A Mini Overview of Isolation, Characterization and Application of Amniotic Fluid Stem Cells

Gholizadeh-Ghalehaziz, Shiva; Farahzadi, Raheleh; Fathi, Ezzatollah; Pashaiasl, Maryam

doi:10.15283/ijsc.2015.8.2.115

Cited by 33 publications

(17 citation statements)

References 38 publications

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“…These cells can be obtained by amniocentesis in the second trimester or end of pregnancy (Babaei, Gholizadeh Ghaleh Aziz, Alipour, & Naderi, ; Kang, Hwang, Kim, et al, ). It distinctive characteristics include having high self‐renewal and expression of OCT4, SOX2, PEX1, NANOG, LEFTY2, CRLPTO, and FGF‐4 which fortunately are also self‐renewal and pluripotency markers Kang, Yi, et al (); differentiating capacity of all cells into the three embryonic germ layers; positive for mesenchymal markers such as CD105 (SH2), CD73 (SH3/4), CD90, and CD166, but negative for hematopoietic markers CD31 and CD45 (Gholizadeh‐Ghalehaziz, Farahzadi, Fathi, & Pashaiasl, ). Furthermore, hAFMSCs have low immunogenicity, anti‐inflammation and nontumorigenic characteristics.…”

Section: Introductionmentioning

confidence: 99%

The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

Aziz

Fardyazar

Pashaiasl

2019

Molec Gen & Gen Med

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Purpose One of the most common malignancies peculiar to female health with few symptoms, low response to therapy, difficult diagnosis, frequent relapse, and high mortality, is ovarian cancer. Thus, our experiment, using Human amniotic fluid mesenchymal stem cells (hAFMSCs) as a therapeutic tool, aims to find an efficient treatment approach for patients suffering from SKOV3 ovarian cancer. Material & Methods In this study, we obtained 5 ml amniotic fluid from 16–20 week pregnant women who underwent amniocentesis for routine prenatal diagnosis by karyotyping in Al‐Zahra Hospital of Tabriz University of Medical Sciences, Iran. Using trans wells in 24 wells plate, hAFMSCs were isolated from all samples, co‐cultured with SKOV3 ovarian cancer cell line, and characterized via flow cytometry and RT‐PCR. Human skin fibroblast cells (HSFCs) were isolated and used as a negative control. SKOV3 and HSFCs' viability after 5 days was evaluated by MTT assay. Cell cycle and apoptotic genes were analyzed by real‐time PCR. Results We successfully isolated and characterized hAFMSCs through it positivity for CD44 and CD90 specific mesenchymal stem cell markers and negativity for CD31 and CD45. Oct4 and NANOG were evaluated by RT‐PCR as pluripotency markers, and visualized on 2% gel electrophoresis. We established hAFMS cell lines after 5 days of co‐culturing the SKOV3 cells, viability was decreased; however, HSFCs did not show toxicity by MTT assay. The genes indicated upregulation and high expression by a real‐time PCR. Conclusions Our findings showed that hAFMSCs have natural tumor tropism, and can release soluble factors in a cell culture, which cause an efficient anticancer effect. Thus, we can use hAFMSCs for complete anticancer therapy on SKOV3 cell line at cell culture condition and possibly in vivo in the near future.

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Section: Introductionmentioning

confidence: 99%

The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

Aziz

Fardyazar

Pashaiasl

2019

Molec Gen & Gen Med

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“…Certainly, stem cells could be the best choice. A slight influence on the stem cells may cause unexpected consequences to its related adult cells [30]. MSCs are pluripotent cells and are characterized by their self-renewal abilities through mitotic cell division, as well as by their potential to differentiate into multiple types of lineages such as: chondrogenic, osteogenic, adipogenic, myogenic, neurogenic, and hepatogenic [31, 32].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

Fathi

Farahzadi

2017

PLoS ONE

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Zinc ion as an essential trace element and electromagnetic fields (EMFs) has been reported to be involved in the regulation of bone metabolism. The aim of this study was to elucidate the effects of zinc sulphate (ZnSO4) on the osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ADSCs) in the presence of EMF as a strategy in osteoporosis therapy. Alkaline phophatase (ALP) activity measurement, calcium assay and expression of several osteoblastic marker genes were examined to assess the effect of ZnSO4 on the osteogenic differentiation of ADSCs under EMF. The expression of cAMP and PKA was evaluated by ELISA. The expression of β-catenin, Wnt1, Wnt3a, low-density lipoprotein receptor-related protein 5 (LRP5) and reduced dickkopf1 (DKK1) genes were used to detect the Wnt/β-catenin pathway. It was found that ZnSO4, in the presence of EMF, resulted in an increase in the expression of osteogenic genes, ALP activity and calcium levels. EMF, in the presence of ZnSO4, increased the cAMP level and protein kinase A (PKA) activity. Treatment of ADSCs with (MAPK)/ERK kinase 1/2 inhibitor, or PKA inhibitor, significantly inhibited the promotion of osteogenic markers, indicating that the induction of osteogenesis was dependent on the ERK and PKA signaling pathways. Real-time PCR analysis showed that ZnSO4, in the presence of EMF, increased the mRNA expressions of β-catenin, Wnt1, Wnt3a, LRP5 and DKK1. In this study, it was shown that 0.432 μg/ml ZnSO4, in the presence of 50 Hz, 20 mT EMF, induced the osteogenic differentiation of ADSCs via PKA, ERK1/2 and Wnt/β-catenin signaling pathways.

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“…In general, stem cells are classified based on their source of origin as embryonic stem cells and tissue specific / adult stem cells. Recently, Shinya Yamanaka reported of creating pluripotent stem cells from terminally differentiated fibroblasts [2][3][4][5][6][7][8][9][10][11][12]. Stem cells are also classified based on their differentiation potential -totipotent (early progeny of the zygote up to the eight cell stage of the morula), pluripotent (Inner cell mass of embryo, epiblast), multi/oligopotent (Fetal tissues, membranes, placenta and adult stem cells), bipotent (lymphoid or myeloid stem cells) or unipotent (Muscle stem cells) [13,14].…”

Section: Stem Cellsmentioning

confidence: 99%

Stem Cells and Metallothionein - A Review

2017

IJCRR

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Stem Cells are capable of self renewal and can differentiate into other lineages upon induction with specific growth factors and proteins. Mesenchymal stem cells obtained from various sources like adipose tissue, umbilical cord, bone marrow, umbilical cord blood have been reported to have stem cell characteristic features laid down by International Society for Cellular Therapy. Research so far deals with the differentiation and self renewal properties of stem cells, which needs to be understood further. Metallothionein, is a metal binding protein isolated from equine renal cortex and shown to be present in nearly all of our cells, with differential expression of it's isoforms. Apart from metal binding properties, this protein expression is deregulated in a variety of diseases and other treatment conditions. With respect to cancer biology, this protein expression is studied in almost all cancer types and results indicate pivotal role of this protein in cancer. Certain cancers are associated with up-regulation while some types are associated with down-regulation. The localization of this protein has also reported to change between actively proliferating and normal cells. Given the importance of this protein in cancer biology, the role of this protein in stem cell renewal as well as differentiation is poorly understood. This review discusses about stem cells, -its type and differentiation; metallothioenein -expression, function, review about different cancer types, role in angiogenesis and plausible role of this protein in the self renewal and differentiation of stem cells.

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A Mini Overview of Isolation, Characterization and Application of Amniotic Fluid Stem Cells

Cited by 33 publications

References 38 publications

The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

Stem Cells and Metallothionein - A Review

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