Cationically rendered biopolymer surfaces for high protein affinity support matrices

Mohan, Tamilselvan; Ristić, Tijana; Kargl, Rupert; Doliška, Aleš; Köstler, Stefan; Ribitsch, Volker; Marn, Jure; Spirk, Stefan; Stana‐Kleinschek, Karin

doi:10.1039/c3cc46414h

Cited by 26 publications

(21 citation statements)

References 26 publications

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“…Since many bismuth xanthates are readily soluble in apolar organic solvents, trimethylsilyl cellulose (TMSC) was chosen as polysaccharide due to its excellent solubility in aprotic solvents at high degrees of substitution with TMS groups. TMSC has several further advantages in this context: its solubility can be fine-tuned by the degree of substitution, it is rather light-and thermostable under exclusion of air/moisture (Wolfberger et al, 2015) and it is a good film forming polymer (T. Mohan et al, 2013;Orelma, Filpponen, Johansson, Laine, & Rojas, 2011). Further, the conversion into cellulose proceeds via a gas phase reaction employing vaporous HCl (Ehmann et al, 2015;Eero Kontturi & Lankinen, 2010).…”

Section: Introductionmentioning

confidence: 99%

On the formation of Bi 2 S 3 -cellulose nanocomposite films from bismuth xanthates and trimethylsilyl-cellulose

Reishofer

Ehmann

Amenitsch

et al. 2017

Carbohydrate Polymers

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The synthesis and characterization of bismuth sulfide-cellulose nanocomposite thin films was explored. The films were prepared using organosoluble precursors, namely bismuth xanthates for BiS and trimethylsilyl cellulose (TMSC) for cellulose. Solutions of these precursors were spin coated onto solid substrates yielding homogeneous precursor films. Afterwards, a heating step under inert atmosphere led to the formation of thin nanocomposite films of bismuth sulfide nanoparticles within the TMSC matrix. In a second step, the silyl groups were cleaved off by vapors of HCl yielding bismuth sulfide/cellulose nanocomposite films. The thin films were characterized by a wide range of surface sensitive techniques such as atomic force microscopy, attenuated total reflection infrared spectroscopy, transmission electron microscopy and wettability investigations. In addition, the formation of the nanoparticle directly in the TMSC matrix was investigated in situ by GI-SWAXS using a temperature controlled sample stage.

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Section: Introductionmentioning

confidence: 99%

On the formation of Bi 2 S 3 -cellulose nanocomposite films from bismuth xanthates and trimethylsilyl-cellulose

Reishofer

Ehmann

Amenitsch

et al. 2017

Carbohydrate Polymers

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“…A promising cellulose derivative for the preparation of cellulose thin films is trimethylsilyl cellulose (TMSC), which is soluble in several common organic solvents, including eco-friendly solvents such as ethanol and can be regenerated to cellulose by a treatment with vapors or solutions of hydrochloric acid (Rolland 1993 ; Kontturi et al 2003b ; Kontturi and Lankinen 2010 ). While such thin films have been widely employed to study and to understand the interaction of a variety of biomolecules with cellulose, micro- and macropatterned cellulose films have been shown to be promising materials for the fabrication of protein microarrays, high protein affinity matrices or for sensitive DNA detection (Löscher et al 1998 ; Orelma et al 2011 , 2012 ; Mohan et al 2013b , c ). Blends on the basis of TMSC and other polymers such as styrene or lignins can lead to the formation of micro- and nanostructures as well due to phase separation (Nyfors et al 2009 ; Hoeger et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Photolithographic patterning of cellulose: a versatile dual-tone photoresist for advanced applications

et al. 2014

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In many areas of science and technology, patterned films and surfaces play a key role in engineering and development of advanced materials. Here, we present a versatile toolbox that provides an easy patterning method for cellulose thin films by means of photolithography and enzymatic digestion. A patterned UV-illumination of trimethylsilyl cellulose thin films containing small amounts of a photo acid generator leads to a desilylation reaction and thus to the formation of cellulose in the irradiated areas. Depending on the conditions of development, either negative and positive type cellulose structures can be obtained, offering lateral resolutions down to the single-digit micro meter range by means of contact photolithography. In order to highlight the potential of this material for advanced patterning techniques, cellulose structures with sub-µm resolution are fabricated by means of two-photon absorption lithography. Moreover, these photochemically structured cellulose thin films are successfully implemented as dielectric layers in prototype organic thin film transistors. Such photopatternable dielectric layers are crucial for the realization of electrical interconnects for demanding organic device architectures.Electronic supplementary materialThe online version of this article (doi:10.1007/s10570-014-0471-4) contains supplementary material, which is available to authorized users.

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“…As mentioned above, the positively charged substrate was prepared by adsorption of TMC onto cellulose thin films. Modification of cellulose substrates with TMC as an approach to control nonspecific protein adsorption behavior (using BSA) was already reported earlier [22,23] and the appropriate adsorption conditions for preparation of the cationic TMC substrates were adopted from these studies. In this work, TMC adsorption was monitored by multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and zeta potential measurements (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Lectins at Interfaces—An Atomic Force Microscopy and Multi-Parameter-Surface Plasmon Resonance Study

et al. 2018

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Lectins are a diverse class of carbohydrate binding proteins with pivotal roles in cell communication and signaling in many (patho)physiologic processes in the human body, making them promising targets in drug development, for instance, in cancer or infectious diseases. Other applications of lectins employ their ability to recognize specific glycan epitopes in biosensors and glycan microarrays. While a lot of research has focused on lectin interaction with specific carbohydrates, the interaction potential of lectins with different types of surfaces has not been addressed extensively. Here, we screen the interaction of two specific plant lectins, Concanavalin A and Ulex Europaeus Agglutinin-I with different nanoscopic thin films. As a control, the same experiments were performed with Bovine Serum Albumin, a widely used marker for non-specific protein adsorption. In order to test the preferred type of interaction during adsorption, hydrophobic, hydrophilic and charged polymer films were explored, such as polystyrene, cellulose, N,-N,-N-trimethylchitosan chloride and gold, and characterized in terms of wettability, surface free energy, zeta potential and morphology. Atomic force microscopy images of surfaces after protein adsorption correlated very well with the observed mass of adsorbed protein. Surface plasmon resonance spectroscopy studies revealed low adsorbed amounts and slow kinetics for all of the investigated proteins for hydrophilic surfaces, making those resistant to non-specific interactions. As a consequence, they may serve as favorable supports for biosensors, since the use of blocking agents is not necessary.

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Cationically rendered biopolymer surfaces for high protein affinity support matrices

Cited by 26 publications

References 26 publications

On the formation of Bi 2 S 3 -cellulose nanocomposite films from bismuth xanthates and trimethylsilyl-cellulose

On the formation of Bi 2 S 3 -cellulose nanocomposite films from bismuth xanthates and trimethylsilyl-cellulose

Photolithographic patterning of cellulose: a versatile dual-tone photoresist for advanced applications

Lectins at Interfaces—An Atomic Force Microscopy and Multi-Parameter-Surface Plasmon Resonance Study

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