A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

Vázquez‐Flota, Felipe; Hernández-Domínguez, Elizabeta; Miranda-Ham, Ma. de Lourdes; Monforte‐González, Miriam

doi:10.1007/s10529-008-9881-4

Cited by 53 publications

(30 citation statements)

References 10 publications

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“…In the elicitation process, jasmonates play an important role as regulatory signals to induce de novo transcription and translation leading to the induction of secondary metabolite biosynthesis in plant cell cultures Zhao and Verpoorte 2007). Exogenous application of jasmonates to C. roseus cell cultures (El-Sayed and Verpoorte 2002; Lee-Parsons and Royce 2006;Vázquez-Flota et al 2009), hairy roots (Rijhwani and Shanks 1998;Vázquez-Flota et al 2009), shoot cultures (Vázquez-Flota et al 2009) and seedlings (ElSayed and Verpoorte 2004) increased the production of TIA caused by an elevated expression of a set of biosynthesis related genes . Combination of transcript and metabolic profiling of jasmonate elicited C. roseus cell cultures yielded a collection of known and previously undescribed transcript tags and metabolites associated with TIA (Rischer et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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In Catharanthus roseus cell cultures, the terpenoid moiety is considered as the limiting factor in terpenoid indole alkaloid (TIA) production. The pathway of terpenoidal precursors in TIA is strongly linked with other terpenoid pathways, suggesting that TIA production might be limited by competition for the five-carbon (C5) precursors. This raises the question whether the stimulation of TIA production by certain signal molecules is due to a redistribution of C5 precursors between the associated terpenoid pathways and/or to a total increase of the precursor availability. To investigate the effect of a TIA-increasing signal molecule on C5 distribution, the cell suspension cultures of C. roseus were elicited with jasmonic acid (JA) and the metabolic changes of different terpenoid pathways were evaluated targeting on TIA (monoterpenoid; C10), carotenoids (tetraterpenoid; C40), and phytosterols (triterpenoid; C30). The chromatographic analyses showed that TIA and carotenoid levels almost doubled upon JA elicitation, while phytosterol levels remained constant if compared to the controls. Apparently, both TIA and carotenoid routes benefit from an increased C5 flow in the methyl-erythritol phosphate pathway, and potential export of C5 precursors to the cytosolic terpenoid routes, e.g., towards the phytosterols is minimal. However, the relative composition of individual compounds within each group remains similar when comparing elicited and control cells. This suggests that the increased production of TIA and carotenoid upon JA elicitation is predominantly caused by an increase of the precursor availability rather than due to a redistribution of C5 precursors between the associated terpenoid pathways. Furthermore, NMR-based metabolomics analysis showed a discrimination of JAelicited and control cells between 24 and 72 h after treatments with significant changes in levels of strictosidine, malic acid, and sucrose. This study portrays metabolic alterations upon JA elicitation and channeling of C5 precursors in different terpenoid biosynthetic pathways, which provides a knowledge platform for developing strategies to engineer fluxes in a complex biosynthetic network in order to obtain high TIA-producing C. roseus cell lines.

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Section: Introductionmentioning

confidence: 99%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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“…The production of ajmalicine in cell suspensions, hairy roots, and shoot cultures of C. roseus is greatly enhanced by JA and ethylene but not by salicylic acid. The hormoneinduced increases of the TIAs, in general, are accompanied by an increase of TDC activity (Vázquez-Flota et al, 2009). CrWRKY1 is highly up-regulated by MJ, ethylene, and GA; however, the different induction patterns of the three chemicals suggest that separate induction pathways are involved in governing CrWRKY1 expression.…”

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confidence: 99%

The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

et al. 2011

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Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source of natural or semisynthetic anticancer drugs. The biosynthesis of TIAs is tissue specific and induced by certain phytohormones and fungal elicitors, indicating the involvement of a complex transcriptional control network. However, the transcriptional regulation of the TIA pathway is poorly understood. Here, we describe a C. roseus WRKY transcription factor, CrWRKY1, that is preferentially expressed in roots and induced by the phytohormones jasmonate, gibberellic acid, and ethylene. The overexpression of CrWRKY1 in C. roseus hairy roots up-regulated several key TIA pathway genes, especially Tryptophan Decarboxylase (TDC), as well as the transcriptional repressors ZCT1 (for zinc-finger C. roseus transcription factor 1), ZCT2, and ZCT3. However, CrWRKY1 overexpression repressed the transcriptional activators ORCA2, ORCA3, and CrMYC2. Overexpression of a dominant-repressive form of CrWRKY1, created by fusing the SRDX repressor domain to CrWRKY1, resulted in the downregulation of TDC and ZCTs but the up-regulation of ORCA3 and CrMYC2. CrWRKY1 bound to the W box elements of the TDC promoter in electrophoretic mobility shift, yeast one-hybrid, and C. roseus protoplast assays. Up-regulation of TDC increased TDC activity, tryptamine concentration, and resistance to 4-methyl tryptophan inhibition of CrWRKY1 hairy roots. Compared with control roots, CrWRKY1 hairy roots accumulated up to 3-fold higher levels of serpentine. The preferential expression of CrWRKY1 in roots and its interaction with transcription factors including ORCA3, CrMYC2, and ZCTs may play a key role in determining the root-specific accumulation of serpentine in C. roseus plants.

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“…Differential roles of these compounds in cell responses to the same type of stress have been frequently documented (Li et al 2006). Nevertheless, the participation of SA in modifying secondary metabolism should not be discarded since responses to these stress hormones may depend on the type of exposed tissue (Vázquez-Flota et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Fusarium oxysporum homogenates and jasmonate induce limited sanguinarine accumulation in Argemone mexicana cell cultures

et al. 2010

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In vitro cultures of Argemone mexicana (Papaveraceae) were induced from leaves of mature plants. Sanguinarine, a benzophenanthridine, was the main alkaloid in the cultures, even in the absence of inducers of secondary metabolism. The accumulation of this metabolite was increased by adding methyl jasmonate and fungal elicitors, although in a limited fashion in comparison to other sanguinarine-producing species. Evidence of a transport mechanism, which may be related to the magnitude of the response, was obtained based on the fluorescent properties of bezophenathridines in the elicited cultures.

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A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

Cited by 53 publications

References 10 publications

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

Fusarium oxysporum homogenates and jasmonate induce limited sanguinarine accumulation in Argemone mexicana cell cultures

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