Cave Cyanobacteria showing antibacterial activity

Lamprinou, Vasiliki; Tryfinopoulou, Kyriaki; Velonakis, Emmanuel; Vatopoulos, Alkiviadis; Antonopoulou, Smaragdi; Fragopoulou, Elizabeth; Pantazidou, Adriani; Economou‐Amilli, Athena

doi:10.5038/1827-806x.44.3.2

Cited by 24 publications

(28 citation statements)

References 39 publications

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“…Considering the last-mentioned approach, caves represent one of the most attractive environments with a strong potential for the discovery of novel antimicrobials. Antimicrobial activity of different cave microorganisms against pathogenic bacteria was reported previously (Herold et al, 2005;Nakaew et al, 2009;Rajput et al, 2012;Cheeptham et al, 2013;Rule & Cheeptham, 2013;Tomova et al, 2013;Lamprinou et al, 2015); a new genus of bacteria, producing antimicrobial substances, was identified in a cave in Thailand (Nakaew et al, 2012).…”

Section: Introductionmentioning

confidence: 70%

“…Antibiotic resistance of pathogenic bacteria became one of the greatest threats to human health over the last few decades (Blair et al, 2015). A few different approaches are used to resolve this problem: chemical synthesis of novel antibiotics (Wright et al, 2014), discovery and development of novel agents capable of circumventing or neutralizing the existing resistance mechanisms (Blair et al, 2015), as well as search for new natural antimicrobials (Lamprinou et al, 2015). Considering the last-mentioned approach, caves represent one of the most attractive environments with a strong potential for the discovery of novel antimicrobials.…”

Section: Introductionmentioning

confidence: 99%

“…Antimicrobial potential of cave actinobacteria and especially Streptomyces was investigated (Herold et al, 2005;Nakaew et al, 2009;Hodges et al, 2012;Rajput et al, 2012;Cheeptham et al, 2013;Rule & Cheeptham, 2013;Nimaichand et al, 2015), but the antimicrobial compounds of bacteria from other phyla -Cyanobacteria (Lamprinou et al, 2015), Proteobacteria and Bacteroidetes (Tomova et al, 2013) -were also of scientific interest. Our previous cultivation-independent analysis of bacterial diversity in Krubera-Voronja Cave showed that Gram-positive phyla Actinobacteria and Firmicutes were among the most numerous phyla in this cave (Kieraite-Aleksandrova et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of antimicrobial activity of culturable bacteria isolated from Krubera-Voronja Cave

Klusaite¹,

Vickackaite²,

Vaitkeviciene³

et al. 2016

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In the present study we aimed to perform the first analysis of antimicrobial activity of bacteria isolated from Krubera-Voronja Cave, with the main focus on their activity against Grampositive bacteria, including Gram-positive pathogens. Using five different media, in total 874 heterotrophic cultures were isolated from water and sediment samples collected in Krubera-Voronja Cave at a depth from 220 m to 1640 m. 14.0% of all isolates demonstrated antibacterial activity against Gram-positive and Gram-negative test microorganisms. Our results show that this percentage was not uniform; it increased with the sampling depth and was the highest in the lower part of the cave. 24 isolates were active exclusively against Gram-positive test strains Micrococcus luteus and Bacillus thuringiensis. Two isolates, namely strains 1350R2-TSA30-6 and 1410WF1-TSA30-2, were chosen for the further work because of the high and comparable activity against both Gram-positive test microorganisms. It was determined that both strains belong to the family Bacillaceae in phylum Firmicutes. The detailed bioactivity analysis of these two Gram-positive strains revealed the different mixtures of volatile compounds with antibacterial activity. The main antibacterial compounds of the strain 1350R2-TSA30-6 are pyrrolopyrazines pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-and pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-. The main antibacterial compound of the strain 1410WF1-TSA30-2 is 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester. Mixtures of the volatile antimicrobial compounds of both strains were antagonistic against Gram-positive strains isolated from Krubera-Voronja Cave, and their activity against Gram-positive pathogenic bacteria substantially differed. antimicrobial, Bacillus, cave microorganisms, Krubera-Voronja Cave, volatile compound

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Section: Introductionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of antimicrobial activity of culturable bacteria isolated from Krubera-Voronja Cave

Klusaite¹,

Vickackaite²,

Vaitkeviciene³

et al. 2016

IJS

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“…Nevertheless, Groth & SaizJimenez (1999) have suggested that actinomycetes are abundant in caves and their growth is favoured by the association of low temperature and high relative humidity. Moreover, algae and cyanobacteria have been commonly found in caves (e.g., Banfield et al, 1999;Roldán & Hernández-Mariné, 2009;Lamprinou et al, 2015), whereas Laiz et al, (2000) sampled many bacterial isolates from speleothems, and more specifically from stalactites, which include various species of Streptomyces, Bacillus, Nocardiopsis, Rhodococcus, Agromyces, Arthrobacter, Amycolatopsis, Brevibacillus, Micrococcus, Staphylococcus, Paenibacillus and Variovorax. Therefore, in the microenvironments within stalactites, the metabolic activities of such microorganisms may have locally lowered the pH enough to promote the alteration of muscovite in the Agios Georgios stalactite.…”

