Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14+ and CD14− cell fractions

Costa‐Rodrigues, João; Fernandes, Anabela C.; Fernandes, Maria Helena

doi:10.1111/j.1365-2184.2011.00768.x

Cited by 31 publications

(26 citation statements)

References 50 publications

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“…Peripheral blood mononuclear cells (PBMC) were isolated as described previously [Costa-Rodrigues et al, 2011b]. Shortly, blood was diluted (1:1) with PBS and applied …”

Section: Isolation and Culture Of Human Peripheral Blood Mononuclear mentioning

confidence: 99%

Variability of the paracrine‐induced osteoclastogenesis by human breast cancer cell lines

Costa‐Rodrigues

Moniz

Teixeira

et al. 2012

J of Cellular Biochemistry

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Breast cancer frequently metastasizes to the bone, often leading to the formation of osteolytic lesions. This work compares the paracrine-induced osteoclastogenesis mediated by four human breast cancer cell lines, the estrogen-receptor positive T47D and MCF-7 and the estrogen-negative SK-BR-3 and Hs-578T cell lines. Human osteoclast precursor cells were cultured in the presence of conditioned media from the breast cancer cell lines (10% and 20%), collected at different culture periods (48 h, 7 days, and 14 days). Cultures performed in the absence or the presence of M-CSF and RANKL served as negative and positive control, respectively. Results showed that the cell lines differentially expressed several osteoclastogenic genes. All cell lines exhibited a significant osteoclastogenic potential, evidenced by a high TRAP activity and number of osteoclastic cells, expression of several osteoclast-related genes, and, particularly, a high calcium phosphate resorption activity. Differences among the osteoclastogenic potential of the cell lines were noted. T47D and MCF-7 cell lines displayed the highest and the lowest osteoclastogenic response, respectively. Despite the variability observed, MEK and NF-κB signaling pathways, and, at a lesser extent, PGE2 production, seemed to have a central role on the observed osteoclastogenic response. In conclusion, the tested breast cancer cell lines exhibited a high osteoclastogenic potential, although with some variability on the cell response profile, a factor to be considered in the development of new therapeutic approaches for breast cancer-induced bone metastasis.

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“…Peripheral blood mononuclear cells (PBMC) were isolated as described previously [Costa-Rodrigues et al, 2011b]. Shortly, blood was diluted (1:1) with PBS and applied …”

Section: Isolation and Culture Of Human Peripheral Blood Mononuclear mentioning

confidence: 99%

Variability of the paracrine‐induced osteoclastogenesis by human breast cancer cell lines

Costa‐Rodrigues

Moniz

Teixeira

et al. 2012

J of Cellular Biochemistry

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“…Costa-Rodrigues and colleagues [78] reported that the whole PBMC fraction and CD14+ PBMCs, but not CD14- PBMCs, survived in the absence of osteoclastogenic factors and exhibited spontaneous osteoclastogenesis as detected by cell type-specific protein and gene expression, TRAP activity, calcium phosphate resorption, and production of F-actin rings. Unlike CD14+ PBMCs, the total PBMC population was able to express macrophage colony-stimulating factor and RANKL (receptor activator of nuclear factor kappa-B ligand), as well as tumor necrosis factor-a, granulocyte-macrophage colony-stimulating factor, IL-1, IL-6, and IL-17, which are necessary for osteoclastogenesis [8,69].…”

Section: Evidence For the Multi-differentiation Potential Of Pbmcsmentioning

confidence: 99%

The multi-differentiation potential of peripheral blood mononuclear cells

Zhang

Huang

2012

Stem Cell Res Ther

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Peripheral blood is a large accessible source of adult stem cells for both basic research and clinical applications. Peripheral blood mononuclear cells (PBMCs) have been reported to contain a multitude of distinct multipotent progenitor cell populations and possess the potential to differentiate into blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, osteoblasts, osteoclasts, epithelial cells, neural cells, or myofibroblasts under appropriate conditions. Furthermore, transplantation of these PBMC-derived cells can regenerate tissues and restore function after injury. This mini-review summarizes the multi-differentiation potential of PBMCs reported in the past years, discusses the possible mechanisms for this multi-differentiation potential, and describes recent techniques for efficient PBMC isolation and purification.

