CD13 in cell adhesion: aminopeptidase N (CD13) mediates homotypic aggregation of monocytic cells

Mina‐Osorio, Paola; Shapiro, Linda H.; Ortega, Enrique

doi:10.1189/jlb.0705425

Cited by 56 publications

(120 citation statements)

References 40 publications

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“…The same study also demonstrates that inhibitors of tyrosine kinases and phosphoinositide 3-kinase block the increase in calcium influx as well as the phosphorylation of MAPKs in the presence of the mAb against APN (Santos et al, 2000). In addition, antibodies to APN that have little effect on enzymatic activity exhibit a high capacity to induce cell adhesion in monocytes; this effect can be inhibited by SB203580, a p38 MAPK inhibitor (Mina-Osorio et al, 2006). APN has a very short cytoplasmic domain without any known signaling motifs (Luan and Xu, 2007) The co-distribution of SFN with APN at the leading edge of fibroblasts was unpredicted.…”

Section: Discussionmentioning

confidence: 76%

14-3-3σ associates with cell surface aminopeptidase N in the regulation of matrix metalloproteinase-1

Ghaffari

Kilani

et al. 2010

Journal of Cell Science

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SummaryMatrix metalloproteinases (MMPs) are implicated in the degradation of the extracellular matrix during development and tissue repair, as well as in pathological conditions such as tumor invasion and fibrosis. MMP expression by stromal cells is partly regulated by signals from the neighboring epithelial cells. Keratinocyte-releasable 14-3-3, or stratifin, acts as a potent MMP-1-stimulatory factor in fibroblasts. However, its mechanism of transmembrane signaling remains unknown. Ectodomain biotin labeling, serial affinity purification and mass spectroscopy analysis revealed that the stratifin associates with aminopeptidase N (APN), or CD13, at the cell surface. The transient knockdown of APN in fibroblasts eliminated the stratifin-mediated p38 MAP kinase activation and MMP-1 expression, implicating APN in a receptor-mediated transmembrane signaling event. Stratifin deletion studies implicated its C-terminus as a potential APN-binding site. Furthermore, the dephosphorylation of APN ectodomains reduced its binding affinity to the stratifin. The presence of a phosphorylated serine or threonine residue in APN has been implicated. Together, these findings provide evidence that APN is a novel cell surface receptor for stratifin and a potential target in the regulation of MMP-1 expression in epithelial-stromal cell communication.

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Section: Discussionmentioning

confidence: 76%

14-3-3σ associates with cell surface aminopeptidase N in the regulation of matrix metalloproteinase-1

Ghaffari

Kilani

et al. 2010

Journal of Cell Science

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“…It was also reported that CD13 could block the apoptosis induction by tumor necrosis factor-alpha (26) and enhance sensitivity of tumor cells to cisplatin (27). CD13 was also proved to be involved in cell adhesion (28) as well as in endothelial invasion (29). Recent study made by Haraguchi and colleagues proved that CD13 may be a therapeutic target in human liver cancer stem cells and inhibition of CD13 by monoclonal antibody could induce cell apoptosis (30).…”

Section: Discussionmentioning

confidence: 99%

Integrin α6high Cell Population Functions as an Initiator in Tumorigenesis and Relapse of Human Liposarcoma

Wang

et al. 2011

Molecular Cancer Therapeutics

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The relapse and resistance to chemo-and radiotherapy are main problems in the treatment of human liposarcoma. It is important to find a functional marker existing in the liposarcoma cells for targeting. In this article, we established a new sub-cell line SW872-S cells with high tumorigenicity from human liposarcoma SW872 cells by repeated inoculation approach. The characteristic of the sub-cell line is linked to the high levels of integrin a6 on the surface. The integrin a6 high cells show much higher tumor initiation and self-renewal potential in vivo than integrin a6 low cells do. Targeting integrin a6 with its specific short interfering RNA and antibody significantly inhibits the cell adhesion to laminin and the tumor growth in vitro and in vivo, respectively. Interestingly, integrin a6 marks almost all of the surgical biopsy specimens of patients with liposarcoma relapse. Moreover, integrin a6 is found to coexpress with CD13, which might contribute to the antiapoptosis ability of integrin a6 high cells. Consistently, integrin a6 high cells are more sensitive to the CD13 inhibitor bestatin, and 61% of 23 other human tumor cell lines also contain integrin a6 high CD13 high subgroup.These results provide evidence, for the first time, to our knowledge, that integrin a6 and CD13 can serve as functional markers of the tumor-initiation subcell population in human liposarcoma as well as other cancers for therapeutic targeting. Mol Cancer Ther; 10(12); 2276-86. Ó2011 AACR.

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“…CD 29 and CD 44 also are widely (Zuk et al 2002;Gimble et al 2007;Aust et al 2004;Gronthos et al 2001;De Ugarte et al 2003a, b;Katz et al 2005). CD 13, rather than being an important monocyte/ macrophage marker, plays a vital role in angiogenesis and migration (Mins-Osorio et al 2006;Pasqualini et al 2007). It is expressed in higher percentage in SF and moderately expressed in both BM and OF.…”

Section: Discussionmentioning

confidence: 99%

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

Dhanasekaran

Somasundaram²,

Kanmani

et al. 2012

Cytotechnology

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Omentum fat derived stem cells have emerged as an alternative and accessible therapeutic tool in recent years in contrast to the existing persuasive sources of stem cells, bone marrow and subcutaneous adipose tissue. However, there has been a scanty citation on human omentum fat derived stem cells. Furthermore, identification of specific cell surface markers among aforesaid sources is still controversial. In lieu of this existing perplexity, the current research work aims at signifying omentum fat as a ground-breaking source of stem cells by surface antigenic profiling of stem cell population. In this study, we examined and compared the profiling of cell surface antigenic expressions of hematopoietic stem cells, mesenchymal stem cells, cell adhesion molecules and other unique markers such as ABCG2, ALDH and CD 117 in whole cell population of human omentum fat, subcutaneous fat and bone marrow. The phenotypic characterization through flowcytometry revealed the positive expressions of CD 34, CD 45, CD 133, HLADR, CD 90, CD 105, CD 73, CD 29, CD 13, CD 44, CD 54, CD 31, ALDH and CD 117 in all sources. The similarities between the phenotypic expressions of omentum fat derived stem cells to that of subcutaneous fat and bone marrow substantiates that identification of ultimate source for curative therapeutics is arduous to assess. Nevertheless, these results support the potential therapeutic application of omentum fat derived stem cells.

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CD13 in cell adhesion: aminopeptidase N (CD13) mediates homotypic aggregation of monocytic cells

Cited by 56 publications

References 40 publications

14-3-3σ associates with cell surface aminopeptidase N in the regulation of matrix metalloproteinase-1

14-3-3σ associates with cell surface aminopeptidase N in the regulation of matrix metalloproteinase-1

Integrin α6high Cell Population Functions as an Initiator in Tumorigenesis and Relapse of Human Liposarcoma

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

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