CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma Cells

Abstract

Owing to lack of appropriate model systems, investigations of meningioma biology have come to a stop. In this study, we developed a comprehensive digestion method and defined a culture system. Using this method and system, primary meningioma cells in conditioned suspension medium and a hypoxic environment could be amplified in spheres and were passaged for more than ten generations. Meningioma sphere cells were positive for meningioma cell markers and negative for markers of neural cell types. Importantly, we found the cells expressed the stem cell marker, CD133, but not nestin. All of the tumor sphere cell populations showed a slower degree of cell proliferation than that of human glioma cells and fetal neural stem cells (NSCs). Further studies showed that the proliferative rate was positively correlated with CD133 expression. The higher the CD133 expression, the faster the cell proliferation. With the increase in cell generations, the cell proliferation rate gradually slowed down, and CD133 expression also decreased. Single CD133+ cells rather than CD133− cells could form spheres. Thus, the results above indicated that those cells expressing CD133 in spheres might be stem-like cells, which may be responsible for efficient amplification of human meningioma cells. Decreased expression of CD133 may lead to the failure of long-term passaging.

VEGF Promotes Proliferation of Human Glioblastoma Multiforme Stem‐Like Cells through VEGF Receptor 2

“…Cells were then cultured in stem cell culture medium for at least 4 hours to recover surface antigens. Cells were then labeled with APC- or PE-conjugated CD133 antibody and sorted by fluorescence-activated cell sorting (FACS) as described previously [33]. CD133-positive cells were designated as GBSCs, which were resuspended in serum-free DMEM/F-12 containing human recombinant N 2 (20 ng/mL; Invitrogen), EGF (20 ng/mL; Invitrogen), and bFGF (20 ng/mL; Gibco) and then seeded in a nonadherent cell culture flask (5 mL per flask) at a density of 5 × 10 6 live cells per flask.…”

“…Culture media was changed twice a week, and neurospheres are passaged every 1 or 2 weeks, depending on the growth rate of each sample. Immunofluorescence staining to detect the expression of brain tumor stem cell markers was performed as described previously [33]. …”

Expression of nestin, cd133 and sox2 in meningiomas

“…Therefore, we presume that meningioma tumor stem cells may be responsible for these events. According to recent reports, CSC-like cells may be present in meningiomas (13,17,30). Traditionally, Nestin, CD133 and Sox2 are regarded as CSC markers, but they could also be considered specific markers for BTSCs (28).…”

“…According to Singh et al, 100 CD133+ cells are sufficient to initiate brain tumors in non-obese diabetic (NOD)/severe combined immunodeficient (SCID) mice, whereas 100,000 CD133- (5,6,14,18,19,23,31). Recently, Tang et al successfully isolated meningioma cells from six patients and showed that CD133 expression levels were related to cell proliferation rates (30). Therefore, CD133 is regarded as a specific marker of BTSCs in malignant brain tumors (26).…”

Multiple Meningiomas Characterized by Benign and Malignant Tumor Entities

“…Many tumors have shown to display gene expression signatures characteristic of human embryonic stem cells [8]. There may be stemlike cells in meningiomas [1,9]. Prakash Rath [1] had succeeded in isolating meningioma-initiating cells from atypical meningioma.…”