CD4 T-cell transcriptome expression reversal of the lncRNA-mRNA co-expression network in elite controllervs.normal-process HIV patients

Abstract

21 22 42 will help researchers and clinicians to understand the interrelationship between HIV 43 and the host. In the present study, we focused on the interactions and functional 44 relationships between significantly differentially expressed long non-coding RNAs 3 45 (lncRNAs) and mRNAs in ECs vs. normal-process patients (NPs). RNA-sequencing 46 was performed for six representative samples of CD4 T-cells. Using the Pearson 47 correlation test, an lncRNA-mRNA co-expression network was constructed. Several 48 new regulatory relationships between transcripts were revealed that might be closely 49 related to the ability of ECs to maintain a low viral load for long periods without 50 anti-viral treatment. For example, lncRNA C3orf35 was upregulated in ECs vs. NPs 51 and was positively related to downregulation of GNG2 mRNA (encoding G protein 52 subunit gamma 2), which functions in chemokine signaling pathways and HIV-1 53infection. Overall, we identified certain interesting genetic interactions that will 54 provide information about the mechanism of host suppression of viral replication. 55 56

Long Non-coding RNAs Mechanisms of Action in HIV-1 Modulation and the Identification of Novel Therapeutic Targets

“…Additionally, one could perform RNA-seq analyses at the different phases of HIV-1 infection in target human T cells that would aid in identifying unique signatures. For example, Chen et al (2019) determined the lncRNA-mRNA co-expression profiles in elite controllers versus normal-process HIV-1 patients [22], and found that these profiles were differentially enriched in immune response-regulating signaling and apoptosis pathways [22]. Alternatively, one could screen based on lncRNA mechanism.…”

“…This subpopulation of CD4+ T cells remain a challenge to current treatment strategies that are only able to effectively target actively replicating cells [20]. Over the past decade, the role of lncRNAs in modulating HIV-1 has broadened our understanding of how host factors can maintain viral latency and facilitate HIV-1 replication in target host cells [21][22][23][24][25].…”