Cd, Cu, Ni, Mn and Zn resistance and bioaccumulation by thermophilic bacteria, Geobacillus toebii subsp. decanicus and Geobacillus thermoleovorans subsp. stromboliensis

Özdemir, Sadin; Kılınç, Ersin; Poli, Annarita; Nicolaus, Barbara; Güven, Kemal

doi:10.1007/s11274-011-0804-5

Cited by 47 publications

(12 citation statements)

References 38 publications

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“…1000 µg mL −1 of Co(II) and Hg(II) solutions were buyed from High Purity Standards, Charleston, SC, USA). Concentrated H 2 O 2 (35%), HNO 3 (65%), HCl (36.5-38.0%) and NH 4 OH were obtained from Sigma Aldrich, Germany. NCSZC 73014 tea leaves, NCS DC78301 river sediment and DORM2dogfish muscle were already available in the laboratory.…”

Section: Methodsmentioning

confidence: 99%

“…The pellet was digested in HNO 3 before determination. The quantity of biosorbed Co(II) and Hg(II) was calculated using the equation given in literature 38 . The highest capacity of the thermophilic A. kestanboliensis loaded onto XAD-4 column was found to be 24.3 and 27.8 mg g −1 for Co(II) and Hg(II), respectively.…”

Section: Analytical Figures Of Merit Analytical Figures Of Merit Wermentioning

confidence: 99%

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A Novel Biosorbent for Preconcentrations of Co(II) and Hg(II) in Real Samples

Özdemir

Kılınç

Şen

2020

Sci Rep

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A new biosorbent, composed of Amberlite XAD-4 loaded with Anoxybacillus kestanboliensis, was developed and surface morphologies were investigated by SeM and ft-iR. it was used for solid phase column preconcentrations of co(ii) and Hg(ii) before their measurements by icp-oeS. LoDs were calculated as 0.04 and 0.06 ng mL −1 for co(ii) and Hg(ii) respectively. the maximum biosorption capacities were determined as 24.3 and 27.8 mg g −1 for co(ii) and Hg(ii) respectively. preconcentration factors were achieved for Co(II) and Hg(II) as 80. The method validation was performed by analyzing certified reference materials. The new process was successfully utilized for the preconcentration of these metals in various food samples. it should be highlighted that the sensitivity of icp-oeS was critically improved by applying developed method. Hence, ICP-OES could be an effective alternative for icp-MS and/or Gf-AAS.Some metal ions are known to be highly toxic. Therefore, a number of recent studies have focused on metal ion toxicity and carcinogenicity on human beings and other living organisms even at very low levels 1,2 . Among the various types of pollutants, toxic metal ions cannot be easily eliminated from nature 3 . Accumulation of toxic and deleterious metal ions in soft tissues of animals and humans even at low concentrations could be a major concern as they are not metabolized and can create significant damage to the body tissues 4 . Co and Hg are two typical metal ions in environmental samples which are also detected in plants and food samples. Toxicological influences such as vasodilation and cardiomyopathy are reported for Co exposure. Hg is also familiar as it is the most neurotoxic metal ion and can harm most of the human systems 3 .The usage of the biological processes is an idea for the operation of environmental metal pollution. Biological processes for the bioremediation depend on the physical nature of binding sites and chemical conditions 4 . The utilization of biomass, including alive and dead microorganisms in the possibly retention and removal of metal ions from environmental samples, has acquired significant reliability over the past few years due to its efficiency, ecofriendliness and economic choice. The major properties of biosorbents are (a) having a variety of metal binding sites, (b) having small and identical sizes, (c) being less subject to interference from alkali-earth and alkali metals than ion-exchange resins (d) being generally cheaper than functionalized polymers (e) having the possibility to be obtained via green technologies 5 .Concentrations of toxic metal ions are known as trace levels that are lower than the quantification limit of the instruments 6 . The determination, separation and analysis of low concentration of toxic metal ions in natural samples such as soil, food and water have become significant 7 . Therefore, it is necessary to develop analytical methods to preconcentrate and determine trace metal ions 6 .Alternative methods are recommended for the preconcentration and separatio...

