CC Chemokine and CC Chemokine Receptor Profiles in Visceral and Subcutaneous Adipose Tissue Are Altered in Human Obesity

Huber, J.F.K.; Kiefer, Florian W.; Zeyda, Maximilian; Ludvik, Bernhard; Silberhumer, Gerd R.; Prager, Gerhard; Zlabinger, Gerhard J.; Stulnig, Thomas M.

doi:10.1210/jc.2007-2630

Cited by 289 publications

(269 citation statements)

References 39 publications

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“…Upregulation by obesity was similar in intra-abdominal visceral and subcutaneous adipose tissue. Similarly, expression of the macrophage marker CD68 (Figure 1b) and macrophage count 25 was upregulated by obesity in both visceral and subcutaneous adipose tissue to a comparable extent. CD163 expression strictly correlated with CD68 (r ¼ 0.952 and 0.920 for obese visceral and subcutaneous adipose tissue, respectively; Po0.001 for both).…”

Section: Resultsmentioning

confidence: 72%

“…The trend towards lower visceral adipose expression of heme oxygenase-1 in obesity was also apparent on the protein level ( Figure 2b). As the expression of both HMOX1 (Figure 2d) and CD68 26 is confined to macrophages in adipose tissue and CD68 accurately reflected the number of macrophages in adipose tissue sections of the study population (Huber et al 25 and Adipose heme oxygenase-1 and insulin sensitivity S Shakeri-Manesch et al data not shown), the ratio of HMOX1 and CD68 was calculated as a measure of HMOX1 expression in adipose tissue macrophages. HMOX1/CD68 was significantly elevated by obesity in subcutaneous and visceral adipose tissue (Figure 2d).…”

Section: Regulation Of Heme Oxygenase-1 Expression In Obesitymentioning

confidence: 94%

“…24 Characteristics of the study population including serum C-reactive protein have been published elsewhere. 25 Analysis of gene expression Tissue specimens were washed in saline buffer, blood and visible blood vessels were removed and tissues immediately snap-frozen in liquid nitrogen. Total RNA was prepared by disrupting adipose tissue in TRIzol reagent (Invitrogen, Carlsbad, CA, USA) with a tissue homogenizer followed by RNA isolation according the manufacturer's instructions.…”

Section: Metabolic Parametermentioning

confidence: 99%

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Diminished upregulation of visceral adipose heme oxygenase-1 correlates with waist-to-hip ratio and insulin resistance

et al. 2009

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Objective: Insulin resistance in visceral obesity is substantially driven by adipose tissue inflammation, particularly by macrophages accumulating in obese adipose tissue. In contrast, adipose tissue macrophages express the hemoglobin scavenger receptor (CD163) and heme oxygenase-1 (gene: HMOX1) that together protect from oxidative stress. Our aim was to evaluate the expression of CD163 and HMOX1 in intra-abdominal visceral (omental) and subcutaneous adipose tissue as well as circulating soluble CD163 concentrations in human obesity and its association with adipose tissue inflammation, body fat distribution and insulin resistance. Methods: CD163, HMOX1 and CD68 mRNA expression in visceral and subcutaneous adipose tissue, serum concentration of soluble CD163 in morbidly obese patients (body mass index (BMI) 440 kg m À2 ), who underwent laparoscopic surgery for gastric banding (n ¼ 20), matched for age and sex to controls (BMIo30 kg m À2 ; n ¼ 20) was analyzed. Results: CD163 expression was highly upregulated in human adipose tissue and soluble CD163 serum concentration was elevated in obese vs lean subjects. HMOX1 was upregulated in adipose tissue by obesity as well and expressed predominantly in macrophages. Although CD163 expression strictly correlated with macrophage abundance, HMOX1 was additionally upregulated within macrophages. This upregulation was significantly lower in visceral compared with subcutaneous adipose tissue. Strikingly, relative visceral adipose tissue expression of HMOX1 negatively correlated with waist-to-hip ratio and the homeostasis model assessment of insulin resistance (both P ¼ 0.024). Conclusions: Visceral obesity is associated with defective upregulation of heme oxygenase-1 in visceral adipose tissue. A lack of this antioxidative and anti-inflammatory enzyme in visceral adipose tissue could contribute to the development of insulin resistance.

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Section: Resultsmentioning

confidence: 72%

Section: Regulation Of Heme Oxygenase-1 Expression In Obesitymentioning

confidence: 94%

Section: Metabolic Parametermentioning

confidence: 99%

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Diminished upregulation of visceral adipose heme oxygenase-1 correlates with waist-to-hip ratio and insulin resistance

et al. 2009

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“…Adipose tissue is thought to be a source of chemokines. Notably, Huber et al reported that CC chemokines such as CCL5 (regulated on activation, normal T cell expressed and secreted; RANTES) are high in obese subjects and that their respective receptors are up-regulated in visceral adipose tissue (VAT) [8]. Recent studies revealed that obesity induces significant changes in the circulating levels of adipocytokines such as adiponectin and tumor necrosis factor in children and adolescents [9][10][11].…”

mentioning

confidence: 99%

CCL2 level is elevated with metabolic syndrome and CXCL10 level is correlated with visceral fat area in obese children

et al. 2016

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“…In humans, recent studies have also shown up regulation of CCR5 in the visceral fat of morbidly obese individuals in whom macrophage infiltration has been confirmed [20].…”

Section: Obesity and White Blood Cells (Wbcs):-mentioning

confidence: 96%

Relationship Between Obesity and Immune System a Review Article

el-Fetoh¹,

Aodh²,

Alanazi³

et al. 2016

IJAR

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CC Chemokine and CC Chemokine Receptor Profiles in Visceral and Subcutaneous Adipose Tissue Are Altered in Human Obesity

Abstract: Up-regulation of the CC chemokines and their respective receptors in adipose tissue occurs in human obesity and is associated with increased systemic inflammation.

Cited by 289 publications

References 39 publications

Diminished upregulation of visceral adipose heme oxygenase-1 correlates with waist-to-hip ratio and insulin resistance

Diminished upregulation of visceral adipose heme oxygenase-1 correlates with waist-to-hip ratio and insulin resistance

CCL2 level is elevated with metabolic syndrome and CXCL10 level is correlated with visceral fat area in obese children

Relationship Between Obesity and Immune System a Review Article

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