CD34+ cell count predicts long lasting life in the oldest old

Mandraffino, Giuseppe; Aragona, Caterina Oriana; Basile, Giorgio; Cairo, V.; Mamone, F.; Morace, Carmela; D’Ascola, Angela; Alibrandi, Angela; Gullo, Alberto Lo; Loddo, Saverio; Saitta, Antonino; Imbalzano, Egidio

doi:10.1016/j.mad.2017.03.003

Cited by 15 publications

(14 citation statements)

References 52 publications

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“…Patients undergone a complete clinical examination during the clinical appointment, including DAS28-CRP calculation. In PsA patients, cutaneous lesions were examined and the Psoriasis Area and Severity Index (PASI) was calculated to assess the severity [29]. The cut-off for low disease activity was established in 2.9 according to the literature [30].…”

Section: Subjectsmentioning

confidence: 99%

“…Flow cytometry was used for the quantification of CD34+ frequency in peripheral blood samples as previously described [25,29]. Briefly, 50 µL of peripheral blood was incubated with 10 µL of PEconjugated anti-human CD34 antibody (BD) in TRUCOUNT tubes (BD) for 15 minutes.…”

Section: Cd34+ Cell Countmentioning

confidence: 99%

“…cells that expressed the stem cell antigen CD34 were defined as progenitor haematopoietic CD34+ cells, and estimated and counted (cells/µL) as absolute count as previously described [25,29].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Arterial stiffness improvement after adding on PCSK9 inhibitors or ezetimibe to high-intensity statins in patients with familial hypercholesterolemia: A Two–Lipid Center Real-World Experience

Mandraffino

Purrello

Rodríguez‐Carrio

et al. 2020

Journal of Clinical Lipidology

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Background and aims: Cardiovascular (CV) morbidity is increased in inflammatory joint diseases (IJD), as rheumatoid (RA) and psoriatic arthritis (PsA). Whereas increased prevalence of subclinical atherosclerosis has been reported in these conditions, whether an early myocardial functionality is also impaired remains unknown. The aim of this study was to evaluate the myocardial functionality by speckle-tracking echocardiography (STE) in recent onset RA and PsA patients and its potential associations with the levels of circulating CD34+ cells, vitamin D, and with disease activity.Methods: STE was used to assess the myocardial functionality in patients with very early RA (n=41) and PsA (n=35) without traditional CV risk factors, and 58 matched healthy controls (HC). Global longitudinal and circumferential strain (GLS and GCS) were estimated. Pulse wave velocity (PWV), carotid intimamedia thickness (cIMT) were measured as surrogate markers of atherosclerosis. Circulating CD34+ counts were evaluated by flow cytometry and vitamin D levels were quantified by HPLC. Disease activity was assessed by Disease Activity Score-28 (DAS28).Results: RA patients exhibited impaired GLS and GCS (both p<0.001) as compared to HC, GLS being also altered in PsA (p=0.020 vs. HC). DAS28 was correlated to GLS (r=0.908, p<0.001) and GCS (r=0.868, p<0.001) in RA, these findings being confirmed by multivariate regression analyses adjusted for confounders and Principal Component Analyses. GLS and GCS were impaired in PsA patients with high disease activity as compared to HC, and GLS was found to be a predictor of cIMT in this condition. On the other hand, vitamin D was negatively associated with cIMT in HC (r=-0.308, p=0.026) but not in PsA or RA, although decreased levels were observed (both p<0.001). Vitamin D was an independent predictor of decreased CD34+ levels in PsA and RA. CD34+ counts negatively correlated DAS28, GLS and GCS in RA.Conclusions: Subclinical myocardial dysfunction is observed in IJD patients with preserved left-ventricular function and without traditional CV risk factors. Subclinical myocardial dysfunction was found to be a very early event in IJD. Disease activity was the main predictor of myocardial strain impairment. Interestingly, myocardial function was altered and associated with cIMT also in PsA patients with high disease activity.

