CD34+ fibrocytes in neoplastic and inflammatory pancreatic lesions

Barth, Peter; Ebrahimsade, Schokufe; Hellinger, A.; Moll, Roland; Ramaswamy, Annette

doi:10.1007/s00428-001-0551-3

Cited by 87 publications

(71 citation statements)

References 20 publications

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“…Already in 1994, Bucala et al [25] have identified a distinct leukocyte subpopulation, termed fibrocytes, exhibiting characteristics of both stem cells and myo-/fibroblasts. Various studies have confirmed the contribution of circulating fibrocytes to wound repair and fibrosis [26,27]. Mesenchymal stem cells represent a further BM-derived population with multilineage potential and their transition into myofibroblasts has been shown [24,28].…”

Section: Discussionmentioning

confidence: 94%

Bone marrow-derived pancreatic stellate cells in rats

Sparmann

Kruse²,

Hofmeister-Mielke

et al. 2010

Cell Res

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Section: Discussionmentioning

confidence: 94%

Bone marrow-derived pancreatic stellate cells in rats

Sparmann

Kruse²,

Hofmeister-Mielke

et al. 2010

Cell Res

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“…An interesting finding was the coexpression of CD34, CD117 and bcl-2 in stromal cells. CD34, a myeloid stem cell marker, is known to be expressed by fibrocytes in neoplastic and inflammatory pancreatic lesions 6 and seems to play an important role in maintaining stromal integrity and inhibition of tumor cell migration. 7 It is also observed in solitary fibrous tumors, which may occur in the pancreas.…”

Section: Resultsmentioning

confidence: 99%

Cellular hamartoma resembling gastrointestinal stromal tumor: a solid tumor of the pancreas expressing c-kit (CD117)

et al. 2005

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Solid tumors of the pancreas are usually neoplastic. We report on two adult patients, each with a solid tumor of the pancreas that presented with an unusual histology and seemed to follow a benign course. The tumors, one located in the body and one in the tail, were well demarcated and composed of irregularly arranged but welldifferentiated acini and small intralobular and interlobular ducts embedded in predominantly hypocellular fibrotic tissue that contained fascicles of cytologically bland spindle cells. Islets were lacking, but immunohistochemical staining for chromogranin A and insulin revealed individual scattered insulin-producing cells distributed between acinar and ductal cells. The spindle cell component tissue showed coexpression of CD34, c-kit (CD117) and bcl-2. The follow-up (2 and 4 years) of the patients was uneventful. We propose to designate the tumors as 'cellular hamartoma resembling gastrointestinal stromal tumor.' Modern Pathology ( Keywords: pancreatic tumor; hamartoma; c-kit; CD34; bcl-2; differential diagnosis Well-demarcated tumor-like lesions in the pancreas are uncommon. They have been described under the term pseudotumor 1 or inflammatory pseudotumor. 2 Recently, we described a tumorous lesion that we termed pancreatic solid and cystic hamartoma. 3 Here we report on two further solid pancreatic tumors that have features in common with both hamartomas and gastrointestinal stromal tumors. Patients, materials and methodsThe cases were collected from the consultation files of the Department of Pathology of the University of Kiel, to which they had been submitted by the Department of Pathology in Dinslaken, Germany (case 1), and the Department of Pathology of the University of Coimbra, Portugal (case 2).In the first case, a 51-year-old man without clinical symptoms had a routine check-up, during which a mass, 3 cm in diameter, was detected by ultrasonography in the tail of the pancreas next to the splenic artery. This finding was confirmed by endosonography and CT. Laboratory tests were normal. As a result of the unclear nature of the tumor, abdominal surgery was performed with resection of the tumor.In the second case, a 54-year-old woman with slight abdominal discomfort was examined by ultrasonography, which revealed a well-demarcated tumor, 2 cm in diameter, in the body of the pancreas. A left-sided pancreatic resection was performed to remove the tumor. Both patients had an uncomplicated postoperative course and so far (2 and 4 years, respectively) have relapse-free follow-up.Representative 4 mm sections of formalin-fixed, paraffin-embedded tissue from both specimens were stained with hematoxylin and eosin (H&E) and periodic acid-Schiff. Immunohistochemical staining was performed using the standard avidinbiotin method against following antibodies: cytokeratin 8 (CAM 5

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“…+ fibrocytes (CD34 is a 110 kDa surface antigen selectively expressed on human haematopoietic progenitor cells) originating from myeloid precursors in the bone marrow were considered as a source of myofibroblasts in cancer of the colon [129], pancreas, [130], and breast [131]. Experimental studies have shown that CD34 + fibrocytes are attracted by the secondary lymphoid chemokine CCL-21, a ligand of the CCR7 chemokine receptor, and invade sites of tissue damage in vivo.…”

Section: The Origin Of Myofibroblastsmentioning

confidence: 99%