Caveolin-1–eNOS/Hsp70 interactions mediate rosuvastatin antifibrotic effects in neonatal obstructive nephropathy

García, Isabel Mercedes; Mazzei, Luciana; Benardón, María Eugenia; Oliveros, Liliana; Cuello-Carrión, F. Darío; Lorenzo, Andrea Gil; Manucha, Walter; Vallés, Patricia G.

doi:10.1016/j.niox.2012.05.006

Cited by 12 publications

(12 citation statements)

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“…Kidney paraffin sections were processed according to previously described technique with minor modifications [ 21 ]. The antibodies applied were rabbit polyclonal antibody against WT-1 (C-19), mouse monoclonal antibody against VDR (D-6), rabbit polyclonal antibody against Hsp70 (H-300) and rabbit polyclonal antibody against AT 1 (306) (Santa Cruz Biotechnology, Inc.), diluted at 1:500.…”

Section: Methodsmentioning

confidence: 99%

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Changes in renal WT-1 expression preceding hypertension development

et al. 2016

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BackgroundHypertension is a public health problem with mostly unknown causes, and where strong hereditary genetic alterations have not been fully elucidated. However, the use of experimental models has provided valuable information. Recent evidences suggest that alterations in key nephrogenic factors, such as Wilms’ tumor 1 transcription factor (WT-1), could contribute to the development of hypertension. The aim of this paper is to evaluate the expression of WT-1 and related genes in the nephrogenic process in connection with the development of hypertension as well as the corresponding anatomical and functional correlation.MethodsMale spontaneously hypertensive and control rats were evaluated weekly from birth until week 8 of life. Their blood pressure was taken weekly using the tail-cuff blood pressure system. Weekly, 5 rats per group were sacrificed with a lethal injection of pentobarbital, and their kidneys were removed, decapsulated and weighed. The serum was collected for measuring biochemical parameters. The results were assessed using one-way analysis of variance for comparisons between groups.ResultsThe relationship between renal weight/total body weights was established, without significantly different values. These data were compared with apoptosis, fibrosis, number and size of the glomeruli. The elevation of systolic blood pressure was significant since week 6. Biochemical values differed slightly. Histology showed a slight increase in deposits of collagen fibers since week 4. Additionally, in kidney cortices, the expression of WT-1, heat shock protein 70 (Hsp70) and vitamin D receptors (VDR) decreased since week 4. Finally, we demonstrated ultrastructural damage to mitochondria since week 4.ConclusionsOur results would suggest an unprecedented link, possibly a regulatory mechanism, between WT-1 on nephrogenic alteration processes and their relationship with hypertension. Moreover, and previous to the increase in blood pressure, we demonstrated low expressions of WT-1, VDR and Hsp70 in kidneys from neonatal SHRs. If so, this may suggest that deregulation in the expression of WT-1 and its impact on nephrogenesis induction could be crucial in understanding the development and maintenance of hypertension.Electronic supplementary materialThe online version of this article (doi:10.1186/s12882-016-0250-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

“…The kidneys were processed according to previously described technique [ 21 ]. Primary antibodies were: polyclonal anti-WT-1 (1:100) (Santa Cruz Biotechnology), monoclonal anti-Hsp70 (1:100) (Sigma Aldrich), monoclonal anti-VDR (1:100) (Santa Cruz Biotechnology), and polyclonal anti-AT 1 (1:100) (Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

Changes in renal WT-1 expression preceding hypertension development

et al. 2016

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“…Glucose-6-phosphatase level was the most decreased in comparison with other analyzed enzymes. Glucose homeostasis in the liver consists of a number of mechanisms, but low release study where downregulation of caveolin-1 related to upregulation of eNOS/HSP70 in tubulointerstitial fibrosis injury in neonatal early kidney obstruction treated with rosuvastatin [14]. HSP70 liver concentration can be higher in IT group due to increased expression, which was not assessed in this experiment [15].…”

