528103
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Recruitment of CD63 toCryptococcus neoformansphagosomes requires acidification

Abstract: The subcellular localization of the cluster of differentiation 63 (CD63) tetraspanin and its interaction with the class II MHC antigen presentation pathway were examined in the context of phagocytosis by live cell imaging, by using monomeric red fluorescent protein-tagged mouse CD63 expressed in primary bone marrow-derived cell cultures. Upon phagocytosis of Cryptococcus neoformans and polystyrene beads, CD63 was recruited selectively to C. neoformans-containing phagosomes in a MyD88-independent acidification-… Show more

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Cited by 61 publications
(66 citation statements)
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References 47 publications
(40 reference statements)
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“…A lentivirus expressing CD63 fused to a cherryfluorescent protein tag was prepared as described in ref. 32.…”
Section: Methodsmentioning
confidence: 99%
“…A lentivirus expressing CD63 fused to a cherryfluorescent protein tag was prepared as described in ref. 32.…”
Section: Methodsmentioning
confidence: 99%
“…Lentiviral Transduction-HEK293T cells were used to generate lentivirus as described (28). GFP-Dectin-1 or GFP-Dectin-1⌬Y15 was subcloned into pHAGE II, a fourth-generation lentiviral self-inactivating nonreplicative vector (28) (29).…”
Section: Methodsmentioning
confidence: 99%
“…Phagosomal maturation is not a default pathway, but rather the nature of this membrane compartment is determined by its content (6). We have shown selective recruitment of class II MHC molecules and CD63, a member of the tetraspanin superfamily, to phagosomes containing the pathogenic yeast Cryptococcus neoformans (CN) (7). Peptide-loaded class II MHC molecules translocate from endosomal compartments to the cell surface by long endolysosomal tubules to engage Ag-specific T cells (8,9).…”
Section: Endritic Cells (Dcs)mentioning
confidence: 99%
“…An Ab against CD63 inhibited IgE-mediated histamine release, suggesting a role for CD63 in mast cell biology (21). In APCs, CD63 and another lysosomal membrane protein, LAMP1 (lysosomal-associated membrane protein), are both recruited to yeast-containing phagosomes (7). CD63 requires acidification of the phagosome for recruitment, while LAMP1 does not (7).…”
Section: Endritic Cells (Dcs)mentioning
confidence: 99%