CD117<sup>+</sup>amniotic fluid stem cells

Cananzi, Mara; Coppi, Paolo De

doi:10.4161/org.22426

Cited by 75 publications

(37 citation statements)

References 106 publications

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“…The clinically cultured AFCs were positive for mesenchymal cell markers and negative for endothelial, hematopoietic and stem cells markers. This observation may be due to the culture medium and state, which is consistent with the results of a previous study on cell phenotypes ( 21 , 22 ). Several studies have derived hematopoietic stem cells from amniotic fluid and performed screening using flow cytometry prior to establishing the primary culture ( 11 ).…”

Section: Discussionsupporting

confidence: 92%

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

Wang¹,

Chen²,

Zhong³

et al. 2014

Experimental and Therapeutic Medicine

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The present study aimed to improve the characterization of amniotic fluid cells (AFCs) in order to optimize their use in chromosomal prenatal diagnosis and as seed or stem cells for tissue engineering. The AFCs used in the current study were obtained from three females in their second trimester of pregnancy. The cells were cultured independently and characterized by cell morphology, cell markers, cell cycle distribution and chromosome Giemsa banding in an early- and late-passage. The AFCs remained homogeneous in culture and expressed mesenchymal markers, but not endothelial markers along the culture process. In addition, compared with the early-passage cells, the late-passage cells exhibit an increase in CD105 expression, a decrease in cell division and a delay in the cell cycle, and a number of cells underwent cell cycle arrest. However, the cells retained a normal karyotype. Therefore, the current study characterized AFCs in a clinical culture and confirmed that AFCs are mesenchymal precursors. The results obtained may be useful for the application of AFCs in prenatal diagnosis.

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Section: Discussionsupporting

confidence: 92%

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

Wang¹,

Chen²,

Zhong³

et al. 2014

Experimental and Therapeutic Medicine

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“…Following these studies, Arnhold et al sorted CD117 positive cells by magnetic associated cell sorting. They indicated that the percentage of CD117 positive cells was 3.2±1.03% of the whole cell population and demonstrated that these cells could differentiate to adipogenic, osteogenic, myogenic and neurogenic lineage ( 12 ). The third one is the two-stage culture protocol established by Tsai et al, using nonadhering AF cells of the primary amniocytes culture to isolate AFSCs.…”

Section: Isolation and Culturing Of Afscsmentioning

confidence: 99%

A Mini Overview of Isolation, Characterization and Application of Amniotic Fluid Stem Cells

Gholizadeh-Ghalehaziz¹,

Farahzadi

Fathi

et al. 2015

IJSC

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Amniotic fluid represents rich sources of stem cells that can be used in treatments for a wide range of diseases. Amniotic fluid- stem cells have properties intermediate between embryonic and adult mesenchymal stem cells which make them particularly attractive for cellular regeneration and tissue engineering. Furthermore, scientists are interested in these cells because they come from the amniotic fluid that is routinely discarded after birth. In this review we give a brief introduction of amniotic fluid followed by a description of the cells present within this fluid and aim to summarize the all existing isolation methods, culturing, characterization and application of these cells. Finally, we elaborate on the differentiation and potential for these cells to promote regeneration of various tissue defects, including fetal tissue, the nervous system, heart, lungs, kidneys, bones, and cartilage in the form of table.

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“…Stem cells in brief are the naïve cells of the body with an exceptional ability to self-renew, proliferate, differentiate, and get programmed for multi-lineage commitment (Cananzi and De Coppi, 2012; Liu et al, 2013; Kumar et al, 2015). Their origin can either be fetal, embryonic, or adult tissues of the body (Nam et al, 2015; Singh et al, 2015).…”

Section: Stem Cells and Induced Pluripotent Stem Cells (Ipscs) In Neumentioning

confidence: 99%

Advances in Stem Cell Research- A Ray of Hope in Better Diagnosis and Prognosis in Neurodegenerative Diseases

Singh

Srivastav

Srivastava

et al. 2016

Front. Mol. Biosci.

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Neurodegeneration and neurodegenerative disorders have been a global health issue affecting the aging population worldwide. Recent advances in stem cell biology have changed the current face of neurodegenerative disease modeling, diagnosis, and transplantation therapeutics. Stem cells also serve the purpose of a simple in-vitro tool for screening therapeutic drugs and chemicals. We present the application of stem cells and induced pluripotent stem cells (iPSCs) in the field of neurodegeneration and address the issues of diagnosis, modeling, and therapeutic transplantation strategies for the most prevalent neurodegenerative disorders. We have discussed the progress made in the last decade and have largely focused on the various applications of stem cells in the neurodegenerative research arena.

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CD117⁺amniotic fluid stem cells

Cited by 75 publications

References 106 publications

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

A Mini Overview of Isolation, Characterization and Application of Amniotic Fluid Stem Cells

Advances in Stem Cell Research- A Ray of Hope in Better Diagnosis and Prognosis in Neurodegenerative Diseases

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CD117+amniotic fluid stem cells

Cited by 75 publications

References 106 publications

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

Levels of CD105+ cells increase and cell proliferation decreases during S-phase arrest of amniotic fluid cells in long-term culture

A Mini Overview of Isolation, Characterization and Application of Amniotic Fluid Stem Cells

Advances in Stem Cell Research- A Ray of Hope in Better Diagnosis and Prognosis in Neurodegenerative Diseases

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Product

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CD117⁺amniotic fluid stem cells