Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

Ducournau, Yvette; Boscher, C; Adelman, Ron A.; Guillaubey, Colette; Schmidt-Morand, Didier; Mosnier, Jean‐François; Ducournau, D

doi:10.1007/s00417-011-1797-3

Cited by 15 publications

(12 citation statements)

References 50 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, hyperplasia of the CB, forming "neurosphere-like" structures were found. There were no GFAP+ cells, but unlike in our study, a few rhodopsin+ cells were found in the vicinity of the CE (106). Further analysis of more eyes with retinal damage may be needed to clarify the exact expression patterns.…”

Section: Does the Adult Human Cb Harbor Stem Cells?contrasting

confidence: 83%

A comparison of epithelial and neural properties in progenitor cells derived from the adult human ciliary body and brain

Moe

Kolberg

Sandberg

et al. 2009

Experimental Eye Research

View full text Add to dashboard Cite

Section: Does the Adult Human Cb Harbor Stem Cells?contrasting

confidence: 83%

A comparison of epithelial and neural properties in progenitor cells derived from the adult human ciliary body and brain

Moe

Kolberg

Sandberg

et al. 2009

Experimental Eye Research

View full text Add to dashboard Cite

“…Therefore, EGF is considered a major factor in the development of PVR [8,11]. In this study, we found that EGF-dependent proliferation and migration in RPE cell were inhibited by 1.0 μM AF (Fig 5).…”

Section: Discussionsupporting

confidence: 51%

“…Several groups have already indicated that EGF enhances RPE cell survival and can stimulate proliferation and migration of RPE cells through activation of the EGFR signaling pathway [6–8]. Some studies show that the activation of EGF seems to be an important factor of the pathogenesis of PVR [9–11]. …”

Section: Introductionmentioning

confidence: 99%

Auranofin Inhibits Retinal Pigment Epithelium Cell Survival through Reactive Oxygen Species-Dependent Epidermal Growth Factor Receptor/ Mitogen-Activated Protein Kinase Signaling Pathway

Chen

Tzekov

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Abnormal survival of retinal pigment epithelium (RPE) cells contributes to the pathogenesis of proliferative vitreoretinopathy (PVR), a sight-threatening disease. In this study, we explored the effect of the anti-rheumatic agent auranofin (AF) on RPE cell survival and studied the underlying signaling mechanisms in vitro. Our results showed that AF inhibited ARPE-19 cell survival in a dose and time-dependent manner. Application of AF induced several effects: a significant decrease in total epidermal growth factor receptor (EGFR) and an increase in phosphorylated EGFR and mitogen-activated protein kinase (MAPK), including extracellular signal-regulated kinase (ERK), P38 mitogen-activated protein kinase (P38MAPK), c-Jun N-terminal kinase (JNK), c-Jun, mitogen activated protein kinase activated protein kinase 2(MAPKAPK2), and heat shock protein 27 (HSP27). AF also inhibited epidermal growth factor (EGF)-dependent cell proliferation and migration through affecting EGFR/MAPK signaling. The antioxidant N-acetylcysteine (NAC) blocked the AF-induced increase of reactive oxygen species (ROS) production, the reduction of total EGFR, and the phosphorylation of multiple nodes in EGFR/MAPK signaling pathway. P38MAPK inhibitor SB203580, but not inhibitors of EGFR (erlotinib), ERK (FR180204) and JNK (SP600125), suppressed AF-induced phosphorylation of EGFR/p38MAPK/MAPKAPK2/Hsp27. In conclusion, the ROS-dependent phosphorylation of EGFR/MAPK is an important signaling pathway for AF-induced inhibition of RPE cell survival, and AF may have the potential for treatment of abnormal survival of RPE cells in PVR.

show abstract

“…The vitreous was extracted with a 5-mL syringe, following the aforementioned steps at different time points (3,7,14,21, and 28 days). The test was processed according to instructions provided in the ELISA kit (LifeSpan Biosciences, Seattle, WA).…”

Section: Detection Of Egf Content In the Vitreousmentioning

confidence: 99%

Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Ren

Zhao

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Proliferative vitreoretinopathy (PVR) is a common refractory eye disease that causes blindness and occurs after retinal detachment or retinal reattachment. Epidermal growth factor (EGF) has been shown to play an important role in the migration and proliferation of retinal pigment epithelium (RPE) cells, which promote PVR. Curcumin inhibits RPE cell proliferation, but it is not known whether it participates in the formation of PVR. Curcumin regulates the biological functions of EGF, which plays important roles in the development of PVR. This study aimed to evaluate the effect of curcumin on the regulation of EGF in PVR. Methods: Rabbit RPE cells were cultured, and EGF expression was detected by immunocytochemistry. MTT assay was conducted to determine cell proliferation induced by different concentrations of EGF. Immunocytochemical staining was used to detect EGF expression after treatment with curcumin at varying concentrations. Real-time PCR (RT-PCR) and western blot analysis were used to detect the concentrations of EGF mRNA and protein after treatment with curcumin. After RPE cells and curcumin were injected into experimental rabbit eyes, the cornea, aqueous humor, lens, and vitreous opacity were observed and recorded simultaneously by indirect ophthalmoscopy, fundus color photography, and B-ultrasonography. The vitreous body was extracted, and the EGF content in the vitreous humor was measured by enzyme-linked immunosorbent assay (ELISA). Results: At each time point (24, 48, and 72 h), cell proliferation gradually increased with increasing EGF concentrations (0, 3, 6, and 9 ng/mL) in a dose-dependent manner. Cell proliferation between EGF concentrations of 9 and 12 ng/mL were no different, which suggested that 9 ng/mL EGF was the best concentration to use to stimulate RPE cell proliferation in vitro. Under all EGF concentrations (0, 3, 6, 9, and 12 ng/mL), RPE cell proliferation increased with time (from 24 to 72 h), suggesting a time–effect relationship. Curcumin downregulated EGF expression in RPE cells, which also indicated time–effect and dose–effect relationships. The best curcumin concentration for the inhibition of EGF expression was 15 µg/mL. RT-PCR and western blot analyses indicated that the EGF mRNA and expression of the protein in RPE cells treated with curcumin significantly decreased with time. Ocular examinations revealed that the vitreous opacity was lower and the proliferative membrane was thinner in the curcumin group compared with the control group. The PVR grade and the incidence of retinal detachment were significantly lower in the experimental group than in the control group. ELISA results showed that the EGF content in vitreous humor was higher in the control group than in the curcumin group. The curcumin and control groups were significantly different at each time point. Conclusion: Curcumin inhibited RPE cell proliferation by downregulating EGF and thus effectively inhibited the initiation and development of PVR.

show abstract

Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

Cited by 15 publications

References 50 publications

A comparison of epithelial and neural properties in progenitor cells derived from the adult human ciliary body and brain

A comparison of epithelial and neural properties in progenitor cells derived from the adult human ciliary body and brain

Auranofin Inhibits Retinal Pigment Epithelium Cell Survival through Reactive Oxygen Species-Dependent Epidermal Growth Factor Receptor/ Mitogen-Activated Protein Kinase Signaling Pathway

Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Contact Info

Product

Resources

About