2013
DOI: 10.2478/s11535-013-0168-3
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Abstract: Taxonomy of thermophilic, endospore-forming bacteria has evoked a great interest over the past few years. Although a number of taxonomic markers were previously evaluated, their sequences in Geobacillus were too conservative, and identification of more variable markers is needed. Repetitive DNA is one of the promising variable targets in the development of the taxon-specific genotyping and identification schemes in bacteria. The aim of our study was to evaluate the possibility of using repetitive DNA in the ta… Show more

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“…In total, 33 Geobacillus genus-specific motifs with length over 20 nucleotides were determined: 3 were genus-specific, 15-species-specific, and 15-species cluster-specific. Motifs have been used for the construction of the genus-and species-specific (G. thermodenitrificans and G. toebii) primer pairs [44]. Rep-PCR molecular method based on the usage of outwardly facing oligonucleotide PCR primers complementary to interspersed repetitive sequences is widely applied in the characterization of different groups of bacteria.…”
Section: Geobacillus Bacteria: Taxonomy and Identificationmentioning
confidence: 99%
“…In total, 33 Geobacillus genus-specific motifs with length over 20 nucleotides were determined: 3 were genus-specific, 15-species-specific, and 15-species cluster-specific. Motifs have been used for the construction of the genus-and species-specific (G. thermodenitrificans and G. toebii) primer pairs [44]. Rep-PCR molecular method based on the usage of outwardly facing oligonucleotide PCR primers complementary to interspersed repetitive sequences is widely applied in the characterization of different groups of bacteria.…”
Section: Geobacillus Bacteria: Taxonomy and Identificationmentioning
confidence: 99%