CD133+adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF)

Abstract

Background: CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres.

Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

“…& CD 133 (Prominin-1) (monoclonal mouse IG1, CD133/1, (AC 133, pure, Miltenyi, Biotec, 130-090-422, dilution 1/10) is a pentaspan protein membrane stem cell marker, specific to primitive human, mammalian, and mice hematopoietic and neural progenitor (PG) cells [23,24]. CD133+ cells are considered a quiescent PG cell population in the adult brain [25] and in the adult postmortem retina [26]. We have reported that CD133 is expressed at the external limiting membrane (ELM) level in the human fetal retina and in the adult human retina (Ducournau Y, Boscher C, Ducournau D, Guillaubey C, Mosnier JF.…”

“…It has been reported that some of these cells can be dormant or slow-cycling, and suggested that CD133 might be down-regulated during G0/G1 [32].Therefore, as we did observe CE rhodopsin expression, we cannot rule out the possibility of missing a period of transitory CD133 expression. Also, recent findings on the commitment of CD133+ retinal cells towards differentiation show that the process might be promoted by the cytokine leukaemia inhibitory factor (LIF), which is endogenously secreted in cases of neural tissue injury or damage [26]. The framework of the disease RD with PVR might be assimilated to damage, and might have promoted the differentiation of CE CD133+ cells into PR and negatived CD133 staining.…”

“…6) suggests that in RD with PVR, either progenitor cells can be generated in the retina itself, or photoreceptor reorganization can be stimulated. Authors using progenitor cell markers other [26], and might stimulate further attention for this marker as a retinal one. CD133 positive staining at the level of the ELM and of the photoreceptors in the retina of the control eye is difficult to interpret; the eye itself was indeed normal, and there was no exudative RD accompanying the orbital tumor.…”

“…CD133 positive staining at the level of the ELM and of the photoreceptors in the retina of the control eye is difficult to interpret; the eye itself was indeed normal, and there was no exudative RD accompanying the orbital tumor. One cannot rule out some reactional process to ocular compression by the tumor; most of all, CD133 cell expression signification is far from being completely elucidated [26].…”

c-Kit+ cells isolated from human fetal retinas represent a new population of retinal progenitor cells

“…The continuous development of flow cytometry techniques to assess cell surface antigens has provided biomarkers that can be used to obtain highly purified tissue-specific RPCs. To date, several cell markers have proven to be suitable for the specific identification, isolation and enrichment of RPCs (Carter et al, 2009;Koso et al, 2009); these markers are invaluable for RPC research.…”