Cattle fetal sex determination by polymerase chain reaction using DNA isolated from maternal plasma

Cruz, Alex Silva da; Silva, Danilo Conrado; Costa, Emília Oliveira Alves; M-Jr., P. De; Silva, C.C. da; Silva, D.M.

doi:10.1016/j.anireprosci.2012.02.004

Cited by 17 publications

(10 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serum from 14 rhinoceroses pregnant with 7 male and 7 female fetuses during 61–490 days of gestation were analyzed by PCR‐based sexing, and corroborated the sex of each delivered calf following a reamplification step for SRY . Similarly high levels of accuracy were documented for other PCR‐based assays in maternal serum from bovine (da Cruz et al, ; Davoudi et al, ; Lemos et al, ; Wang et al, ), ovine (Kadivar et al, ; Saberivand & Ahsan, ), and equine (de Leon et al, ; Kadivar et al, ). The earliest that fetal sex determination was made was 61 (female fetus) and 72 (male fetus) days of gestation, which is the timeframe when fetal sex can be determined via rectal ultrasound in all rhinoceros species (Radcliffe et al, , ; Roth et al, ; Stoops et al, ).…”

Section: Discussionsupporting

confidence: 54%

“…Determining fetal sex using sex‐specific DNA sequences in maternal serum by PCR is successfully performed in species with varied types of placentation and hence different degrees of maternal fetal contact—including horses (de Leon et al, ; Kadivar et al, ), cattle (da Cruz et al, ; Davoudi et al, ; Lemos et al, ; Wang et al, ), sheep (Asadpour, Asadi, Jafari‐Joozani, & Hamidian, ; Kadivar et al, ), humans (Lo et al, ), and primates (Jimenez & Tarantal, ; Yasmin, Takano, Nagai, Otsuki, & Sandai, ). SRY (Sex‐determining region Y) is the most commonly used gene for sex typing in mammals, particularly when an internal control is included to detect a gene present on the X chromosome as validation of the amplification process.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

Stoops

Winget

DeChant

et al. 2018

Molecular Reproduction Devel

View full text Add to dashboard Cite

Genetic sexing of animals with long gestation time benefits the management of captive populations. Here, X and Y chromosome-specific primers, based on equine gene sequencing data, were developed and tested on captive rhinoceroses (10 males, 20 females) representing four species (Diceros bicornis, Certaotherium simum simum, Rhinoceros unicornis, and Dicerorhinus sumatrensis). The Y chromosome-specific primer set targeted SRY (Sex-determining region Y), and amplified a 177-bp product following PCR of DNA extracted from males, but not females, of all species. A primer set based on the equine AMEL (Amelogenin) gene resulted in a 232-bp product following PCR of all rhinoceros species. These gene-specific primer sets were then evaluated for their ability to determine gender in cell-free DNA from rhinoceros serum. Modifications to the original extraction and PCR protocols were required to obtain sufficient DNA quantities from serum, and both DNA yield and PCR amplification were substantially reduced or absent following multiple freeze-thaw cycles of serum. When fresh serum from 14 pregnant rhinoceroses (ultimately bearing seven male and seven female calves), representing four species at different stages of gestation (Days 61-490), were probed in a PCR-based assay, an accuracy of 71% was achieved for male-specific gene detection of SRY, which improved to 100% by including a reamplification step into the protocol. Such early sex determination should be a valuable tool for current management practices as well as future assisted reproduction of rhinoceroses.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

Stoops

Winget

DeChant

et al. 2018

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…The genetic diversity of the Y chromosome was determined as lower than that of autosomal chromosomes (Liu et al, 2003;Hellborg and Ellegren, 2004;Ginja et al, 2009). For prenatal sex determination in cattle, fetal sexes were successful determined at 99.9% after 55 days of pregnancy using circulating fetal DNA (da Cruz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Y chromosome analysis of native Turkish cattle breeds by microsatellite markers

Özşensoy¹,

Kurar²,

Bulut³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosomespecific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method. PCR products were separated by capillary electrophoresis and marker genotypes were determined. A total of 41 different alleles were observed. The mean allele number was 5.86 and mean F IS value was 0.427 for all populations. The INRA189 locus was monomorphic for all populations except for South Anatolian Red. UMN0307 and INRA124 loci were monomorphic in the East Anatolian Red and Anatolian Black populations, respectively. The Anatolian Black population was assigned to its own population at a maximum level (78.26%). In this study, a taurine-specific allele was identified in the INRA124 locus. Zebu-and taurine-specific alleles were observed in BM861, INRA189, and UMN0103 loci. Allele 134 in INRA124 seems to be a specific allele of taurine or zebu. The alleles of the UMN0307 and UMN0504 loci need to be investigated for zebu-or taurine-specific alleles. The resulting neighbor-joining tree and structure analysis suggested that the breeds analyzed are consistent with their modern geographical locations.

show abstract

“…() and da Cruz et al. (), where a similar type of DNA extraction method showed satisfactory test results (sensitivity ranging between 89% and 100%).…”

Section: Discussionmentioning

confidence: 92%

Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production

Ristanić

Stanišić

Maletić

et al. 2018

Reprod Domestic Animals

View full text Add to dashboard Cite

Foetal sex determination using polymerase chain reaction (PCR) in mammals is based on the amplification of gender-specific foetal DNA sequences circulating in maternal blood. The bovine synepitheliochorial placenta does not allow a direct contact between the trophoblast and the maternal blood, resulting in difficult passage of foetal DNA and, consequently, its very small amounts in maternal bloodstream. Circulating cell-free foetal DNA (ccffDNA) encompasses short nucleotide fragments (300-600 bp) in maternal circulation. The aim of this study was to assess this non-invasive method in accurate prenatal sexing in early and late gestational periods in comparison with ultrasound diagnostics. As various DNA isolation and amplification methods were tested, their success in obtaining reliable results was evaluated. Two groups were tested, each consisting of 20 pregnant cows. Blood of a bull and a non-pregnant heifer was the controls. Extraction of foetal DNA was accomplished by three different methods: using tubes with silicone membranes, a single-tube extraction without silicone membranes and phenol-chloroform extraction. Following each extraction method, foetal DNA was amplified using PCR and real-time PCR with both bAML and TSPY primers in a separate reaction. Positive results were obtained only after amplification of foetal DNA extracted with a single-tube extraction kit. In comparison with ultrasound examination results and foetal gender recorded at birth, the sensitivity of the PCR test was 90% in Group I, but the technique failed to detect male foetuses in Group II. The real-time PCR test sensitivity in Group I was 90% and in Group II 91.6%.

show abstract

Cattle fetal sex determination by polymerase chain reaction using DNA isolated from maternal plasma

Cited by 17 publications

References 18 publications

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

Y chromosome analysis of native Turkish cattle breeds by microsatellite markers

Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production

Contact Info

Product

Resources

About