Cationic nanoemulsions as nucleic acids delivery systems

Teixeira, Hélder Ferreira; Bruxel, Fernanda; Fraga, Michelle; Schuh, Roselena Silvestri; Zorzi, Giovanni K.; Matte, Úrsula; Fattal, Elias

doi:10.1016/j.ijpharm.2017.10.030

Cited by 55 publications

(35 citation statements)

References 70 publications

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“…After having selected a formulation with optimal physicochemical properties for gene therapy (it is known that surface properties greatly influence the capacity of cationic nanosystems to associate nucleic acids [30,31], and that size affects their cellular entry, transfection efficiency, and in vivo biodistribution [32,33]), association of miRNA to SNs-ST was attempted. A constant amount of miRNA (10 µg) was added over decreasing amounts of SNs-ST, under magnetic stirring.…”

Section: Of 16mentioning

confidence: 99%

Sphingomyelin-Based Nanosystems (SNs) for the Development of Anticancer miRNA Therapeutics

et al. 2020

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Gene replacement therapy with oncosuppressor microRNAs (miRNAs) is a promising alternative to interfere with cancer progression. However, miRNAs are highly inefficient in a biological environment, hampering a successful translation to clinics. Nanotechnology can tackle this drawback by providing delivery systems able to efficiently deliver them to cancer cells. Thus, the objective of this work was to develop biocompatible nanosystems based on sphingomyelin (SM) for the intracellular delivery of miRNAs to colorectal cancer cells. We pursued two different approaches to select the most appropriate composition for miRNA delivery. On the one hand, we prepared sphingomyelin-based nanosystems (SNs) that incorporate the cationic lipid stearylamine (ST) to support the association of miRNA by the establishment of electrostatic interactions (SNs–ST). On the other hand, the cationic surfactant (DOTAP) was used to preform lipidic complexes with miRNA (Lpx), which were further encapsulated into SNs (SNs-Lpx). Restitution of miRNA145 levels after transfection with SNs-Lpx was related to the strongest anticancer effect in terms of tumor proliferation, colony forming, and migration capacity assays. Altogether, our results suggest that SNs have the potential for miRNA delivery to develop innovative anticancer therapies.

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Section: Of 16mentioning

confidence: 99%

Sphingomyelin-Based Nanosystems (SNs) for the Development of Anticancer miRNA Therapeutics

et al. 2020

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“…Cationic lipids are of extraordinary interest for great ability to interact with DNA plasmids and other genetic materials. Although some cationic materials are somewhat toxic and that limits their use in clinical studies, novel designs and subsequent optimization in structure-transfection activity relationships (SAR) have improved their safety and biodegradability properties [62,63]. Cationic lipids are made up of three defined parts: i.…”

Section: Applications Of Niosomes In Gene Deliverymentioning

confidence: 99%

Cationic Niosomes as Non-Viral Vehicles for Nucleic Acids: Challenges and Opportunities in Gene Delivery

et al. 2019

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Cationic niosomes have become important non-viral vehicles for transporting a good number of small drug molecules and macromolecules. Growing interest shown by these colloidal nanoparticles in therapy is determined by their structural similarities to liposomes. Cationic niosomes are usually obtained from the self-assembly of non-ionic surfactant molecules. This process can be governed not only by the nature of such surfactants but also by others factors like the presence of additives, formulation preparation and properties of the encapsulated hydrophobic or hydrophilic molecules. This review is aimed at providing recent information for using cationic niosomes for gene delivery purposes with particular emphasis on improving the transportation of antisense oligonucleotides (ASOs), small interference RNAs (siRNAs), aptamers and plasmids (pDNA).

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“…This is especially manifested in case of cationic geminis, for which size and morphology strongly controlled by structural characteristics, as well as catanionic surfactants demonstrating unique morphological and rheological behavior [27]. These properties are responsible for the versatile functionality of cationic surfactants and their wide application in modern nanotechnologies as modifiers of soft multifunctional nanocontainers (liposomes, nanoemulsions, and solid lipid nanoparticles) and metal nanoparticles improving their stability and functional activity [10,86,87]. In this review, we emphasized the advantages of cationic surfactants as nanocarrier modifiers allowing to (i) increase their colloidal stability; (ii) improve the encapsulation efficacy and loading capacity; (iii) increase the affinity toward both therapeutic loads and cell membranes; (iv) improve the penetrating through biological barriers, to provide conditions for multidrug delivery; and (v) extend markedly the spectrum of their activity due to introducing additional functionality, e.g., antimicrobial properties, gel-like or liquid crystalline behavior, and catalytic effect.…”

Section: Self-assembling Strategies For Construction Of Soft Nanommentioning

confidence: 99%

Cationic Surfactants: Self-Assembly, Structure-Activity Correlation and Their Biological Applications

Zakharova

Pashirova

Doktorovová

et al. 2019

IJMS

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The development of biotechnological protocols based on cationic surfactants is a modern trend focusing on the fabrication of antimicrobial and bioimaging agents, supramolecular catalysts, stabilizers of nanoparticles, and especially drug and gene nanocarriers. The main emphasis given to the design of novel ecologically friendly and biocompatible cationic surfactants makes it possible to avoid the drawbacks of nanoformulations preventing their entry to clinical trials. To solve the problem of toxicity various ways are proposed, including the use of mixed composition with nontoxic nonionic surfactants and/or hydrotropic agents, design of amphiphilic compounds bearing natural or cleavable fragments. Essential advantages of cationic surfactants are the structural diversity of their head groups allowing of chemical modification and introduction of desirable moiety to answer the green chemistry criteria. The latter can be exemplified by the design of novel families of ecological friendly cleavable surfactants, with improved biodegradability, amphiphiles with natural fragments, and geminis with low aggregation threshold. Importantly, the development of amphiphilic nanocarriers for drug delivery allows understanding the correlation between the chemical structure of surfactants, their aggregation behavior, and their functional activity. This review focuses on several aspects related to the synthesis of innovative cationic surfactants and their broad biological applications including antimicrobial activity, solubilization of hydrophobic drugs, complexation with DNA, and catalytic effect toward important biochemical reaction.

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Cationic nanoemulsions as nucleic acids delivery systems

Cited by 55 publications

References 70 publications

Sphingomyelin-Based Nanosystems (SNs) for the Development of Anticancer miRNA Therapeutics

Sphingomyelin-Based Nanosystems (SNs) for the Development of Anticancer miRNA Therapeutics

Cationic Niosomes as Non-Viral Vehicles for Nucleic Acids: Challenges and Opportunities in Gene Delivery

Cationic Surfactants: Self-Assembly, Structure-Activity Correlation and Their Biological Applications

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