Section: Implications For Microbial Muscovite Dissolutionmentioning

confidence: 99%

A new microenvironment for the formation of clay minerals: the example of authigenic halloysite-7Å and gibbsite in a stalactite from Agios Georgios Cave, Kilkis, north Greece

Ifandi¹,

Tsikouras²,

Papoulis³

et al. 2015

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Abstract:An unusual authigenic origin for halloysite and gibbsite is reported in a stalactite from Agios Georgios Cave, Kilkis. This speleothem includes mostly pure calcite whereas minor areas of Mg-rich calcite and scarce dolomite are present in four growth phases. Abundant pores are created due to imperfect coalescence of the calcite crystals. Several of them contain detrital muscovite, which was presumably transferred from the dripping water, during the formation of speleothem and has been variably altered to halloysite. Several pores in the stalactite contain different mineral assemblages that we interpret as in situ: halloysite-7Å, halloysite + silica, gibbsite + silica and gibbsite. The breakdown of the muscovite and the formation of halloysite require acidic conditions, which we suggest to have been established by potassium solubilising microorganisms. The silica minerals include spheroidal assemblages or needlelike and blade-like quartz and can be explained by further dissolution of halloysite, under the same acidic conditions in the presence of microorganisms. In our model, the precipitation of gibbsite is the result of direct formation from muscovite, promoted from abundant and undisturbed water percolation, at moderately low pH, also induced by the presence of bacteria. Given that microbial activities promote: (1) breakdown of muscovite and formation of halloysite, silica, and gibbsite, and (2) formation of Mg-calcite and dolomite after calcite, then it is likely that two or more different microbial communities may exist in the same speleothem. The first creates mild acidic conditions, aiming at the decomposition of muscovite in the microenvironment of the pores antagonising the second that produces alkaline microregimes and the local precipitation of Mg-rich carbonate minerals.

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“…Cyanobacteria are an interesting source of natural bioactive compounds (Chlipala et al 2011) that possess antibacterial (Gantar et al 2011;Lamprinou et al 2015), antimycotic (Swain et al 2017) and anticancer (Kinghorn et al 2016) activity. Terrestrial cyanobacteria, particularly, form an interesting subgroup of previously uninvestigated diversity (Garcia-Pichel et al 2001).…”

Section: Introductionmentioning

confidence: 99%

A qPCR method for distinguishing biomass from non-axenic terrestrial cyanobacteria cultures in hetero- or mixotrophic cultivations

et al. 2020

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The cultivation of cyanobacteria with the addition of an organic carbon source (meaning as heterotrophic or mixotrophic cultivation) is a promising technique to increase their slow growth rate. However, most cyanobacteria cultures are infected by non-separable heterotrophic bacteria. While their contribution to the biomass is rather insignificant in a phototrophic cultivation, problems may arise in heterotrophic and mixotrophic mode. Heterotrophic bacteria can potentially utilize carbohydrates quickly, thus preventing any benefit for the cyanobacteria. In order to estimate the advantage of the supplementation of a carbon source, it is essential to quantify the proportion of cyanobacteria and heterotrophic bacteria in the resulting biomass. In this work, the use of quantitative polymerase chain reaction (qPCR) is proposed. To prepare the samples, a DNA extraction method for cyanobacteria was improved to provide reproducible and robust results for the group of terrestrial cyanobacteria. Two pairs of primers were used, which bind either to the 16S rRNA gene of all cyanobacteria or all bacteria including cyanobacteria. This allows a determination of the proportion of cyanobacteria in the biomass. The method was established with the two terrestrial cyanobacteria Trichocoleus sociatus SAG 26.92 and Nostoc muscorum SAG B-1453-12a. As proof of concept, a heterotrophic cultivation with T. sociatus with glucose was performed. After 2 days of cultivation, a reduction of the biomass partition of the cyanobacterium to 90% was detected. Afterwards, the proportion increased again.

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Cave Cyanobacteria showing antibacterial activity

Abstract: Abstract:al., Engene et al., 2013). These interesting and biochemically active compounds possess biological activity covering a wide range of antibacterial (Mundt et al

Cited by 24 publications

References 39 publications

Characterization of antimicrobial activity of culturable bacteria isolated from Krubera-Voronja Cave

Characterization of antimicrobial activity of culturable bacteria isolated from Krubera-Voronja Cave

A new microenvironment for the formation of clay minerals: the example of authigenic halloysite-7Å and gibbsite in a stalactite from Agios Georgios Cave, Kilkis, north Greece

A qPCR method for distinguishing biomass from non-axenic terrestrial cyanobacteria cultures in hetero- or mixotrophic cultivations

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