Cationic Colloidal Gold Staining of Acidic Glycoconjugates in Mouse Paneth Cells.

Ge, Ying; Yang, Ding; Ohmori, Jun; Tsuyama, Shinichiro; Kim, Bong Seon; Kim, Jae Bong; Murata, Fusayoshi

doi:10.1679/aohc.60.133

Cited by 2 publications

(2 citation statements)

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“…The presence of mucin-like glycoproteins within murine Paneth cells had been previously observed (12,22,24) although neither their identity nor their function was ever resolved. We hypothesized that this granule-associated glycoprotein could be Muc2, which is the main constituent of the intestinal mucus layer.…”

Section: Resultsmentioning

confidence: 96%

“…A number of previous studies have noted the presence of mucin-like glycoproteins within murine Paneth cells (12,22,24) based on histological staining with periodic acid-Schiff stain (or other stains) for mucins or large glycoproteins. Despite these observations, no previous research has explored the identity of these glycoproteins or whether they play a role in the function of Paneth cells.…”

Section: Discussionmentioning

confidence: 99%

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The Muc2 mucin coats murine Paneth cell granules and facilitates their content release and dispersion

Ståhl

Tremblay

Montero

et al. 2018

American Journal of Physiology-Gastrointestinal and Liver Physi

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Paneth cells are a key subset of secretory epithelial cells found at the base of small intestinal crypts. Unlike intestinal goblet cells, which secrete the mucin Muc2, Paneth cells are best known for producing an array of antimicrobial factors. We unexpectedly identified Muc2 staining localized around Paneth cell granules. Electron microscopy (EM) confirmed an electron lucent halo around these granules, which was lost in Paneth cells from Muc2-deficient () mice. EM and immunostaining for lysozyme revealed that Muc2 Paneth cells contained larger, more densely packed granules within their cytoplasm, and we detected defects in the transcription of key antimicrobial genes in the ileal tissues of Muc2 mice. Enteroids derived from the small intestine of wild-type and Muc2 mice revealed phenotypic differences in Paneth cells similar to those seen in vivo. Moreover, lysozyme-containing granule release from Muc2 enteroid Paneth cells was shown to be impaired. Surprisingly, Paneth cells within human ileal and duodenal tissues were found to be Muc2 negative. Thus Muc2 plays an important role in murine Paneth cells, suggesting links in function with goblet cells; however human Paneth cells lack Muc2, highlighting that caution should be applied when linking murine to human Paneth cell functions. NEW & NOTEWORTHY We demonstrate for the first time that murine Paneth cell granules possess a halo comprised of the mucin Muc2. The presence of Muc2 exerts an impact on Paneth cell granule size and number and facilitates the release and dispersal of antimicrobials into the mucus layer. Interestingly, despite the importance of Muc2 in murine Paneth cell function, our analysis of Muc2 in human intestinal tissues revealed no trace of Muc2 expression by human Paneth cells.

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Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%