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Section: Subjectsmentioning

confidence: 99%

Section: Cd34+ Cell Countmentioning

confidence: 99%

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Arterial stiffness improvement after adding on PCSK9 inhibitors or ezetimibe to high-intensity statins in patients with familial hypercholesterolemia: A Two–Lipid Center Real-World Experience

Mandraffino

Purrello

Rodríguez‐Carrio

et al. 2020

Journal of Clinical Lipidology

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“…[ 54 ] In the oldest old (>80 years), the number of circulating hematopoietic stem and progenitor cells (CD34+ cell count) are better predictors of lifespan and cardiovascular disease‐related mortality as compared to classic cardiovascular risk factors. [ 55 ] Mutations naturally occurring in blood cells during a lifetime and revealed by whole‐genome sequencing, can be used as a barcode for tracking and quantifying adult stem cells and their offsprings in the bloodstream. This technique has been applied to demonstrate that all peripheral white blood cells of a 115‐year‐old woman were the offspring of only two hematopoietic stem cell clones [ 56 ] suggesting that the diversity of active stem cells is significantly depleted at this advanced age.…”

Section: Molecular and Cellular Biomarkersmentioning

confidence: 99%

Aging Biomarkers: From Functional Tests to Multi‐Omics Approaches

Kudryashova¹,

Burka²,

Kulaga

et al. 2020

Proteomics

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Aging results in various deleterious changes in the human body that may lead to loss of function and the manifestation of chronic diseases. While diseases can generally be reliably diagnosed, the aging process itself requires more sophisticated approaches to evaluate its progression. Numerous attempts have been made to establish biomarkers to quantify human aging at the cellular, tissue, and organismal level. Here, an up‐to‐date overview of biomarkers related to human aging with an emphasis on biomarkers that take into account different mechanisms of aging between individuals is provided. Classical discrete molecular and non‐molecular biomarkers handpicked by researches on the base of their strong correlation with age, as well as emerging omics‐based biomarkers, are discussed and potential future directions and developments in the field of aging assessment are outlined.

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“…For instance, a decline in CD34+ circulating progenitor cells was reported with advancing age. When 100 octogenarians were observed for 7 and 10 years it was demonstrated that the number of their circulating CD34+ cells better predicted their lifespan and cardiovascular (CV) issues related mortality then the classic cardiovascular risk factors (hypertension, smoking, hypercholesterolemia), levels of inflammatory markers, or levels of cholesterol, or some other traditional cardiovascular indexes such as FRS and CVFRs The chances of reaching an older age depended on higher numbers of CD34+ cells at baseline, thus the number of CD34+ cells could be considered as a biomarker of longevity in the elderly over 80 years [235].…”

Section: Stem Cell Exhaustionmentioning

confidence: 99%

Stem Cell Aging

Rožman¹,

Jazbec²,

Jež³

2018

Stem Cells in Clinical Practice and Tissue Engineering

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Stem cells persist throughout life, replacing cells lost to homeostatic turnover, injury, and disease. However, their functions decline with age, which contributes to degeneration and dysfunction. The molecular mechanisms involved in the aging of stem cells are the same as the ones involved in the aging of somatic cells, including telomere shortening, oxidative stress, epigenetic dysregulation, miRNAs changes, alterations of DNA, RNA, proteome, and various cellular organelles. Aging impacts various pathways, such as insulin/insulin-like growth factor 1 (IGF-1), mTOR, FoxO, AMP-activated protein kinase (AMPK), sirtuin, and many others, resulting in senescent stem cells that exhibit functional and numerical impairment. Stem cells have developed special mechanisms to prevent age related damage accumulation and to sustain their stemness properties, however, these mechanisms lose their effectiveness over time. The most fatal consequence of this is found in the immune system, where both innate and adaptive immunity are affected, exhibiting a plethora of defects, including increased autoimmune disease occurrence, elevated tolerance to cancer and chronic inflammatory status. Stem cell therapies call for the best quality of stem cells grafts. Stem cell products should be devoid of cells containing a senescent phenotype, thus a comprehensive knowledge of the biology behind the senescence of stem cells should be taken into account in every cell based therapy.

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CD34+ cell count predicts long lasting life in the oldest old

Cited by 15 publications

References 52 publications

Arterial stiffness improvement after adding on PCSK9 inhibitors or ezetimibe to high-intensity statins in patients with familial hypercholesterolemia: A Two–Lipid Center Real-World Experience

Arterial stiffness improvement after adding on PCSK9 inhibitors or ezetimibe to high-intensity statins in patients with familial hypercholesterolemia: A Two–Lipid Center Real-World Experience

Aging Biomarkers: From Functional Tests to Multi‐Omics Approaches

Stem Cell Aging

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