Immune response of silver pomfret (<i>Pampus argenteus</i>) to <i>Amyloodinium ocellatum</i> infection

Zhang, Youyi; Hu, Jiabao; Li, Yaya; Zhang, Man; Jacques, Kimran Jean; Gu, Weiwei; Sun, Yibo; Sun, Jiachu; Yang, Yang; Xu, Shanliang; Wang, Yajun; Yan, Xiaojun

doi:10.1111/jfd.13524

Cited by 27 publications

(4 citation statements)

References 62 publications

(58 reference statements)

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“…Previous studies revealed that A. ocellatum infection induces a potent innate immune response in the host, including increased expression of genes linked to apoptosis and pro‐inflammation (Byadgi et al., 2019, 2021; Li et al., 2022; Zhang et al., 2021). In this view, Casp 3 , IL‐1 , IL‐10 and TNF‐α were selected to assess whether an in vitro platform could be used as a surrogate to examine parasite–host interactions.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Li,

Zhuang,

Chen

et al. 2024

Journal of Fish Diseases

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Amyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti‐parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular levels. The present work developed a new cell line, ALG, from the gill of yellowfin seabream (Acanthopagrus latus). The cell line was routinely cultured at 28°C in Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum (FBS). ALG cells were adherent and exhibited an epithelioid morphology; the cells were stably passed over 30 generations and successfully cryopreserved. The cell line derived from A. latus was identified based on partial sequence amplification and sequencing of cytochrome B (Cyt b). The ALG was seeded onto transwell inserts and found to be a platform for in vitro infection of A. ocellatum, with a 37.23 ± 5.75% infection rate. Furthermore, scanning electron microscopy (SEM) revealed that A. ocellatum parasitizes cell monolayers via rhizoids. A. ocellatum infection increased the expression of apoptosis and inflammation‐related genes, including caspase 3 (Casp 3), interleukin 1 (IL‐1), interleukin 10 (IL‐10), tumour necrosis factor‐alpha (TNF‐α), in vivo or in vitro. These results demonstrated that the in vitro gill cell monolayer successfully recapitulated in vivo A. latus host responses to A. ocellatum infection. The ALG cell line holds great promise as a valuable tool for investigating parasite–host interactions in vitro.

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Section: Discussionmentioning

confidence: 99%

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Li,

Zhuang,

Chen

et al. 2024

Journal of Fish Diseases

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“…Fish were manually fed two times a day at 08:00 a.m. and 17:00 p.m. with commercial feed (larvae love 6#, Japan), until satiety. The experimental condition was controlled according to a previous study [41]. Throughout the experiment, the water temperature ranged between 25.5 • C and 27.5 • C, salinity was 24 ± 0.89%, and dissolved oxygen was 7.3 ± 0.49 mg/L.…”

Section: Fishmentioning

confidence: 99%

Molecular Characterization and Expression Response of Ghrelin, GLP-1 and PYY to Fasting, Dietary Lipid, and Fatty Acids in Silver Pomfret (Pampus argenteus)

Zhang

Shi

Zhang

et al. 2023

Fishes

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Ghrelin, glucagon-like peptide-1 (GLP-1), and peptide YY (PYY) are potent hormones mediating food intake according to the nutritional status in fish. However, limited information is available on these genes and their expression in response to nutrition in silver pomfret (Pampus argenteus). A comparison analysis revealed that ghrelin and GLP-1 were relatively conserved in marine fish. PYYa and PYYb shared a low identity and were clustered to different PYY branches. Ghrelin, pyya, and pyyb mRNAs were highly expressed in the brain, while glp-1 was highly expressed in the gills and liver. The ghrelin mRNA expression was relatively high an hour post-feeding and decreased after 3 to 72 h of fasting. The glp-1 mRNA expression was increased after 6 to 24 h of fasting. The pyya mRNA expression was increased after 72 h of fasting, while the pyyb expression remained stable during fasting. The diet with 14% lipid promoted the expressions of glp-1, pyya, and pyyb, but inhibited the ghrelin expression. The ghrelin expression in the intestine was increased after palmitic acid, oleic acid (OA), linoleic acid (LA), α-linolenic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) incubation. OA decreased the expression of glp-1 and increased the expression of pyya. The expression of pyyb was downregulated by LA, DHA, and EPA. These results suggest the importance of ghrelin, glp-1, and pyy in coordinating food intake in response to fasting, dietary lipid concentration, and fatty acids in silver pomfret.

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“…In order to develop more effective prophylactic/therapeutic controls, a better understanding of the mechanisms underlying host-parasite interactions is a key requirement. Detailed descriptions of changes provoked by AO in the gill epithelium of different host species have been provided by previous authors, who furnished consistent reports of these lesions alluding also to the mechanisms involved in the host response [4,5,[7][8][9][10][11]. Ultrastructural studies have better clarified the morphology of the parasite and the stomopode conformation/composition, including aspects of parasite-host interaction with three cyprinodontid species [12].…”

Section: Introductionmentioning

confidence: 98%

News Insights into the Host-Parasite Interactions of Amyloodiniosis in European Sea Bass: A Multi-Modal Approach

et al. 2022

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Amyloodiniosis is a disease resulting from infestation by the ectoparasitic dinoflagellate Amyloodinium ocellatum (AO) and is a threat for fish species such as European sea bass (ESB, Dicentrarchus labrax), which are farmed in lagoon and land-based rearing sites. During the summer, when temperatures are highest, mortality rates can reach 100%, with serious impacts for the aquaculture industry. As no effective licensed therapies currently exist, this study was undertaken to improve knowledge of the biology of AO and of the host-parasite relationship between the protozoan and ESB, in order to formulate better prophylactic/therapeutic treatments targeting AO. To achieve this, a multi-modal study was performed involving a broad range of analytical modalities, including conventional histology (HIS), immunohistochemistry (IHC) and confocal laser scanning microscopy (CLSM). Gills and the oro-pharyngeal cavity were the primary sites of amyloodiniosis, with hyperplasia and cell degeneration more evident in severe infestations (HIS). Plasmacells and macrophages were localised by IHC and correlated with the parasite burden in a time-course experimental challenge. CLSM allowed reconstruction of the 3D morphology of infecting trophonts and suggested a protein composition for its anchoring and feeding structures. These findings provide a potential starting point for the development of new prophylactic/therapeutic controls.

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Immune response of silver pomfret (Pampus argenteus) to Amyloodinium ocellatum infection

Cited by 27 publications

References 62 publications

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Molecular Characterization and Expression Response of Ghrelin, GLP-1 and PYY to Fasting, Dietary Lipid, and Fatty Acids in Silver Pomfret (Pampus argenteus)

News Insights into the Host-Parasite Interactions of Amyloodiniosis in European Sea Bass: A Multi-Modal Approach

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