Section: Discussionmentioning

confidence: 84%

Ileal transposition in rats influenced glucose metabolism and HSP70 levels

et al. 2015

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Objective: Ileal transposition procedure (IT), in combination with sleeve gastrectomy, is widely used to induce diabetes remission and to control related metabolic abnormalities. A transposition of a long segment of distal ileum in obese Zucker rats improved glucose tolerance 6 months after IT. The premise of our study was to to examine the long - term effects of ileum transposition on the liver glycolytic enzymes content in a euglycemic group of operated Zucker rats. Methods: Twenty male Zucker rats underwent either the transposition of 50% distal ileum or a sham surgery. Six months after surgery, liver tissue concentrations of glycogen synthase kinase alpha (GSK-3α), glucose 6-phosphatase (G6PC), glycogen phosphorylase (PYGM) and phosphofructokinase (PFK) and HSP70 were assessed by immunoenzymatic methods. Results: HSP70 values were significantly higher in the IT group compared to SHAM. G6PC liver concentrations in the IT group were almost 1.45-fold lower than in the SHAM operated rats. Statistical analyses (F-test) showed HSP70 levels were significantly related to caveolin-1and SHAM group. Conclusions: Lowered glycolytic enzyme concentrations assessed in the liver suggest positive effects on glucose metabolism in long-term observations.

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“…The kidney and heart paraffin sections were processed according to a previously described technique . The antibodies applied were rabbit polyclonal antibody against AT 1 (306), rabbit polyclonal antibody against Hsp70 (H‐300), and mouse monoclonal antibody against VDR (D‐6) (Santa Cruz Biotechnology, Inc.), each of which was diluted at 1:500.…”

Section: Methodsmentioning

confidence: 99%

Antiarrhythmic effect linked to melatonin cardiorenal protection involves AT₁ reduction and Hsp70‐VDR increase

Prado

Casarotto

Calvo

et al. 2018

Journal of Pineal Research

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Lethal ventricular arrhythmias increase in patients with chronic kidney disease that suffer an acute coronary event. Chronic kidney disease induces myocardial remodeling, oxidative stress, and arrhythmogenesis. A manifestation of the relationship between kidney and heart is the concomitant reduction in vitamin D receptor (VDR) and the increase in angiotensin II receptor type 1 (AT ). Melatonin has renal and cardiac protective actions. One potential mechanism is the increase in the heat shock protein 70 (Hsp70)-an antioxidant factor. We aim to determine the mechanisms involved in melatonin (Mel) prevention of kidney damage and arrhythmogenic heart remodeling. Unilateral ureteral-obstruction (UUO) and sham-operated rats were treated with either melatonin (4 mg/kg/day) or vehicle for 15 days. Hearts and kidneys from obstructed rats showed a reduction in VDR and Hsp70. Associated with AT up-regulation in the kidneys and the heart of UUO rats also increased oxidative stress, fibrosis, apoptosis, mitochondrial edema, and dilated crests. Melatonin prevented these changes and ventricular fibrillation during reperfusion. The action potential lengthened and hyperpolarized in melatonin-treated rats throughout the experiment. We conclude that melatonin prevents renal damage and arrhythmogenic myocardial remodeling during unilateral ureteral obstruction due to a decrease in oxidative stress/fibrosis/apoptosis associated with AT reduction and Hsp70-VDR increase.

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Caveolin-1–eNOS/Hsp70 interactions mediate rosuvastatin antifibrotic effects in neonatal obstructive nephropathy

Cited by 12 publications

References 40 publications

Changes in renal WT-1 expression preceding hypertension development

Changes in renal WT-1 expression preceding hypertension development

Ileal transposition in rats influenced glucose metabolism and HSP70 levels

Antiarrhythmic effect linked to melatonin cardiorenal protection involves AT₁ reduction and Hsp70‐VDR increase

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Caveolin-1–eNOS/Hsp70 interactions mediate rosuvastatin antifibrotic effects in neonatal obstructive nephropathy

Cited by 12 publications

References 40 publications

Changes in renal WT-1 expression preceding hypertension development

Changes in renal WT-1 expression preceding hypertension development

Ileal transposition in rats influenced glucose metabolism and HSP70 levels

Antiarrhythmic effect linked to melatonin cardiorenal protection involves AT1 reduction and Hsp70‐VDR increase

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Antiarrhythmic effect linked to melatonin cardiorenal protection involves AT₁ reduction and Hsp70‐VDR increase