Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures

The effects of methyl jasmonate, salicylic acid and ethylene on alkaloid accumulation in in vitro cell suspension, hairy roots and rootless shoot cultures of Catharanthus roseus were analyzed. Ajmalicine, but not catharanthine, accumulation was promoted by jasmonate and ethylene treatments in cell suspensions. In hairy roots, jasmonate induced the accumulation of both alkaloids, whereas ethylene only induced catharanthine accumulation. In shoot cultures, positive effects of jasmonate and ethylene were recorded only in vindoline accumulation. Ethylene diminished catharanthine accumulation in these cultures. No effect of salicylic acid was observed in any of the studied in vitro culture systems.

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“…1a). Ajmalicine and catharanthine were also the main alkaloids in the J1 hairy roots line (Vázquez-Flota et al 1994). Both showed a Fig.…”

Section: Resultsmentioning

confidence: 99%

A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

et al. 2008

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“…The relationship of the trend of FW/DW to substrate depletions was also very striking. Though 2%-6% sucrose is commonly examined for influence of sucrose on output of C. roseus hairy roots (Jung et al, 1992;Parr et al, 1988;Shanks and Bhadra, 1997;Toivonen et al, 1991;Vasquez-Flota et al, 1994), effects of this and other nutrients on biomass are not often quantified by substrate yield coefficients and specific rates from transient data. Jung et al (1992) have reported biomass yield coefficients on glucose, fructose, and sucrose determined by an unspecified method: 0.53 g/g with 3% sucrose and 1/3 Shenk-Hildebrandt nutrients.…”

Section: Discussionmentioning

confidence: 99%

“…Published reports of hairy root cultures of C. roseus have focused largely on ajmalicine, serpentine, and/or catharanthine in a variety of short-and medium-term studies (Ciau-Uitz et al, 1994;Islas et al, 1994;Parr et al, 1988;Toivonen et al, 1989Toivonen et al, , 1990Vasquez-Flota et al, 1994). Though tabersonine is known to occur in hairy roots (Parr et al, 1988;Toivonen et al, 1989), its kinetics of accumulation has not been studied.…”

Section: Introductionmentioning

confidence: 95%

Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots ofCatharanthus roseus

Bhadra

Shanks

1997

Biotechnol. Bioeng.

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The kinetics of growth, the uptake of macronutrients, and the accumulation of indole alkaloids were investigated in long-term, heterotrophically cultured transgenic (''hairy'') roots of Catharanthus roseus. Tabersonine, ajmalicine, and serpentine were monitored over a 70-day period. The doubling time [dry-weight (DW) basis] of C. roseus hairy roots in B5/2 nutrients supplemented with 3% sucrose was 3.6 days. NH 4 + , NO 3 , − and P i were depleted sequentially from culture medium by hairy roots, while sugars remained undepleted. The growth-limiting nutrient was inorganic nitrogen, NH 4 + and NO 3 − , with exponential-phase overall biomass yields of 34.1 and 5.0 g DW/g nutrient, respectively. Extracellular pH decreased to 4.8 in early exponential phase of culture growth from the initially adjusted value of 5.7, increased subsequently to a maximum of 7.7 in late exponential phase of growth coincident with the maximum of fresh weight (FW)/DW ratio, before decreasing to 5.5-5.0. The organic acids, pyruvate, formate, lactate, and succinate were excreted by hairy roots starting in late phase of exponential growth, possibly resulting in the late-culture pH decrease. Tabersonine accumulation was distinctly growth associated with maximum specific and total yields of 1.15 mg/g DW and 5.6 mg/L, respectively, in late-exponential phase of growth. Serpentine accumulation was non-growth associated with increasing specific and total levels in stationary growth phase: 1.3 mg/g DW and 10.5 mg/L, respectively. The accumulation of ajmalicine also appeared growth associated.

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“…It is also observed in case of Catharunthus roseus hairy root cultures where no net change in the alkaloid content was observed with the changes in the concentration of vitamins. 26 Nevertheless, enhancement in secondary metabolite productivity had also been reported earlier through supplementation of the half strength B5 medium formulation with the vitamin composition of the full strength media. 27 In case of Polygonum tinctorium 22 hairy root cultures, lower pH levels influenced the biomass productivities in a similar fashion.…”

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confidence: 81%

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

Verma

Singh

Negi

et al. 2015

Plant Signaling & Behavior

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Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers a potential production system for iridoid glycosides. In present study we have investigated the effects of various nutrient medium formulations viz B5, MS, WP and NN, and sucrose concentrations (1-8%) on the biomass and glycoside production of selected clone (14-P) of P. kurroa hairy root. Full strength B5 medium was found to be most suitable for maximum biomass yield on the 40th day of culture (GI D 32.72 § 0.44) followed by the NN medium of the same strength (GI D 22.9 § 0.43). Secondary metabolite production was 1.1 and 1.3 times higher in half strength B5 medium respectively in comparison to MS medium. Maximum biomass accumulation along with the maximum picroliv content was achieved with 4% sucrose concentration in basal medium. RT vitamin and Thiamine-HCl effected the growth and secondary metabolite production of hairy roots growing on MS medium but did not show any effect on other media. The pH of the medium played significant role in growth and secondary metabolite production and was found to be highest at pH 6.0 while lowest at pH 3.0 and pH 8.0. To enhance the production of biomass and Picroliv 5 liter working capacity bioreactor was used, 27-fold (324 g FW) higher growth was observed in bioreactor than shake flask and secondary metabolite production was similarly enhanced.

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Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures

Cited by 73 publications

References 27 publications

A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots ofCatharanthus roseus

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

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