CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis

Shin, Jong Son; So, Won Mi; Kim, Soo Youn; Noh, Minsoo; Hyoung, Sujin; Yoo, Kyoung Shin; Shin, Jeong Sheop

doi:10.1016/j.plantsci.2020.110458

Cited by 27 publications

(22 citation statements)

References 46 publications

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“…The overexpression of soybean CDCP genes, namely GmCBS21 and GmCBSDUF3, have been reported to impart tolerance to low nitrogen and multiple abiotic stresses, respectively [14,15]. In Arabidopsis, AtCBSX1 and AtCBSX3 have been identified to interact with the thioredoxins (Trxs) in the chloroplast and mitochondria, respectively, and thereby regulate the ROS levels in the respective organelles [16,17]. The chloride channel sub-family of CDCPs in plants has been inferred to function in nitrate transport and Cl − sequestration [18].…”

Section: Introductionmentioning

confidence: 99%

Genetic Conservation of CBS Domain Containing Protein Family in Oryza Species and Their Association with Abiotic Stress Responses

Tomar

Subba

Bala

et al. 2022

IJMS

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Crop Wild Relatives (CWRs) form a comprehensive gene pool that can answer the queries related to plant domestication, speciation, and ecological adaptation. The genus ‘Oryza’ comprises about 27 species, of which two are cultivated, while the remaining are wild. Here, we have attempted to understand the conservation and diversification of the genes encoding Cystathionine β-synthase (CBS) domain-containing proteins (CDCPs) in domesticated and CWRs of rice. Few members of CDCPs were previously identified to be stress-responsive and associated with multiple stress tolerance in rice. Through genome-wide analysis of eleven rice genomes, we identified a total of 36 genes encoding CDCPs in O. longistaminata, 38 in O. glaberrima, 39 each in O. rufipogon, O. glumaepatula, O. brachyantha, O. punctata, and O. sativa subsp. japonica, 40 each in O. barthii and O. meridionalis, 41 in O. nivara, and 42 in O. sativa subsp. indica. Gene duplication analysis as well as non-synonymous and synonymous substitutions in the duplicated gene pairs indicated that this family is shaped majorly by the negative or purifying selection pressure through the long-term evolution process. We identified the presence of two additional hetero-domains, namely TerCH and CoatomerE (specifically in O. sativa subsp. indica), which were not reported previously in plant CDCPs. The in silico expression analysis revealed some of the members to be responsive to various abiotic stresses. Furthermore, the qRT-PCR based analysis identified some members to be highly inducive specifically in salt-tolerant genotype in response to salinity. The cis-regulatory element analysis predicted the presence of numerous stress as well as a few phytohormone-responsive elements in their promoter region. The data presented in this study would be helpful in the characterization of these CDCPs from rice, particularly in relation to abiotic stress tolerance.

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Section: Introductionmentioning

confidence: 99%

Genetic Conservation of CBS Domain Containing Protein Family in Oryza Species and Their Association with Abiotic Stress Responses

Tomar

Subba

Bala

et al. 2022

IJMS

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“…OsDGK8 plays a role in generating phosphatidic acid (PA) in response to biotic and abiotic stresses (Li et al, 2015), suggesting that it may participate in PA-dependent ROS production. OsCBSX10, UDP-glycosyltransferase, Yr10-like protein, OsRING-1 and their Arabidopsis homologs were documented to perform functions in mitochondrial ROS accumulation, pathogen-induced oxidative burst as well as hypersensitive responses (Meng et al, 2006;Coram et al, 2010;Shin et al, 2020). Thus, these data collectively suggest that downregulation of such genes in idr1-1 mutant presumably attenuates ROS production and cell death in different cellular compartments or tissues.…”

Section: Transcriptome Analyses Of Drought-stressed Idr1-1 Mutants Rementioning

confidence: 81%

Mutation ofIDR1enhances drought tolerance by reducing ROS production and activating ROS scavenging in rice

