Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

Dhanasekaran, M.; Somasundaram, Indumathi; Kanmani, A.; Poojitha, R.; Revathy, K. M.; Rajkumar, J. S.; Sudarsanam, D.

doi:10.1007/s10616-012-9427-4

Cited by 19 publications

(24 citation statements)

References 48 publications

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“…The characteristics of ASCs are closely similar to those of other types of mesenchymal stem cells (MSCs) (Dhanasekaran et al 2012;Zuk et al 2002). They have been successfully tested in preclinical models, which opens a wide field of clinical applications (Wu et al 2011;Zhang et al 2012).…”

Section: Introductionmentioning

confidence: 93%

Harvest site influences the growth properties of adipose derived stem cells

et al. 2012

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The therapeutic potential of adult stem cells may become a relevant option in clinical care in the future. In hand and plastic surgery, cell therapy might be used to enhance nerve regeneration and help surgeons and clinicians to repair debilitating nerve injuries. Adipose-derived stem cells (ASCs) are found in abundant quantities and can be harvested with a low morbidity. In order to define the optimal fat harvest location and detect any potential differences in ASC proliferation properties, we compared biopsies from different anatomical sites (inguinal, flank, pericardiac, omentum, neck) in Sprague-Dawley rats. ASCs were expanded from each biopsy and a proliferation assay using different mitogenic factors, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) was performed. Our results show that when compared with the pericardiac region, cells isolated from the inguinal, flank, omental and neck regions grow significantly better in growth medium alone. bFGF significantly enhanced the growth rate of ASCs isolated from all regions except the omentum. PDGF had minimal effect on ASC proliferation rate but increases the growth of ASCs from the neck region. Analysis of all the data suggests that ASCs from the neck region may be the ideal stem cell sources for tissue engineering approaches for the regeneration of nervous tissue.

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Section: Introductionmentioning

confidence: 93%

Harvest site influences the growth properties of adipose derived stem cells

et al. 2012

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“…The isolations of the stromal vascular fraction (SVF) from the adipose tissue and the mononuclear cells from the bone marrow were performed using our previously reported protocol [21]. The isolated SVF and mononuclear cells were seeded in a culture flask at a seeding density of 3 x 10 4 cell/cm 2 in DMEM -low glucose (DMEM-LG, Invitrogen) with 10% FBS (Invitrogen) and 1% antibiotics (Invitrogen) and incubated at 37ºC, 5% CO 2 in 95% humidity with the media replaced every 3 days until confluence.…”

Section: Isolation and Culturementioning

confidence: 99%

Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro

Dhanasekaran

Somasundaram²,

Dorairaj³

et al. 2013

Cellular and Molecular Biology Letters

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Although stem cells are present in various adult tissues and body fluids, bone marrow has been the most popular source of stem cells for treatment of a wide range of diseases. Recent results for stem cells from adipose tissue have put it in a position to compete for being the leading therapeutic source. The major advantage of these stem cells over their counterparts is their amazing proliferative and differentiation potency. However, their pancreatic lineage transdifferentiation competence was not compared to that for bone marrow-derived stem cells. This study aims to identify an efficient source for transdifferentiation into pancreatic islet-like clusters, which would increase potential application in curative diabetic therapy. The results reveal that mesenchymal stem cells (MSC) derived from bone marrow and subcutaneous adipose tissue can differentiate into pancreatic islet-like clusters, as evidenced by their islet-like morphology, positive dithizone staining and expression of genes such as Nestin, PDX1, Isl 1, Ngn 3, Pax 4 and Insulin. The pancreatic lineage differentiation was further corroborated by positive results in the glucose challenge assay. However, the results indicate that bone marrow-derived MSCs are superior to those from subcutaneous adipose tissue in terms of differentiation into pancreatic islet-like clusters. In conclusion, bone marrow-derived MSC might serve as a better alternative in the treatment of diabetes mellitus than those from adipose tissue.

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“…The mononuclear cell (MNC) population was isolated from UCB according to our previously published protocol (Dhanasekaran et al 2012) (Fig. 1i).…”

Section: Mnc Isolationmentioning

confidence: 99%

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach

et al. 2013

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Human umbilical cord blood (hUCB) has been the preferred source of stem cells for the treatment of haematological malignancies and genetic disorders. This is primarily due to its non-invasiveness, high accessibility with relative ease of isolation. Still failures do prevail due to its heterogeneity and lesser frequency of MSC identified in UCB. This study, thus, employs a cell enrichment technology to improve its therapeutic efficacy. This was achieved by immunophenotypic comparison of stem cells isolated from the heterogenous non-sorted mononuclear cells (MNCs), linage depleted (Lin? and Lin-) fractions obtained from magnetic activated cell sorter (MACS) and sorted MNCs obtained by fluorescent activated cell sorter (FACS). The markers under consideration were CD29, CD44, CD34, CD45, CD133, CD90 and CD117. FACS sorted MNCs were rich in naive stem cell population, whereas non-sorted MNCs and lineage depleted fractions were found to be rich in progenitors. Thus, we suggest that a combination therapy of both sorted population might serve as an alternative valuable tool in treating haematologic/ genetic disorders. However, further research on cell enrichment technology might give a clue for improved cell based therapy in regenerative medicine.

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Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

Cited by 19 publications

References 48 publications

Harvest site influences the growth properties of adipose derived stem cells

Harvest site influences the growth properties of adipose derived stem cells

Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach

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