Ileal transposition in rats influenced glucose
metabolism and HSP70 levels

Stygar, Dominika; Sawczyn, Tomasz; Skrzep-Poloczek, Bronisława; Karcz-Socha, Iwona; Doleżych, Bogdan; Zawisza-Raszka, Agnieszka; Augustyniak, Maria; Żwirska-Korczala, K; Karcz, Wojciech Konrad

doi:10.1515/biol-2015-0029

Cited by 5 publications

(3 citation statements)

References 19 publications

(30 reference statements)

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“…The obtained results suggest that the combination of DJOS and a change of the diet was a strong stimulus for HSP70 level induction in plasma and liver tissue. In our previous study, we reported increased HSP70 plasma levels after the ileal transposition (IT) in comparison to SHAM-operated, diabetic fatty Zucker rats (Stygar et al 2015 ). Angelini and co-workers showed that duodenal-jejunal bypass surgery leads to a decrease in levels of plasma HSP70 in obese, insulin-resistant (IR) and nonalcoholic fatty liver disease (NAFLD) subjects (Angelini et al 2018 ).…”

Section: Discussionmentioning

confidence: 96%

The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats

Stygar

Skrzep-Poloczek

Romuk

et al. 2019

Cell Stress and Chaperones

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Metabolic surgery ameliorates insulin resistance and is associated with long-term, effective weight loss, but the mechanisms involved remain unknown. Here, the duodenal-jejunal omega switch (DJOS) surgery in combination with high-fat, high-carbohydrate diet was performed on diet obese rats and joint effects of bariatric surgery and different dietary patterns on heat shock protein 70 (HSP70) and HSP90 plasma and liver concentrations were measured. We found that plasma and liver levels of HSP70 were lower after DJOS surgery in comparison to the control in the groups of animals kept on control diet (CD) and high-fat, high-sugar diet (HFS) but the postoperative change of the diet led to the increase in HSP70 in plasma and liver concentration in DJOS-operated animals. A high-calorie meal, rich in carbohydrates and fats, significantly increased circulating levels of HSP90, reducing the normalising effect of DJOS. The HFS diet applied during all stages of the experiment led to the higher levels of liver HSP90 concentration. The combination of CD and DJOS surgery was the most efficient in the lowering of the HSP90 liver concentration. The normalisation of circulating levels and liver concentrations of HSP70 and HSP90 may be achieved in a combination of DJOS procedure with a proper dietary plan.

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Section: Discussionmentioning

confidence: 96%

The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats

Stygar

Skrzep-Poloczek

Romuk

et al. 2019

Cell Stress and Chaperones

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“…and Stygar et al . 43 , 59 . Briefly, 2% isoflurane with 2 l/min oxygen flow under spontaneous breathing was used to induce and maintain anaesthesia; analgesia was performed with xylazine (5 mg/kg b.w., ip; Xylapan, Vetoquinol Biovet, Poland) and antibiotic prophylaxis with gentamicin (5 mg/kg b.w., im, KRKA, Poland).…”

Section: Methodsmentioning

confidence: 99%

Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl:ZUC(ORL)-Leprfa) rats

Stygar

Sawczyn

Dulska

et al. 2021

Sci Rep

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We studied the long-term effect of ileal transposition (IT) metabolic surgery on the hepatokines: retinol-binding protein-4 (RBP4), α-2-HS-glycoprotein (aHSG/fetuin-A), and fibroblast growth factor 21 (FGF21), C-reactive protein (CRP) plasma levels, glucose metabolism, body weight, liver histology, as well as total lipids concentration in muscle, liver, and fat tissue of obese Zucker (Crl:ZUC(ORL)-Leprfa) rats. 14 adult males were randomly submitted either to IT or SHAM (control) surgery. Pre-operative hepatokines plasma levels were not significantly different in rats submitted to IT or SHAM protocol. Three months after the procedures the plasma levels of RBP4, aHSG, FGF21, and CRP were significantly lower in IT-operated animals when compared to SHAM-operated group. Three and 12 weeks after the IT and SHAM surgery, the AUCOGTT were significantly lower than AUCOGTT before the surgery. HOMA-IR was lower in rats after IT surgery in comparison to the SHAM-operated rats. Muscle and liver total lipids concentration was reduced after the IT procedure when compared to pre-IT conditions. IT had a significant reductive impact on the body weight in comparison to SHAM surgery in the 4th, 6th, 8th, and 10th week after the surgery. We conclude that IT reduces hepatokines’ plasma concentrations, muscle and liver total lipids concentration but not the inflammatory processes in the liver of Zucker (Crl:ZUC(ORL)-Leprfa) rats.

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“…For RYGBP, the gastric bypass model was established following a previously described protocol (23). For IT surgery, Treitz's ligament was identified, the jejunum was divided 5 cm aborally and the ileal loop was the interpositioned in an isoperistaltic fashion, forming two end-to-end anastomoses (24). For the combination of RYGBP and IT surgery, Treitz's ligament was identified, the jejunum was divided 5 cm aborally and anastomoses were formed in the distal jejunum and proximal.…”

Section: Animalsmentioning

confidence: 99%

Effect of gastric bypass combined with ileal transportation on type 2 diabetes mellitus

et al. 2018

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Abstract. Type 2 diabetes mellitus (T2DM) is a chronic progressive disease, which manifests as an endocrine disorder. Among the different methods of surgery available to treat patients with T2DM, Roux-en-Y gastric bypass (RYGBP) and ileal transposition (IT) are the most commonly performed. The aim of the present study was to investigate the effects of RYGBP combined with IT on rats with T2DM. A total of 8 healthy male rats were used as a control group and 40 GK rats were randomly divided into 5 groups: A diabetes mellitus (DM) group, a sham operative group (SO), a RYGBP group, an IT group and a RYGBP+IT group. The results demonstrated that fasting blood glucose, triglyceride, total cholesterol and gastric inhibitory polypeptide levels in all treatment groups were significantly lower than those of the SO and DM groups. Furthermore, levels TC and TG in the RYGBP+IT group were significantly lower than in the RYGBP and IT groups. Levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase mRNA and IRS-2 protein in all treatment groups were also significantly lower than those of the SO group; and they were significantly lower in the RYGBP+IT group compared with the RYGBP and IT groups. The expression of phosphorylated Akt in the treatment groups was significantly higher than the SO group and was significantly higher in the RYGBP+IT group compared with the RYGBP and IT groups. These results indicate that RYGBP and IT surgical treatment can induce T2DM remission by mediating the expression of insulin-related factors to reverse insulin resistance. The current study also indicated that the effect of RYGBP combined with IT may be developed as a novel first-line method of treating T2DM.

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Ileal transposition in rats influenced glucose metabolism and HSP70 levels

Cited by 5 publications

References 19 publications

The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats

The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats

Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl:ZUC(ORL)-Leprfa) rats

Effect of gastric bypass combined with ileal transportation on type 2 diabetes mellitus

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