Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis

Tingaud‐Sequeira, Angèle; Carnevali, Oliana; Cerdà, Joan

doi:10.1016/j.cbpa.2010.11.002

Cited by 23 publications

(12 citation statements)

References 67 publications

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“…The yolk syncytial layer (YSL) or periblast consists of a vitellolysis zone and a cytoplasmic zone surrounding the yolk, and is therefore considered to play an integral role in the absorption of yolk in fish [88]. Accordingly, it is known that the YSL express a number of apolipoproteins, cysteine or aspartic proteases (cathepsins), and lipases, which allow yolk protein hydrolysis and lipid mobilization and absorption [89], [90], [91], [92], [93]. In our study, we found that the lipoprotein lipase encoding gene ( lpl ) is rapidly up-regulated at 30 min of aerial exposure which would be consistent with the immediate requirement of lipid mobilization to sustain enhanced embryonic development.…”

Section: Resultsmentioning

confidence: 99%

A Rapid Transcriptome Response Is Associated with Desiccation Resistance in Aerially-Exposed Killifish Embryos

et al. 2013

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Delayed hatching is a form of dormancy evolved in some amphibian and fish embryos to cope with environmental conditions transiently hostile to the survival of hatchlings or larvae. While diapause and cryptobiosis have been extensively studied in several animals, very little is known concerning the molecular mechanisms involved in the sensing and response of fish embryos to environmental cues. Embryos of the euryhaline killifish Fundulus heteroclitus advance dvelopment when exposed to air but hatching is suspended until flooding with seawater. Here, we investigated how transcriptome regulation underpins this adaptive response by examining changes in gene expression profiles of aerially incubated killifish embryos at ∼100% relative humidity, compared to embryos continuously flooded in water. The results confirm that mid-gastrula embryos are able to stimulate development in response to aerial incubation, which is accompanied by the differential expression of at least 806 distinct genes during a 24 h period. Most of these genes (∼70%) appear to be differentially expressed within 3 h of aerial exposure, suggesting a broad and rapid transcriptomic response. This response seems to include an early sensing phase, which overlaps with a tissue remodeling and activation of embryonic development phase involving many regulatory and metabolic pathways. Interestingly, we found fast (0.5–1 h) transcriptional differences in representatives of classical “stress” proteins, such as some molecular chaperones, members of signalling pathways typically involved in the transduction of sensor signals to stress response genes, and oxidative stress-related proteins, similar to that described in other animals undergoing dormancy, diapause or desiccation. To our knowledge, these data represent the first transcriptional profiling of molecular processes associated with desiccation resistance during delayed hatching in non-mammalian vertebrates. The exceptional transcriptomic plasticity observed in killifish embryos provides an important insight as to how the embryos are able to rapidly adapt to non-lethal desiccation conditions.

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Section: Resultsmentioning

confidence: 99%

A Rapid Transcriptome Response Is Associated with Desiccation Resistance in Aerially-Exposed Killifish Embryos

et al. 2013

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“…The expression differences between the two populations might be due to the longer incubation of parthenogenetic eggs [12]. In other species, cathepsin B presents a high expression level at different developmental stages [42,43]; therefore, in parthenogenetic H. longicornis, cathepsin B is more likely to be highly expressed in late embryonic development. The activity of cathepsin B fluctuated and was maintained at a high level throughout embryonic development in the parthenogenetic population, but in the bisexual population, it gradually decreased.…”

Section: Discussionmentioning

confidence: 99%

Investigation of three enzymes and their roles in the embryonic development of parthenogenetic Haemaphysalis longicornis

Qiu

et al. 2020

Parasites Vectors

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Background: The tick Haemaphysalis longicornis exhibits two separate reproductive populations: bisexual and parthenogenetic, which have diploid and triploid karyotypes, respectively. The parthenogenetic population can undergo engorgement without copulation and produce viable female-only offspring with a longer incubation period than the bisexual population. Three enzymes, cathepsin B, cathepsin D and acid phosphatase, were found to be involved in vitellin degradation during the embryonic development of bisexual H. longicornis. However, the expression and activity profiles of these enzymes during the embryonic development of parthenogenetic ticks remain unknown. In the present study, the transcriptional expression profile, enzyme activity and roles in embryogenesis of the three enzymes during the embryonic development of parthenogenetic H. longicornis were investigated.Methods: Quantitative real-time polymerase chain reaction (qPCR) and fluorescence detection were used to analyze the dynamic changes in the three enzymes during embryogenesis. The roles of the three enzymes during embryogenesis were also explored using RNA interference (RNAi). Results:The three enzymes were all expressed during embryonic development in parthenogenetic H. longicornis. The expression of cathepsin B was highest on day 15, whereas that of cathepsin D was highest on day 3 and the peak of acid phosphatase expression occurred on day 9. The activity of cathepsin B was highest on day 3 and lowest on day 5, then gradually increased and remained stable. Cathepsin D activity was highest on day 1 and showed a gradually decreasing trend, whereas acid phosphatase showed the opposite trend and reached a peak on day 23. RNA interference experiments in engorged female ticks revealed that there was no significant difference in the number of eggs laid, but the hatching rate of the eggs was significantly decreased. Conclusion:The three enzymes all play important roles in embryonic development of H. longicornis, but the expression patterns and changes in the activity of the enzymes in the bisexual and parthenogenetic populations are different. The results will help a better understanding of the similarities and differences underlying embryonic development in the bisexual and parthenogenetic populations and contribute to the future exploration of the development of the parthenogenetic population of H. longicornis.

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“…Many previous studies have reported that survival of fish embryos during early development could be associated with yolk processing by several proteases ( Carnevali et al, 2001 ; Tingaud-Sequeira et al, 2011 ; Palomino et al, 2017 ). The key enzymes known to be involved in the process are cathepsins and lipoprotein lipase.…”

Section: Introductionmentioning

confidence: 99%

“…In yellowtail kingfish Seriola lalandi , mRNA expressions and enzyme activities of cathepsin B, cathepsin D and cathepsin L were associated with the survival of embryos during the early development ( Palomino et al, 2017 ). In addition, during maturation, the key enzymes such as cathepsin B, D, and L were involved in the egg development and early embryonic development of fish ( Carnevali et al, 1999a , 1999b , 2001 , 2008 ; Kwon et al, 2001 ; Tingaud-Sequeira et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Expression of Yolk Processing Enzyme Genes in Fertilized Eggs from Artificially Matured Female Eel, Anguilla japonica

Kim

Mun

et al. 2018

Dev. Reprod.

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Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica. Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl, respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels. Expressions of yolk processing enzyme genes did not show significant difference between floating and sinking egg groups. Expression of ctsb decreased when fertilization rate was high. Expression of ctsd, ctsl and lpl, however, did not show any significant differences. These results suggest that ctsb expression could be an indicator of egg quality, and that some proteins prone to be digested by ctsb could be very important in the process of fertilization and normal cleavage in this species. Further study should identify these critical proteins to improve our understanding on the quality of fish eggs.

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Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis

Cited by 23 publications

References 67 publications

A Rapid Transcriptome Response Is Associated with Desiccation Resistance in Aerially-Exposed Killifish Embryos

A Rapid Transcriptome Response Is Associated with Desiccation Resistance in Aerially-Exposed Killifish Embryos

Investigation of three enzymes and their roles in the embryonic development of parthenogenetic Haemaphysalis longicornis

Expression of Yolk Processing Enzyme Genes in Fertilized Eggs from Artificially Matured Female Eel, Anguilla japonica

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