Cd 2+ and Pb 2+ complexation by glutathione and the phytochelatins

Jacquart, Aurélie; Brayner, Roberta; Chahine, Jean‐Michel El Hage; Ha-Duong, Nguyêt-Thanh

doi:10.1016/j.cbi.2016.09.002

Cited by 50 publications

(35 citation statements)

References 38 publications

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“…The relatively soft acid character of Cd 2+ leads to a strong affinity for soft sulphur donor atoms, especially for thiolates, for instance in cysteine residues (Mah and Jalilehvand, 2010). The Cd-GSH and Cd-PC complexes have been determined and characterized in vitro (Cruz et al, 2005;Chekmeneva et al, 2007;Leverrier et al, 2007;Chekmeneva et al, 2008;Mah and Jalilehvand, 2010;Jacquart et al, 2017). In vivo, the existence, the distribution and the formation kinetics of Cd-PC complexes was supported by analyses using X-ray absorption spectroscopy (Salt et al, 1995;Salt et al, 1997), small-scale gel filtration (Rauser, 2003) or fractionation techniques coupled to mass spectrometry (Persson et al, 2006).…”

Section: A Glutathione and Phytochelatinsmentioning

confidence: 99%

Mechanisms of Cadmium Accumulation in Plants

Sterckeman

Thomine

2020

Critical Reviews in Plant Sciences

171

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Cadmium is a non-essential trace metal, which is highly toxic to nearly all living organisms. Soil pollution causes Cd contamination of crops, thereby rendering plant products responsible for the chronic low level Cd over-exposure of numerous populations in the world. For this reason, Cd accumulation in plants has been studied for about five decades now. The research first focused on the relationships between plant and soil Cd levels, on the factors of the metal availability in soil, as well as the root uptake processes. Cd distribution in plant organs was also investigated, first using a macroscopic and eco-physiological approach, then with the help of molecular biology tools, at both tissue and cell scale. Cadmium has no biological function and hijacks the transport pathways of micronutrients such as Fe, Mn or Zn, in order to enter the plant through the roots and be distributed to all its organs. The study of the genes that control the influx and efflux of the Cd 2 + ion in the cytosol, vacuoles and vascular tissues has significantly contributed to the understanding of the metal root uptake and of its transfer to the aerial parts. However, the mechanisms responsible for its distribution to the different aboveground tissues and specially to fruits and seeds have yet to be clarified. This review summarizes current knowledge in order to present a detailed overview of Cd transport and storage, from the rhizosphere to the different organs and tissues of the plant.

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Section: A Glutathione and Phytochelatinsmentioning

confidence: 99%

Mechanisms of Cadmium Accumulation in Plants

Sterckeman

Thomine

2020

Critical Reviews in Plant Sciences

171

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“…GSH has a crucial role to detoxify heavy metals. As the precursor of phytochelatins (PCs), GSH is also involved in the sequestration of heavy metals in vacuole [18,19]. Researchers reported that exogenous GSH alleviated Cd toxicity in plants by reducing or maintaining Cd uptake [20][21][22], while few studies have examined the subcellular distributions and chemical forms of Cd with GSH treatment [23].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Glutathione Alleviation of Cd Toxicity in Italian Ryegrass (Lolium multiflorum) by Modulation of the Cd Absorption, Subcellular Distribution, and Chemical Form

Fang

Yin

et al. 2020

IJERPH

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Subcellular fractions and the chemical forms of cadmium (Cd) reflect its level of toxicity to plants; however, these effects of exogenous glutathione (GSH) are poorly understood. We exposed two Italian ryegrass (Lolium multiflorum) cultivars (IdyII and Harukaze) to 50 µM Cd or 200 µM GSH to investigate the effect of GSH on the Cd uptake, subcellular compartments, and chemical forms. Cd significantly inhibited the plant growth, while GSH supplementation decreased this inhibition. The application of GSH significantly improved the Cd concentration in the roots but reduced that in the shoots and decreased the Cd translocation from root to shoot. The Cd concentration of the root in the cell wall was increased while the concentration in the soluble fraction was decreased when supplied with GSH. The inorganic form (80% ethanol for Cd extraction) in the roots was significantly reduced when treated with GSH. The Cd form extracted by 2% acetic acid (HAC) with low toxicity and immobility were greatly increased. In leaves, the application GSH decreased in any form of Cd form extracted. In conclusion, exogenous GSH decreased the translocation of Cd and alleviated Italian ryegrass Cd toxicity by accumulating more Cd in the root cell wall and immobilizing more Cd in lower toxicity fractions.

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“…Data from the GO analysis, regarding the GST enzyme gene in this work, indicate that GST mainly plays a role in the toxin metabolic process and the response to metal ions ( Figures S4 and S5). Alternately, PCs may play an important role in the detoxification of heavy metals in this process [60]. Data analyses indicate that the expression of the glutamate-cysteine ligase catalytic subunit (103861360), which participates in cysteine and methionine metabolism following GSH synthesis and synthesis of subsequent chelating agents (e.g., PCs) with GSH as a precursor, were up-regulated in plants under Cd stress (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress

Sun

Cao

et al. 2019

IJMS

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Brassica campestris L., a hyperaccumulator of cadmium (Cd), is considered a candidate plant for efficient phytoremediation. The hairy roots of Brassica campestris L are chosen here as a model plant system to investigate the response mechanism of Brassica campestris L. to Cd stress. High-throughput sequencing technology is used to identify genes related to Cd tolerance. A total of 2394 differentially expressed genes (DEGs) are identified by RNA-Seq analysis, among which 1564 genes are up-regulated, and 830 genes are down-regulated. Data from the gene ontology (GO) analysis indicate that DEGs are mainly involved in metabolic processes. Glutathione metabolism, in which glutathione synthetase and glutathione S-transferase are closely related to Cd stress, is identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A Western blot shows that glutathione synthetase and glutathione S-transferase are involved in Cd tolerance. These results provide a preliminary understanding of the Cd tolerance mechanism of Brassica campestris L. and are, hence, of particular importance to the future development of an efficient phytoremediation process based on hairy root cultures, genetic modification, and the subsequent regeneration of the whole plant.

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Cd 2+ and Pb 2+ complexation by glutathione and the phytochelatins

Cited by 50 publications

References 38 publications

Mechanisms of Cadmium Accumulation in Plants

Mechanisms of Cadmium Accumulation in Plants

Exogenous Glutathione Alleviation of Cd Toxicity in Italian Ryegrass (Lolium multiflorum) by Modulation of the Cd Absorption, Subcellular Distribution, and Chemical Form

Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress

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