Cationic Supramolecular Vesicular Aggregates for Pulmonary Tissue Selective Delivery in Anticancer Therapy

Licciardi, Mariano; Paolino, Donatella; Mauro, Nicolò; Cosco, Donato; Giammona, Gaetano; Fresta, Massimo; Cavallaro, Gennara; Celia, Christian

doi:10.1002/cmdc.201600070

Cited by 10 publications

(5 citation statements)

References 65 publications

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“…A549 cells were used because it was demonstrated that a positive charge of a colloidal system could favor its uptake into lung cancer tissue. 28 As shown in Figure 5, the surfactant-free zein nanoparticles exerted a certain cytotoxicity only after 72 h incubation at the greatest protein concentration tested; namely, 100 μg/mL of zein nanoparticles induced a decrease in cell viability of 25%-30% on both the cell lines, thus confirming the potentially safe use of this biomaterial ( Figure 5). …”

Section: Evaluation Of Cytotoxicitymentioning

confidence: 61%

“…28,29 To investigate the cytotoxicity of zein nanoparticles with and without surfactants, MTT-testing was carried out. 25,29 Briefly, cells were plated in 96-well culture dishes (5×10 3 cells/0.2 mL for A549 cells and 10×10 3 cells/ 0.2 mL for K562) and treated with different amounts of nanoparticles (1,10,50, and 100 μg/mL of protein) at various incubation times (24,48, and 72 h).…”

Section: Freeze Drying Of Nanoparticlesmentioning

confidence: 99%

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Sodium deoxycholate-decorated zein nanoparticles for a stable colloidal drug delivery system

Gagliardi¹,

Paolino²,

Iannone³

et al. 2018

IJN

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Background The use of biopolymers is increasing in drug delivery, thanks to the peculiar properties of these compounds such as their biodegradability, availability, and the possibility of modulating their physico-chemical characteristics. In particular, protein-based systems such as albumin are able to interact with many active compounds, modulating their biopharmaceutical properties. Zein is a protein of 20–40 kDa made up of many hydrophobic amino acids, generally regarded as safe (GRAS) and used as a coating material. Methods In this investigation, zein was combined with various surfactants in order to obtain stable nanosystems by means of the nanoprecipitation technique. Specific parameters, eg, temperature, pH value, Turbiscan Stability Index, serum stability, in vitro cytotoxicity and entrapment efficiency of various model compounds were investigated, in order to identify the nanoformulation most useful for a systemic drug delivery application. Results The use of non-ionic and ionic surfactants such as Tween 80, poloxamer 188, and sodium deoxycholate allowed us to obtain nanoparticles characterized by a mean diameter of 100–200 nm when a protein concentration of 2 mg/mL was used. The surface charge was modulated by means of the protein concentration and the nature of the stabilizer. The most suitable nanoparticle formulation to be proposed as a colloidal drug delivery system was obtained using sodium deoxycholate (1.25% w/v) because it was characterized by a narrow size distribution, a good storage stability after freeze-drying and significant feature of retaining lipophilic and hydrophilic compounds. Conclusion The sodium deoxycholate-coated zein nanoparticles are stable biocompatible colloidal carriers to be used as useful drug delivery systems.

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Section: Evaluation Of Cytotoxicitymentioning

confidence: 61%

Section: Freeze Drying Of Nanoparticlesmentioning

confidence: 99%

Sodium deoxycholate-decorated zein nanoparticles for a stable colloidal drug delivery system

Gagliardi¹,

Paolino²,

Iannone³

et al. 2018

IJN

Self Cite

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“…In physiological media, the nanoparticle is covered by plasma proteins leading to surface charge alterations and, concomitantly, changing its biological activity and affinities (Ramos et al, 2017 ). Positive surface charges may facilitate the binding of nanoparticles to cell membranes and might promote unspecific binding to normal tissues, promoting platelet accumulation and hemolytic events (Licciardi et al, 2016 ; Jahan et al, 2017 ; Jurj et al, 2017 ; Jiang et al, 2018 ; Peretz et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

et al. 2018

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Over the years, the scientific importance of nanoparticles for biomedical applications has increased. The high stability and biocompatibility, together with the low toxicity of the nanoparticles developed lead to their use as targeted drug delivery systems, bioimaging systems, and biosensors. The wide range of nanoparticles size, from 10 nm to 1 μm, as well as their optical properties, allow them to be studied using microscopy and spectroscopy techniques. In order to be effectively used, the physicochemical properties of nanoparticle formulations need to be taken into account, namely, particle size, surface charge distribution, surface derivatization and/or loading capacity, and related interactions. These properties need to be optimized considering the final nanoparticle intended biodistribution and target. In this review, we cover light scattering based techniques, namely dynamic light scattering and zeta-potential, used for the physicochemical characterization of nanoparticles. Dynamic light scattering is used to measure nanoparticles size, but also to evaluate their stability over time in suspension, at different pH and temperature conditions. Zeta-potential is used to characterize nanoparticles surface charge, obtaining information about their stability and surface interaction with other molecules. In this review, we focus on nanoparticle characterization and application in infection, cancer and cardiovascular diseases.

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“…It is interesting to notice that the microgels obtained have a suitable diameter to enter cells and tissues, serving as potential drug delivery systems. In addition, they can also serve as pulmonary delivery systems, having dimensions (100–500 nm) suitable to reach bronchioles [ 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Biocompatible and Biodegradable Polyamidoamines Microgels via a Simple and Reliable Statistical Approach

et al. 2022

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Polyamidoamines (PAAs) are biocompatible and biodegradable polymers with a huge potential as biomaterials for pharmaceutical applications. They are obtained by the step-wise aza-Michael polyaddition of bifunctional or multifunctional amines with bisacrylamides in water. To the best of our knowledge, no synthetic protocols leading to hyperbranched PAAs as well as PAA microgels have been published so far. To fill this gap, a statistical approach was established in this work to fine-tune the aza-Michael polyaddition stoichiometry when a multifunctional co-monomer (bf) is added to a mixture of bifunctional monomers with complementary functions (a2 + b2), possibly even in presence of a monofunctional co-monomer (b1), for obtaining either microgels or hyperbranched polymers by a one-pot reaction. For this purpose, two new equations, obtained by reworking the classic Flory–Stockmayer equations, were successfully applied to the synthesis of different model systems, obtaining biocompatible microgels with tunable size distribution (200–500 nm) and properly designed end-chains in a simple and straightforward way. The same mathematical approach allowed us to empirically evaluate the actual number of active reactive functions of the co-monomers. A number of selected systems, being evaluated for their cytotoxicity in vitro, proved highly cytocompatible and, therefore, endowed with great potential for pharmaceutical and medical applications.

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Cationic Supramolecular Vesicular Aggregates for Pulmonary Tissue Selective Delivery in Anticancer Therapy

Cited by 10 publications

References 65 publications

Sodium deoxycholate-decorated zein nanoparticles for a stable colloidal drug delivery system

Sodium deoxycholate-decorated zein nanoparticles for a stable colloidal drug delivery system

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

Synthesis of Biocompatible and Biodegradable Polyamidoamines Microgels via a Simple and Reliable Statistical Approach

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