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“…48 In a previous work, the comparison of PBMC with their CD14+ and CD14− subpopulations showed that PBMC outputted a higher degree of osteoclastogenesis, either as absolute values or after normalization by the protein content. 21 This study suggested that although osteoclast precursors reside in the CD14+ population, other populations present in PBMC have the ability to modulate osteoclastogenesis positively, due to the expression of several osteoclastogenic molecules. 21 Thus, in the present work, PBMC were selected to address the effect of HAnp on osteoclastogenesis.…”

Section: Discussionmentioning

confidence: 97%

“…21 This study suggested that although osteoclast precursors reside in the CD14+ population, other populations present in PBMC have the ability to modulate osteoclastogenesis positively, due to the expression of several osteoclastogenic molecules. 21 Thus, in the present work, PBMC were selected to address the effect of HAnp on osteoclastogenesis.…”

Section: Discussionmentioning

confidence: 97%

“…47 Regarding this, in the present work, the effects of HA nanoparticles were assessed in PBMC, which comprises a mixed population of monocytes (containing the subset of CD14+ osteoclast precursors), lymphocytes and granulocytes, 48 and a previous study showed that the CD14− population is able to express several osteoclastogenic molecules such as M-CSF, RANKL, TNF-, IL-1 , IL-6, IL-17 and GM-CSF, which have the ability to modulate osteoclastogenesis positively. 21 Thus, it might be hypothesized that, in the present protocol, HAnp may affect the expression of these molecules by PBMC and/or the ability of osteoclastic cells to sense and react to the presence of such molecules, favoring the commitment of osteoclast precursors towards osteoclastic differentiation. This characterization is presently underway.…”

Section: Discussionmentioning

confidence: 99%

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Complex Effect of Hydroxyapatite Nanoparticles on the Differentiation and Functional Activity of Human Pre-Osteoclastic Cells

Costa‐Rodrigues¹,

Silva²,

Santos³

et al. 2014

j biomed nanotechnol

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Nanosized hydroxyapatite (HA) is a promising material in clinical applications targeting the bone tissue. NanoHA is able to modulate bone cellular events, which accounts for its potential utility, but also raises safety concerns regarding the maintenance of the bone homeostasis. This work analyses the effects of HA nanoparticles (HAnp) on osteoclastic differentiation and activity, an issue that has been barely addressed. Rod-like HAnp, produced by a hydrothermal precipitation method, were tested on peripheral blood mononuclear cells (PBMC), which contains the CD14+ osteoclastic precursors, in unstimulated or osteoclastogenic-induced conditions. HAnp were added at three time-points during the osteoclastic differentiation pathway, and cell response was evaluated for osteoclastic related parameters. Results showed that HAnp modulated the differentiation and function of osteoclastic cells in a dose-and time-dependent manner. In addition, the effects were dependent on the stage of osteoclastic differentiation. In unstimulated PBMC, HAnp significantly increased osteoclastogenesis, leading to the formation of mature osteoclasts, as evident by the significant increase of TRAP activity, number of TRAP-positive multinucleated cells, osteoclastic gene expression and resorbing ability. However, in a population of mature osteoclasts (formed in osteoclastogenic-induced PBMC cultures), HAnp caused a dose-dependent decrease on the osteoclastic-related parameters. These results highlight the complex effects of HAnp in osteoclastic differentiation and activity, and suggest the possibility of HAnp to modulate/disrupt osteoclastic behavior, with eventual imbalances in the bone metabolism. This should be carefully considered in bone-related and other established and prospective biomedical applications of HAnp.

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Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14+ and CD14− cell fractions

Abstract: Results show that although osteoclastic precursors reside in the CD14(+) cell subpopulation, other populations (such as CD14(-) cells) derived from PBMC, have the ability to modulate osteoclastogenesis positively.

Cited by 31 publications

References 50 publications

Variability of the paracrine‐induced osteoclastogenesis by human breast cancer cell lines

Variability of the paracrine‐induced osteoclastogenesis by human breast cancer cell lines

The multi-differentiation potential of peripheral blood mononuclear cells

Complex Effect of Hydroxyapatite Nanoparticles on the Differentiation and Functional Activity of Human Pre-Osteoclastic Cells

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