CD10+ Cells and IgM in Pathogen Response in Lumpfish (Cyclopterus lumpus) Eye Tissues

Abstract

Lumpfish (Cyclopterus lumpus), a North Atlantic “cleaner“ fish, is utilized to biocontrol salmon louse (Lepeophtheirus salmonis) in Atlantic salmon (Salmo salar) farms. Lumpfish require excellent vision to scan for and eat louse on salmon skin. The lumpfish eye immune response to infectious diseases has not been explored. We examined the ocular response to a natural parasite infection in wild lumpfish and to an experimental bacterial infection in cultured lumpfish. Cysts associated with natural myxozoan infection in the ocular scleral cartilage of wild adult lumpfish harbored cells expressing cluster of differentiation 10 (CD10) and immunoglobulin M (IgM). Experimental Vibrio anguillarum infection, which led to exophthalmos and disorganization of the retinal tissues was associated with disruption of normal CD10 expression, CD10+ cellular infiltration and IgM expression. We further describe the lumpfish CD10 orthologue and characterize the lumpfish scleral skeleton in the context of myxozoan scleral cysts. We propose that lumpfish develop an intraocular response to pathogens, exemplified herein by myxozoan and V. anguillarum infection involving novel CD10+ cells and IgM+ cells to contain and mitigate damage to eye structures. This work is the first demonstration of CD10 and IgM expressing cells in a novel ocular immune system component in response to disease in a teleost.

Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish

“…6C,D). The anti-lumpfish-IgM IgY antibody we previously used on lumpfish tissues stained spotted wolffish head kidney in a pattern consistent with that observed in lumpfish head kidney 6 (Supplemental Fig. 6G,H) and strongly stained leukocytic cells in the choroid body/rete mirabile with a pattern similar to Sox9 expression in this tissue (Supplemental Figs.…”

“…Our work nonetheless provides a basis to further explore the visual system of spotted wolffish, which are designated a threatened species in Canada. In previous work, we characterized the visual system of the North Atlantic lumpfish [1][2][3][4][5][6][7] . The work herein on spotted wolffish suggests some differences (scleral cartilage thickness, shape and reactivity) and similarities (expression of cellular and immune markers PCNA, IgM and CD45), between the two species.…”

“…We have recently characterized the visual system of the North Atlantic lumpfish (Cyclopterus lumpus) 1 . The characteristics we have observed in the eyes of this species and their importance as models of ocular and immune disorders [2][3][4][5][6][7] has prompted comparative analyses toward exploring the visual systems of other locally available North Atlantic marine teleosts as described in the work herein on spotted wolffish.…”

“…reported under stressful conditions 14,20 . Our previous work has described the morphological and molecular changes in ocular tissues of lumpfish experimentally challenged intraperitoneally with the marine pathogen V. anguillarum 4,6 but studies of the response of spotted wolffish eyes to exogenous insults have not been reported.…”

Immune response of silver pomfret (Pampus argenteus) to Amyloodinium ocellatum infection

“…IL1R2 participates in the negative regulation of IL-1 via competitive inhibition (Cardoso et al, 2020;Zhou et al, 2019). Studies have confirmed that CD40-related pathways are important for resistance against a variety of parasites (Gendron et al, 2020;Subauste, 2009).…”