CD26: A negative selection marker for human Treg cells

Salgado, Francisco; Pérez-Díaz, Amparo; Villanueva, Nora M.; Lamas, Olaya; Arias, Pilar; Nogueira, Marco Aurélio

doi:10.1002/cyto.a.22117

Cited by 46 publications

(38 citation statements)

References 61 publications

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“…The ability to produce IL-10 is strongly associated with the combinatorial expression of CD39 and CD26, with the CD39 2 CD26 + Treg cells showing the highest production of IL-10. Unlike the indication of CD26 as a negative selection marker for Treg cells (41,42), our CD39 2 CD26 + Treg cells are high in FOXP3 expression (data not shown), and they exert the same level of suppressive activity as the CD39 + CD26 2 Treg cells (Fig. 2E) due to our CD45RO high gating strategy.…”

Section: Discussionmentioning

confidence: 54%

Diverse Gene Expression in Human Regulatory T Cell Subsets Uncovers Connection between Regulatory T Cell Genes and Suppressive Function

Hua

Davis

Hill

et al. 2015

The Journal of Immunology

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Regulatory T (Treg) cells have a critical role in the control of immunity, and their diverse subpopulations may allow adaptation to different types of immune responses. In this study, we analyzed human Treg cell subpopulations in the peripheral blood by performing genome-wide expression profiling of 40 Treg cell subsets from healthy donors. We found that the human peripheral blood Treg cell population is comprised of five major genomic subgroups, represented by 16 tractable subsets with a particular cell surface phenotype. These subsets possess a range of suppressive function and cytokine secretion and can exert a genomic footprint on target effector T (Teff) cells. Correlation analysis of variability in gene expression in the subsets identified several cell surface molecules associated with Treg suppressive function, and pharmacological interrogation revealed a set of genes having causative effect. The five genomic subgroups of Treg cells imposed a preserved pattern of gene expression on Teff cells, with a varying degree of genes being suppressed or induced. Notably, there was a cluster of genes induced by Treg cells that bolstered an autoinhibitory effect in Teff cells, and this induction appears to be governed by a different set of genes than ones involved in counteracting Teff activation. Our work shows an example of exploiting the diversity within human Treg cell subpopulations to dissect Treg cell biology.

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Section: Discussionmentioning

confidence: 54%

Diverse Gene Expression in Human Regulatory T Cell Subsets Uncovers Connection between Regulatory T Cell Genes and Suppressive Function

Hua

Davis

Hill

et al. 2015

The Journal of Immunology

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“…Fresh CD8 T cells were derived from unstimulated, freshly isolated PBMCs and were purified with a CD8 T Cell Isolation Kit (Miltenyi Biotec). To isolate CD8 Tregs directly without culture, CD8 T cells were purified from PBMCs using a CD8 T Cell Isolation Kit (Stemcell Technologies) and stained with FITC-labeled CD39 plus PE-Cy7-labeled CD26 (Biolegend) (23,24). CD8 + CD39 + CD26 -cells were sorted with a BD FACSAria cell sorter.…”

Section: Methodsmentioning

confidence: 99%

NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

Wen

Shimojima

Shirai

et al. 2016

Journal of Clinical Investigation

113

110

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“…The absence of CD26, a dipeptidyl peptidase IV, from the surface of CD39 + Treg can further facilitate their isolation and purity by concomitant removal of CD4 + CD26 + T cells, as we recently reported [49]. A method for Treg isolation based on the depletion of CD4 + CD26 + T cells has been published by another group as well [50]. Importantly, CD26 is an anchor protein for adenosine deaminase (ADA), an enzyme responsible for hydrolysis of adenosine to inosine [49].…”

Section: Phenotypic Characteristics Of Human Tregmentioning

confidence: 97%

Induced and natural regulatory T cells in human cancer

Whiteside¹,

Schuler²,

Schilling³

2012

Expert Opinion on Biological Therapy

111

102

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Introduction Evidence suggests that FOXP3+CD25highCD4+ regulatory T cells (Treg) which accumulate in cancer may have beneficial or unfavorable effects on prognosis. The presence in tumor-associated inflammatory infiltrates of two subsets of Treg with distinct phenotypic and functional profiles might explain these conflicting observations. Areas covered Human inducible (i) Treg arising by tumor-driven conversion of conventional CD4+ T cells are highly suppressive, therapy-resistant Treg which down-regulate anti-tumor immune responses, promoting tumor growth. Natural (n) Treg, normally responsible for maintaining peripheral tolerance, control cancer-associated inflammation, which favors tumor progression. This division of labor between nTreg and iTreg is not absolute, and overlap may be common. Nevertheless, iTreg play a critical and major role in cancer and cancer therapy. The tumor microenvironment determines the type, frequency and suppression levels of accumulating Treg. Expert opinion In cancer, a selective removal or silencing of iTreg and not of nTreg should be a therapeutic goal. However, the implementation of this challenging strategy requires further studies of cellular and molecular crosstalk among immune cells in the tumor microenvironment.

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CD26: A negative selection marker for human Treg cells

Cited by 46 publications

References 61 publications

Diverse Gene Expression in Human Regulatory T Cell Subsets Uncovers Connection between Regulatory T Cell Genes and Suppressive Function

Diverse Gene Expression in Human Regulatory T Cell Subsets Uncovers Connection between Regulatory T Cell Genes and Suppressive Function

NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

Induced and natural regulatory T cells in human cancer

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