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Section: Methodsmentioning

confidence: 99%

Section: Analytical Figures Of Merit Analytical Figures Of Merit Wermentioning

confidence: 99%

A Novel Biosorbent for Preconcentrations of Co(II) and Hg(II) in Real Samples

Özdemir

Kılınç

Şen

2020

Sci Rep

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“…Thermophilic endospore-formers constitute an excellent resource of biotechnologically important products and can be used as the producers of these proteins [6][7][8][9][10]. It is essential to monitor the purity of the protein-producing strains in these processes.…”

Section: Introductionmentioning

confidence: 99%

Characterization and evaluation of tandem repeats for the identification of Geobacillus

Krilavičiūtė

Kuisienė

2013

Open Life Sciences

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Taxonomy of thermophilic, endospore-forming bacteria has evoked a great interest over the past few years. Although a number of taxonomic markers were previously evaluated, their sequences in Geobacillus were too conservative, and identification of more variable markers is needed. Repetitive DNA is one of the promising variable targets in the development of the taxon-specific genotyping and identification schemes in bacteria. The aim of our study was to evaluate the possibility of using repetitive DNA in the taxonomy of Geobacillus. In this paper, we report the analysis of perfect tandem repeats of geobacilli. We focused on the long repeats (with a motif length of ≥20 nucleotides). This choice was based on the assumption that these motifs can be used for the construction of oligonucleotides — primers and probes. Thirty-three Geobacillus genus-specific motifs were identified in our work, fifteen of them were species-specific and fifteen — species cluster -specific. Three of them were genus-, but not species- or species cluster-specific. Some of the motifs were used for the construction of the primer pairs. The primers were validated by PCR. Out of 12 designed primer pairs, 11 were genus-specific and 4 — species-specific. Species-specific primers were successfully constructed for the phylogenetically defined species Geobacillus thermodenitrificans and Geobacillus toebii.

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“…[4][5][6][7] Another strain can produce acetoin and 2,3-butanediol. 8) Some others have the ability to utilize cellulose 9) or hemicelluloses, 10) to accumulate arsenate 11) or toxic metal ions 12) and to degrade hydrocarbons, 13) long-chain alkanes, 14,15) herbicides, 2) or polyvinyl alcohol. 16) These properties make Geobacillus spp.…”

mentioning

confidence: 99%

A thiostrepton resistance gene and its mutants serve as selectable markers inGeobacillus kaustophilusHTA426

Wada

Kobayashi

Furukawa

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Effective utilization of microbes often requires complex genetic modification using multiple antibiotic resistance markers. Because a few markers have been used in Geobacillus spp., the present study was designed to identify a new marker for these thermophiles. We explored antibiotic resistance genes functional in Geobacillus kaustophilus HTA426 and identified a thiostrepton resistance gene (tsr) effective at 50 °C. The tsr gene was further used to generate the mutant tsr(H258Y) functional at 55 °C. Higher functional temperature of the mutant was attributable to the increase in thermostability of the gene product because recombinant protein produced from tsr(H258Y) was more thermostable than that from tsr. In fact, the tsr(H258Y) gene served as a selectable marker for plasmid transformation of G. kaustophilus. This new marker could facilitate complex genetic modification of G. kaustophilus and potentially other Geobacillus spp.

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Cd, Cu, Ni, Mn and Zn resistance and bioaccumulation by thermophilic bacteria, Geobacillus toebii subsp. decanicus and Geobacillus thermoleovorans subsp. stromboliensis

Cited by 47 publications

References 38 publications

A Novel Biosorbent for Preconcentrations of Co(II) and Hg(II) in Real Samples

A Novel Biosorbent for Preconcentrations of Co(II) and Hg(II) in Real Samples

Characterization and evaluation of tandem repeats for the identification of Geobacillus

A thiostrepton resistance gene and its mutants serve as selectable markers inGeobacillus kaustophilusHTA426

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