Caution of intensified spread of antibiotic resistance genes by inadvertent introduction of beneficial bacteria into soil

Kang, Yijun; Shen, Min; Xia, Dan; Ye, Kaixia; Qingxin, Zhao; Hu, Jian

doi:10.1080/09064710.2017.1314548

Cited by 16 publications

(21 citation statements)

References 64 publications

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“…Moreover, the rhizosphere is regarded as a hot spot of microbial activity and gene transfer: hence, ARB can transfer ARGs, often located on mobile genetic elements, to other bacteria inhabiting soil and plant, threatening animal and human health ( Blau et al, 2019 ). We believe that it is crucial to avoid large-scale deliberate introduction of beneficial bacteria into soil, which could exacerbate the spread of ARGs in the environment ( Kang et al, 2017 ). We tested the resistance of the five bacterial strains to six antibiotics that span different mechanisms of action, finding out that antibiotic resistance is poorly detectable in these strains.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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Global population growth and climate change raise a challenge to agriculture, which, combined with the issues concerning the use of chemical fertilizers, have generated increasing attention in the use of plant-associated bacteria as a sustainable strategy in agri-food systems. The objective of this study is to evaluate the ability of five bacterial strains, previously isolated from the rhizosphere or endosphere of plants adapted to harsh environmental conditions, to act as potential plant biofertilizers in different conditions of water availability. The strain biosafety for a deliberate environmental release was investigated through a literature survey and antibiotic resistance testing. The selected strains were first characterized for their plant growth–promoting (PGP) and rhizocompetence-related traits through in vitro assays and then on short-term in vivo experiments on tomato plants. A long-term greenhouse experiment was further conducted to monitor the PGP effect of the bacteria during the entire life cycle of tomato plants subjected to full irrigation or to severe water deficit conditions, aiming to assess their actual effect on plant productivity, which is the ultimate target of the agricultural sector. Some of the strains showed a potential in improving water use efficiency and mitigating plant water stress. Under severe irrigation deficit, four of the tested strains, Micrococcus yunnanensis M1, Bacillus simplex RP-26, Pseudomonas stutzeri SR7-77, and Paenarthrobacter nitroguajacolicus 2–50, significantly increased the number of productive plants in comparison to non-bacterized control ones. Two of them, Bacillus simplex RP-26 and Paenarthrobacter nitroguajacolicus 2–50, demonstrated also, under full irrigation, to significantly improve the water productivity in comparison with non-bacterized plants. Despite all the strains showed promising PGP potential in short-term assays, the positive effect of the bacterial inoculants on plant physiology and fruit yield was observed in some cases but never corroborated by statistical significance. These results highlight the importance of performing long-term in vivo experiments to define the real PGP ability of a bacterial inoculant to positively impact plant production.

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Section: Discussionmentioning

confidence: 99%

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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“…A potential PGPB should: be identified as a taxonomical unit univocally, be effective against target phytopathogens, not show clinical or animal toxicity, and not persist in the agro-environment. Moreover, PGPB should not transfer its genetic material to other closely related microbes to avoid the risk of antibiotic resistance development [ 226 ]. With these, the antagonistic potential and behavioral features of a potential PGPB must be thoroughly characterized to permit its registration as a biocontrol agent and approval for use in plant protection.…”

Section: Bioformulations Of Pgpb In Rice: Applications Challenges and Future Prospectsmentioning

confidence: 99%

Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

et al. 2021

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As a major food crop, rice (Oryza sativa) is produced and consumed by nearly 90% of the population in Asia with less than 9% produced outside Asia. Hence, reports on large scale grain losses were alarming and resulted in a heightened awareness on the importance of rice plants’ health and increased interest against phytopathogens in rice. To serve this interest, this review will provide a summary on bacterial rice pathogens, which can potentially be controlled by plant growth-promoting bacteria (PGPB). Additionally, this review highlights PGPB-mediated functional traits, including biocontrol of bacterial rice pathogens and enhancement of rice plant’s growth. Currently, a plethora of recent studies address the use of PGPB to combat bacterial rice pathogens in an attempt to replace existing methods of chemical fertilizers and pesticides that often lead to environmental pollutions. As a tool to combat bacterial rice pathogens, PGPB presented itself as a promising alternative in improving rice plants’ health and simultaneously controlling bacterial rice pathogens in vitro and in the field/greenhouse studies. PGPB, such as Bacillus, Pseudomonas, Enterobacter, Streptomyces, are now very well-known. Applications of PGPB as bioformulations are found to be effective in improving rice productivity and provide an eco-friendly alternative to agroecosystems.

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“…Contact with heavy metals may co-select antibiotic resistant bacteria (ARB) [89], which is one of the major neglected issues in deploying bacterial-based biofertilizers. Many PGPR biofertilizers harbor more than one plasmid and chromosome-borne antibiotic resistance genes (ARGs) [90,91]. Hence, the potential risks of transferring ARGs to neighboring bacteria and even to crops [90] is a major challenge.…”

Section: Antibiotic Salt Heavy Metal and Rare Elements Resistance mentioning

confidence: 99%

Rhizospheric Phosphate Solubilizing Bacillus atrophaeus GQJK17 S8 Increases Quinoa Seedling, Withstands Heavy Metals, and Mitigates Salt Stress

et al. 2021

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Introduction of quinoa (Chenopodium quinoa willd.), a gluten-free nutritious pseudo-cereal, outside its traditional growing areas exposed it to seedling damping-off. Here, we isolated eleven phosphate-solubilizing bacteria from the quinoa rhizosphere and assessed their effect on germination and seedlings growth. All isolates solubilized phosphate, produced indole3-acetic acid, hydrocyanic acid, siderophores, and ammonia. Genotypic analysis revealed that our strains are related to the genus of Bacillus, Pseudomonas, and Enterobacter. Strains Enterobacter asburiae (QD14, QE4, QE6, and QE16), Enterobacter sp. QE3, and Enterobacter hormaechei QE7 withstood 1.5 mg·L−1 of cadmium sulfate, 0.5 mg·mL−1 of nickel nitrate, and 1 mg·mL−1 of copper sulfate. Moreover, all strains solubilized zinc from ZnO; P. Stutzeri QD1 and E. asburiae QD14 did not solubilize Zn3(PO4)2 and CO3Zn, whereas CO3Zn was not solubilized by E. asburiae QE16. Bacillus atrophaeus S8 tolerated 11% NaCl. P. frederiksbergensis S6 and Pseudomonas sp. S7 induced biofilm formation. Anti-fusarium activity was demonstrated for E.asburiae QE16, P. stutzeri QD1, P. frederiksbergensis S6, Pseudomonas sp. S7, and B. atrophaeus S8. Lastly, inoculation of quinoa seeds with B. atrophaeus S8 and E. asburiae QB1 induced the best germination rate and seedling growth, suggesting their potential use as inoculants for salty and heavy metal or zinc contaminated soils.

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Caution of intensified spread of antibiotic resistance genes by inadvertent introduction of beneficial bacteria into soil

Cited by 16 publications

References 64 publications

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

Rhizospheric Phosphate Solubilizing Bacillus atrophaeus GQJK17 S8 Increases Quinoa Seedling, Withstands Heavy Metals, and Mitigates Salt Stress

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