CD34 human hematopoietic progenitor cell line, MUTZ-3, differentiates into functional osteoclasts

Ciraci, Elisa; Barisani, Donatella; Parafioriti, Antonina; Formisano, Giuseppe; Arancia, Giuseppe; Bottazzo, G. F.; Berardi, Anna C.

doi:10.1016/j.exphem.2007.03.003

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…pre-odontoblasts and pre-osteoblasts for healing. That AMBN gene expression is also present in CD34 + cells do not come as a surprise since CD34 + cells have been shown to have the potential to differentiate into both osteoblasts [45–48] as well as monocytes and osteoclast precursors [49,50]. …”

Section: Discussionmentioning

confidence: 99%

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair

Tamburstuen

Reseland

Spahr

et al. 2011

Bone

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Ameloblastin is mainly known as a dental enamel protein, synthesized and secreted into developing enamel matrix by the enamel-forming ameloblasts. The function of ameloblastin in tooth development remains unclear, but it has been suggested to be involved in processes varying from regulating crystal growth to activity as a growth factor or partaking in cell signaling. Recent studies suggest that some enamel matrix proteins also might have important functions outside enamel formation. In this context ameloblastin has recently been reported to induce dentin and bone repair, as well as being present in the early bone and cartilage extracellular matrices during embryogenesis. However, what cells express ameloblastin in these tissues still remain unclear. Thus, the expression of ameloblastin was examined in cultured primary mesenchymal cells and in vivo during healing of bone defects in a “proof of concept” animal study. The real time RT-PCR analysis revealed human ameloblastin (AMBN) mRNA expression in human mesenchymal stem cells and primary osteoblasts and chondrocytes. Expression of AMBN mRNA was also confirmed in human CD34 positive cells and osteoclasts. Western and dot blot analysis of cell lysates and medium confirmed the expression and secretion of ameloblastin from mesenchymal stem cells, primary human osteoblasts and chondrocytes. Expression of ameloblastin was also detected in newly formed bone in experimental bone defects in adult rats. Together these findings suggest a role of this protein in early bone formation and repair.

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Section: Discussionmentioning

confidence: 99%

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair

Tamburstuen

Reseland

Spahr

et al. 2011

Bone

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“…Numerous studies have reported that osteoclasts (OCs) can be generated from colony forming unit-granulocyte-macrophage progenitors as well as from the PBMC population [68-77]. Costa-Rodrigues and colleagues [78] reported that the whole PBMC fraction and CD14+ PBMCs, but not CD14- PBMCs, survived in the absence of osteoclastogenic factors and exhibited spontaneous osteoclastogenesis as detected by cell type-specific protein and gene expression, TRAP activity, calcium phosphate resorption, and production of F-actin rings.…”

Section: Evidence For the Multi-differentiation Potential Of Pbmcsmentioning

confidence: 99%

The multi-differentiation potential of peripheral blood mononuclear cells

Zhang

Huang

2012

Stem Cell Res Ther

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Peripheral blood is a large accessible source of adult stem cells for both basic research and clinical applications. Peripheral blood mononuclear cells (PBMCs) have been reported to contain a multitude of distinct multipotent progenitor cell populations and possess the potential to differentiate into blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, osteoblasts, osteoclasts, epithelial cells, neural cells, or myofibroblasts under appropriate conditions. Furthermore, transplantation of these PBMC-derived cells can regenerate tissues and restore function after injury. This mini-review summarizes the multi-differentiation potential of PBMCs reported in the past years, discusses the possible mechanisms for this multi-differentiation potential, and describes recent techniques for efficient PBMC isolation and purification.

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“…Osteoclasts are formed through multiple fusions of their mononuclear precursors that have haematopoietic origin and are derived from the monocyte/macrophage lineage (6,7). It has been observed that osteoclasts can be generated from colony‐forming unit granulocyte macrophage progenitors (8,9) and osteoclastic cells can also be obtained from monocytes present in peripheral blood (10). Monocytes are a heterogenous population (11) that comprises of, in addition to osteoclastic progenitors, precursors of several other cell types, such as macrophages (12), endothelial cells (13) and dendritic cells (14).…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14+ and CD14− cell fractions

Costa‐Rodrigues¹,

Fernandes²,

Fernandes³

2011

Cell Proliferation

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CD34 human hematopoietic progenitor cell line, MUTZ-3, differentiates into functional osteoclasts

Cited by 8 publications

References 45 publications

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair

The multi-differentiation potential of peripheral blood mononuclear cells

Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14+ and CD14− cell fractions

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