Cationic polyelectrolytes: A new look at their possible roles as opsonins, as stimulators of respiratory burst in leukocytes, in bacteriolysis, and as modulators of immune-complex diseases (A review hypothesis)

Ginsburg, Isaac

doi:10.1007/bf00915991

Cited by 51 publications

(69 citation statements)

References 156 publications

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“…On the other hand no significant generation of Oy was observed when LTA-sensitized PMNs were treated with histone alone in the absence of anti-LTA. These results suggest that cationic agents might enhance the interaction of antibodies with antigens present on the surface of ceils (55,56).…”

Section: Effect Of Histone On Superoxide Generation Previous Studiesmentioning

confidence: 80%

“…Our findings that no significant generation of such radicals took place when PMNs had been treated with large amounts of LTA alone suggests that not every agent which binds to PMN membranes elicits a respiratory burst. Thus, LTA differs from other membraneperturbing agents, e.g., lectins (65), FMLP peptides (66,67), saponin (68), digitonin (68), lysolecithin (69), cationic poly-amino acids (49,52,55,56), lipopolysaccharides (58), etc., which bind to PMN and macrophage membranes and elicit a respiratory burst. To secure the generation of O~-and chemiluminescence (CL), LTA-sensitized PMNs must be further treated with anti-LTA antibodies (Figures 1-7).…”

Section: Discussionmentioning

confidence: 99%

“…It appears that the cationic charge upon antibodies might facilitate their interactions with the antigens. Furthermore, cationized antibodies or antigens might persist in the tissues for longer periods (54,55).…”

Section: Discussionmentioning

confidence: 99%

“…laboratory have shown that cationic polyelectrolytes markedly enhance superoxide generation by immune complexes (49,52,(54)(55)(56). It was, therefore, of interest to examine the effect of histone on superoxide generation induced by LTA-anti-LTA complexes (data not shown).…”

Section: Effect Of Histone On Superoxide Generation Previous Studiesmentioning

confidence: 99%

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Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

et al. 1988

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Abstract--Human neutrophils (PMNs) which have been incubated with lipoteichoic acid (LTA) from group A streptococci generated large amounts of superoxide (O~-chemiluminescence and hydrogen peroxide when challenged with anti-LTA antibodies. Cytochalasin B further enhanced Oy generation. The onset of O2 generation by the LTA-anti-LTA complexes was much faster than that induced by BSA-anti-BSA complexes. LTA-treated PMNs generated much less 02 when challenged with BSA complexes, suggesting that LTA might have blocked, nonspecifically, some of the Fe receptors on PMNs. PMNs treated with LTA-anti-LTA complexes further interacted with bystander nonsensitized PMNs resulting in enhanced O~-generation, suggesting that small numbers of LTA-sensitized PMNs might recruit additional PMNs to participate in the generation of toxic oxygen species. Protelolytic enzyme treatment of PMNs further enhanced the generation of 02 by PMNs treated with LTAanti-LTA. Superoxide generation could also be induced when PMNs and anti-LTA antibodies interacted with target cells (fibroblasts, epithelial cells) pretreated with LTA. This effect was also further enhanced by pretreatment of the target cells with proteases. PMNs incubated with LTA released lysosomal enzymes following treatment with anti-LTA antibodies. The amounts of phosphatase, /3-glucoronidase, Nacetylglucosaminidase, mannosidase, and lysozyme release by LTA-anti-LTA complexes were much smaller than those released by antibody or histone-opsonized streptococci, suggesting that opsonized particles are more efficient lysosomal enzyme releasers. However, since the amounts of O~-generated by the LTA complexes equaled those generated by the opsonized particles, it is assumed that the signals for triggering a respiratory burst and lysosomal enzyme secretion might be different.

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Section: Effect Of Histone On Superoxide Generation Previous Studiesmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Histone On Superoxide Generation Previous Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

et al. 1988

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“…This is important since any delay in its administration might allow more and more histone to be released from disintegrating PMNs which will continue to increase endothelial damage. In this respect, calf thymus histone was also shown to act as a potent opsonin [77], an activator of the respiratory burst in PMNs releasing superoxide and peroxide [78] and histone bound to streptococci, also activated T-cells to generated TH1 cytokines [79]. Finally, engulfment (endocytosis) of histone-coated Candida albicans by fibro-sarcoma cells markedly facilitated their metastasis to the lungs of mice, presumably by recruiting PMNs [80].…”

Section: "Miracle Histones": Is It a Breakthrough In Sepsis Research?mentioning

confidence: 99%

Synergistic Aspects to Explain the Pathophysiology of Sepsis and Septic Shock-An Opinion

Koren¹,

Ginsburg²

2015

J Infect Dis Ther

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Polystyrene Microcarriers Coated with a Combination of Adhesion-Promoting Peptides

Varani

Hillegas²

1994

Animal Cell Technology: Basic &Amp; Applied Aspects

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ABSTRACT. Polystyrene culture dishes and polystyrene microcarriers were coated with two different peptides -Pronectin-F and polylysine, alone and in combination. Each of the two peptides alone supported cell attachment but cell spreading occurred only on Pronectin-F. When the two peptides were used in combination, the effect was synergistic. Attachment and spreading occurred at lower concentrations of peptides when they were used in combination than when they were used individually.

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Cationic polyelectrolytes: A new look at their possible roles as opsonins, as stimulators of respiratory burst in leukocytes, in bacteriolysis, and as modulators of immune-complex diseases (A review hypothesis)

Cited by 51 publications

References 156 publications

Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

Synergistic Aspects to Explain the Pathophysiology of Sepsis and Septic Shock-An Opinion

Polystyrene Microcarriers Coated with a Combination of Adhesion-Promoting Peptides

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