Wang

et al. 2020

Preprint

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To discover new mutant alleles conferring enhanced tolerance to drought stress, we screened a mutagenized rice population (cv. IAPAR9) and identified a mutant, named idr1-1 (for increased drought resistance 1-1), with obviously increased drought tolerance under upland field conditions. The idr1-1 mutant possessed a significantly enhanced ability to tolerate high-drought stress in different trials. Map-based cloning revealed that the gene LOC_Os05g26890 (corresponding to D1 or RGA1 gene), residing in the mapping region of IDR1 locus, carried a single-base deletion in the idr1-1 mutant, which caused a frameshift and premature translation termination. Complementation tests indicated that such a mutation was indeed responsible for the elevated drought tolerance in idr1-1 mutant. IDR1 protein was localized in nucleus and to plasma membrane or cell periphery. Further investigations indicated that the significantly increased drought tolerance in idr1-1 mutant stemmed from a range of physiological and morphological changes occurring in such a mutant, including greater leaf potentials, increased proline contents, heightened leaf thickness, and upregulation of antioxidant-synthesizing and drought-induced genes, etc., under drought-stressed conditions. Especially, ROS production from NADPH oxidases and chloroplasts might be remarkably impaired, while ROS-scavenging ability appeared to be markedly enhanced as a result of significantly elevated expression of a dozen ROS-scavenging enzyme genes in idr1-1 mutant under drought-stressed conditions. Besides, IDR1 physically interacted with TUD1, and idr1-1 mutant showed impaired EBR responsiveness. Altogether, these results suggest that mutation of IDR1 leads to alterations of multiple layers of regulations, which ultimately confers obviously enhanced drought tolerance to the idr1-1 mutant.One-sentence summaryMutation of IDR1 significantly enhances drought tolerance in an upland cultivar IAPAR9 by decreasing apoplastic and chloroplastic ROS production and increasing ROS-scavenging ability

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“…The CBS domain-containing proteins are a large superfamily of proteins that conserve CBS domains, which play a regulatory role for many enzymes. It has been reported that CBSX1 and CBSX2 are related to the level of ROS activated by thioredoxin and directly involved in the deposition of lignin (Wook et al, 2013 ; Shin et al, 2020 ). Moreover, there is research evidence to confirm that CBSX3 knockdown plants are severely sterile, which is caused by the failure of anther dehiscence due to secondary wall thickening (Shin et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that CBSX1 and CBSX2 are related to the level of ROS activated by thioredoxin and directly involved in the deposition of lignin (Wook et al, 2013 ; Shin et al, 2020 ). Moreover, there is research evidence to confirm that CBSX3 knockdown plants are severely sterile, which is caused by the failure of anther dehiscence due to secondary wall thickening (Shin et al, 2020 ). In sterile wheat anthers caused by HT, the anther wall does not display dehiscence, and the expression of CBS domain-containing protein CBSX6-like (CBSX6-L) gene ( TraesCS7D01G259800 ) is downregulated in sterile anthers ( Figure 9 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Male Sterile Anthers Induced by High Temperature in Wheat (Triticum aestivum L.)

Liu

Sun

et al. 2021

Front. Plant Sci.

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Global warming will have a negative effect on agricultural production as high temperature (HT) stress can seriously threaten plant growth and reproduction. Male sterility caused by HT may be exploited by the creation of a male-sterile line, which has great potential for application in crop heterosis. Therefore, it is important to understand the molecular mechanisms of anther abortion induced by HT in wheat, which remain unclear at present. In this study, we performed phenotype improve language in the abstract and comparative transcriptome analysis of the male sterile anthers induced by HT in wheat. Compared with Normal anthers, the cytological analysis indicated that HT-induced male sterile anthers were smaller and had no starch accumulation in pollen grains, which is consistent with the results observed by scanning electron microscopy (SEM). The 9601 differentially expressed genes (DEGs) identified by transcriptome sequencing compared with the Normal anthers were noticeably involved in the following pathways: starch and sucrose metabolism, phosphatidylinositol (PI) signaling system, peroxidase activity and response to oxidative stress, and heme binding. In addition, TUNEL assays were performed and the results further confirmed the excessive accumulation of reactive oxygen species (ROS) in sterile anthers. Moreover, a total of 38 hub genes were obtained from the protein-protein interaction network analysis of these pathways, including genes, for example, heat shock protein 90 (HSP90), thioredoxin-like protein 1, peroxidase (POD), calreticulin, UDP glucose pyrophosphorylase (UGPase), sucrose synthase, phosphatidylinositol-4-phosphate 5-Kinase (PIP5K), cytochrome c, and Cystathionine beta-synthase X6-like (CBSX6-like). These findings provide insights for predicting the functions of the candidate genes, and the comprehensive analysis of our results is helpful for studying the abortive interaction mechanism induced by HT in wheat.

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CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis

Cited by 27 publications

References 46 publications

Genetic Conservation of CBS Domain Containing Protein Family in Oryza Species and Their Association with Abiotic Stress Responses

Genetic Conservation of CBS Domain Containing Protein Family in Oryza Species and Their Association with Abiotic Stress Responses

Mutation ofIDR1enhances drought tolerance by reducing ROS production and activating ROS scavenging in rice

Comparative Transcriptome Analysis of Male Sterile Anthers Induced by High Temperature in Wheat (Triticum aestivum L.)

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