Cationic supported lipid bilayers for antigen presentation

Neisseria lactamicaantigens complexed with a novel cationic adjuvant

“…This aggregation did not occur in the presence of DDA-BF when using small OMV protein concentrations (288 ± 7 nm for 25 μg/mL of protein in 0.1 mM DDA). This result is similar to that observed by Lincopan et al (2007). 48 Animals were immunized with 200 μL of antigen injected into 2 sites (100 μL/site) subcutaneously, which totaled 5 μg of protein/animal.…”

“…This result is similar to that observed by Lincopan et al (2007). 48 Animals were immunized with 200 μL of antigen injected into 2 sites (100 μL/site) subcutaneously, which totaled 5 μg of protein/animal. The animals were immunized according to the scheme showed in Table 2.…”

Evaluation of three experimental bovine viral diarrhea virus killed vaccines adjuvanted with combinations of Quil A cholesterol and dimethyldioctadecylammonium (DDA) bromide

“…When Quil A is combined with cholesterol (QAC) it forms helical, nanometer scale worm-like micelles (Mitra and Dungan 2001). DDA is a cationic, micelle-forming surfactant (Lincopan et al 2007). In this study, three experimental vaccines, adjuvanted with Q-series adjuvants were compared to commercially available vaccines.…”

Assembly of horseradish peroxidase within supported cationic bilayers

“…In our group, we have been using the BF for novel applications such as adsorption of hydrophobic drugs 19,32,33 and presentation of antigens to the immune system. 23,27 Previous studies on the interaction between such BF and oppositely charged PSS microspheres revealed that, over a range of low salt concentrations (0.5-5 mM NaCl), there was fusion of adjacent BF around the microsphere with production of a continuous bilayer coating. 20 However, it was also previously described that the DODAB bilayer exhibited hydrophobic defects (exposure of hydrophobic regions of the bilayer to the water phase) in the presence of NaCl 20 so that the hydrophobic effect could indeed drive HRP to integration with the bilayer core.…”

“…In this sequence, 0.75 mL of the stock PSS dispersion, 0.03 mL of a 1 mM DODAB BF dispersion and 2.220 mL of a 1 mM NaCl solution were mixed and allowed to interact for 1 h at 25 C yielding final concentrations of 5 Â 10 9 PSS particles/mL and 0.01 mM DODAB, since this last concentration is the one required to cover each particle with a DODAB bilayer. 18,27 In experiments aiming at evaluation of particle concentration effects, the previously quoted proportion between particles and DODAB was maintained, e.g., at 0.5 Â 10 9 PSS particles/mL, 0.03 mL of a 0.1 mM DODAB dispersion was added to produce the cationic biomimetic particles. Particles concentration ranged from 0.5 to 16.6 Â 10 9 particles/mL (see Table 1).…”