Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization

Song, Zhuo; Li, Bo; Li, Mengyuan; Luo, Jiamei; Hong, Yuqi; He, Yu‐Ying; Chen, Siting; Yang, Zeng-Ming; Chen, Liang; Yang, Zeng‐Ming

doi:10.3390/ijms23073699

Cited by 11 publications

(5 citation statements)

References 58 publications

(76 reference statements)

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“…Mouse uterine epithelial cells were isolated as previously described ( 84 ). Mouse uterine stromal cells were isolated and cultured as described ( 85 ). For in vitro decidualization, stromal cells were treated with estradiol-17β (10 nM) and progesterone (1 μM) in Dulbecco’s modified Eagle’s medium/F12 containing 2% charcoal-treated fetal bovine serum (Biological Industries, USA) ( 84 ).…”

Section: Methodsmentioning

confidence: 99%

IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice

Yan

et al. 2023

Sci. Signal.

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The establishment of pregnancy depends on interactions between the epithelial and stromal cells of the endometrium that drive the decidual reaction that remodels the stroma and enables embryo implantation. Decidualization in mice also depends on ovarian hormones and the presence of a blastocyst. Hedgehog signaling is transduced by primary cilia in many tissues and is involved in epithelial-stromal cross-talk during decidualization. We found that primary cilia on mouse uterine stromal cells increased in number and length during early pregnancy and were required for decidualization. In vitro and in vivo, progesterone promoted stromal ciliogenesis and the production of Indian hedgehog (IHH) in the epithelium and Sonic hedgehog (SHH) in the stroma. Blastocyst-derived TNF-α also induced epithelial IHH, which stimulated the production of SHH in the stroma through a mechanism that may involve the release of arachidonic acid from epithelial cells. In the stroma, SHH activated canonical Hedgehog signaling through primary cilia and promoted decidualization through a mechanism that depended on interleukin-11 (IL-11) and primary cilia. Our findings identify a primary cilia–dependent network that controls endometrial decidualization and suggest primary cilia as a candidate therapeutic target for endometrial diseases.

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Section: Methodsmentioning

confidence: 99%

IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice

Yan

et al. 2023

Sci. Signal.

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“…Western blot was carried out as previously stated [ 69 ]. Briefly, cultured cells were lysed in lysis buffer (50 mM Tris-HCl, pH 7.5; 150 mM NaCl; 0.25% sodium deoxycholate and 1% Triton X-100).…”

Section: Methodsmentioning

confidence: 99%

Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization

Yan

Chen

et al. 2022

IJMS

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Endometrial decidualization plays a pivotal role during early pregnancy. Compromised decidualization has been tightly associated with recurrent implantation failure (RIF). Primary cilium is an antenna-like sensory organelle and acts as a signaling nexus to mediate Hh, Wnt, TGFβ, BMP, FGF, and Notch signaling. However, whether primary cilium is involved in human decidualization is still unknown. In this study, we found that primary cilia are present in human endometrial stromal cells. The ciliogenesis and cilia length are increased by progesterone during in vitro and in vivo decidualization. Primary cilia are abnormal in the endometrium of RIF patients. Based on data from both assembly and disassembly of primary cilia, it has been determined that primary cilium is essential to human decidualization. Trichoplein (TCHP)-Aurora A signaling mediates cilia disassembly during human in vitro decidualization. Mechanistically, primary cilium modulates human decidualization through PTEN-PI3K-AKT-FOXO1 signaling. Our study highlights primary cilium as a novel decidualization-related signaling pathway.

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“…In addition to increased senescence during decidualization, in vitro embryo implantation in mice differs from that in humans in specific ways [45, 51, 52]. Caveolin 1 (CAV1), a protein required for senescence in various organs, shows an opposite pattern between mice and humans [51].…”

Section: Physiological Cell Senescence At the Maternal–fetal Interfacementioning

confidence: 99%

“…Caveolin 1 (CAV1), a protein required for senescence in various organs, shows an opposite pattern between mice and humans [51]. CAV1 is significantly upregulated in mice, whereas it is downregulated during human decidualization, owing to the activation of the cAMP pathway that is activated during the process [52].…”

Section: Physiological Cell Senescence At the Maternal–fetal Interfacementioning

confidence: 99%

“…Oxidative stress occurs when the balance between redox reactions and antioxidants is altered. Redox imbalance at the maternal–fetal interface is associated with AMA pregnancy and pregnancy abnormalities [52, 53]. Examination of the uterine redox status in young and reproductively aged female mice revealed a redox imbalance at the maternal–fetal interface in reproductively aged mice at 20.5 days of gestation.…”

Section: Advanced Maternal Age and Excessive Cell Senescence At The M...mentioning

confidence: 99%

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Flip a coin: cell senescence at the maternal–fetal interface

Gong

Muyayalo

Zhang

et al. 2023

Biology of Reproduction

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During pregnancy, cell senescence at the maternal-fetal interface is required for maternal well-being, placental development, and fetal growth. However, recent reports have shown that aberrant cell senescence is associated with multiple pregnancy-associated abnormalities, such as preeclampsia, fetal growth restrictions, recurrent pregnancy loss, and preterm birth. Therefore, the role and impact of cell senescence during pregnancy requires further comprehension. In this review, we discuss the principal role of cell senescence at the maternal-fetal interface, emphasizing its “bright side” during decidualization, placentation, and parturition. Additionally, we highlight the impact of its deregulation and how this “dark side” promotes pregnancy-associated abnormalities. Furthermore, we discuss novel and less invasive therapeutic practices associated with the modulation of cell senescence during pregnancy.

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Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization

Cited by 11 publications

References 58 publications

IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice

IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice

Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization

Flip a coin: cell senescence at the maternal–fetal interface

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