Cationic Liposomes Coated with Polyethylene Glycol As Carriers for Oligonucleotides

Meyer, Olivier; Kirpotin, Dmitri B.; Hong, Keelung; Sternberg, Brigitte; Park, John W.; Woodle, Martin C.; Papahadjopoulos, Demetrios

doi:10.1074/jbc.273.25.15621

Cited by 200 publications

(100 citation statements)

References 23 publications

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“…Cationic liposomes are thought to be potential nonviral carriers, however, present formulations are not satisfactory due to their low transfection efficiency and potent cytotoxicity. [6][7][8][9] On the other hand, polycations, such as spermine, 25 polylysine, 26 and polycationic polymers, 27,28 have been used as tools of gene transfer. The main reason for the usage of these polycations is that polycations enable compaction of DNA.…”

Section: Discussionmentioning

confidence: 99%

“…4,5 Therefore, a number of nonviral systems, especially cationic liposomes, have been developed. [6][7][8][9] Cationic liposomes form a complex with anionic DNA molecules and are thought to deliver DNA through endosomes after endocytosis of the complex, 10 although the precise mechanism for gene transfection mediated by cationic liposomes is still unclear. The cationic liposomal system, however, has some disadvantages such as low efficiency of transfection due to DNA degradation in lysosomes and strong cyototoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

et al. 2000

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A novel gene transfer system was developed by using liposomes modified with cetylated polyethylenimine (PEI, MW 600). This polycation liposome, PCL, showed remarkable transfection efficiency as monitored by the expression of the GFP reporter gene. Most conventional cationic liposomes require phosphatidylethanolamine or cholesterol as a component, although PCLs did not. Egg yolk phosphatidylcholine-and dipalmitoylphosphatidylcholine-based PCL were as effective as dioleoylphosphatidylethanolaminebased PCLs for gene transfer. Concerning the cytotoxicity against COS-1 cells and hemolytic activity, the PCL was superior to conventional cationic liposome preparations. Fur-

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

et al. 2000

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“…Stabilization of cationic liposome complexes with plasmid DNA, 9 or oligonucleotides, 20 can be achieved by inclusion of polyethylene glycol-derivatized lipids. Stabilization of complexes by polyethylene glycol coating or by precondensation of plasmid DNA with polyamines can maintain their in vivo gene transfer activity for up to 2 months.…”

Section: Figure 6 Lipoplex-mediated Multiple Systemic Gene Expressionmentioning

confidence: 99%

Multiple systemic expression of human interferon-β in mice can be achieved upon repeated administration of optimized pcTG90-lipoplex

et al. 2000

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The possibility of achieving multiple systemic expression of human interferon-␤ in mice upon repeated intravenous administration of cationic liposome-DNA complex (lipoplex) was investigated. Lipoplexes containing the pentammonio lipid pcTG90 were first optimized by selecting the most efficient ratio of pcTG90 to phosphatidylethanolamine (DOPE) and the N/P ratio of cationic lipid nitrogen to DNA phosphate. Highest levels and reproducibility of gene expression were obtained using pcTG90/DOPE (1:2) liposomes complexed with the IFNB1 gene containing plasmid pTG14169 at a N/P ratio of 10. Following lipoplex administration, an early but transient human interferon-␤ expression in serum was observed. Importantly, repeated systemic

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“…ynthetic vesicles by polyethylene oxide-polybutadiene (PEO-PBD) or polyethylene oxide-polycaprolactone (PEO-PCL) have advantages 1) carriers are so inert that it does not cause inflammation 2) they are so stealthy that they can circulate in vivo for long time, compared to cationic carriers and lipid vesicles [1][2][3][4][5]. Programmable release of encapsulants by controlling the ratio of PEO-PBD/PEO-polyesters would be another advantage [6,7].…”

Section: Introductionmentioning

confidence: 99%

Efficient Nuclear Delivery and Nuclear Body Localization of Antisense Oligo-Nucleotides using Degradable Polymersomes

Kim

Tewari

Pajeroski

et al. 2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

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Delivery of antisense oligonucleotides, AON, presents many of the same challenges as delivery of any nucleic acid: charge, stability, cell uptake, endolysosomal escape, and entry into the nucleus. Here we demonstrate efficient delivery of AON after loading into biodegradable polymer vesicles or 'polymersomes'. We focus on AON delivery to muscle cells in vitro and in vivo because of the emergence of AON in therapeutic strategies directed at muscular dystrophies. To first clarify uptake kinetics without the complications of typical multilayered myotube cultures, we use micro-patterned C2C12 cells and show efficient uptake of AONpolymersomes. The biodegradable polymersomes break down and foster AON escape with the binding of fluorescent-AON into the nuclear bodies. Intramuscular injections of the polymersome-AON into the hind limbs of mdx-dystrophic mice show more efficient nuclear uptake than AON alone and also lead to dystrophin expression in the mdx mice. In sum, these neutral, degradable carriers of AON show promise in vivo. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of the University of Pennsylvania's products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to pubs-permissions@ieee.org. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.

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Cationic Liposomes Coated with Polyethylene Glycol As Carriers for Oligonucleotides

Cited by 200 publications

References 23 publications

Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

Multiple systemic expression of human interferon-β in mice can be achieved upon repeated administration of optimized pcTG90-lipoplex

Efficient Nuclear Delivery and Nuclear Body Localization of Antisense Oligo-Nucleotides using Degradable Polymersomes

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