Catechol ring-cleavage in Pseudomonas cepacia: the simultaneous induction of ortho and meta pathways

Hamzah, Riyad Y.; Al-Baharna, B. S.

doi:10.1007/bf00186968

Cited by 65 publications

(37 citation statements)

References 26 publications

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“…This result is also supported by the findings of Gibson et al [16] who attributed yellow coloration during benzene degradation to the formation of 2-HMS. On the other hand, the ortho-cleavage product, cis,cis-muconate, has a characteristic absorbance at 255 nm [17], and no intermediate peaks were formed near this wavelength. The apparently slow rate of catechol degradation was likely a result of the limitation of oxygen in the cuvette, and is not representative of the conditions in a bioreactor or bioscrubber.…”

Section: Formation Of Intermediate Metabolitesmentioning

confidence: 98%

Benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation

Nielsen

Sask

McLellan

2006

Bioprocess Biosyst Eng

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The performance and stability of a two-phase partitioning bioscrubber (TPPB) containing 33% (vol.) n-hexadecane as an immiscible phase was investigated during 30 days of continuous gaseous benzene treatment. Elimination capacities of 141 +/- 12 g/m(3) h were achieved by Achromobacter xylosoxidans Y234 while maintaining >99% removal throughout. A new steady-state operating strategy that limits excessive biomass production by directing substrate consumption to maintenance energy has eliminated the requirement for frequent exchange of liquid contents. Simplifying the operating protocols in this manner has dramatically reduced material costs and rendered the TPPB operational requirements as more comparable (in terms of frequency of required operator inputs) with other vapor-phase bioreactors. The practicality of the proposed simplification to the operating protocol was confirmed by demonstrating that intermediate metabolites were not accumulating in the TPPB, inorganic nutrient requirements were readily predictable, and that high culture viability could be sustained for prolonged cell retention times (30 days).

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Section: Formation Of Intermediate Metabolitesmentioning

confidence: 98%

Benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation

Nielsen

Sask

McLellan

2006

Bioprocess Biosyst Eng

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“…There is toxicological concern about the principal processes for the removal of PAHs from the presence of PAHs in the environment as they have been environment are thought to be microbial transformation and found to exhibit toxic, mutagenic and carcinogenic properties degradation (Gibson et al 1975). Low molecular weight (Andrews et al 1978;Mersch-Sundermann et al 1992; PAHs, such as naphthalene, are known to have short half- Nylund et al 1992;Fujikawa et al 1993). Numerous studies lives whereas high molecular weight PAHs, such as have shown that one-, two-and three-ring compounds are benzo [a]pyrene, are considered to be highly recalcitrant and acutely toxic (Sims and Overcash 1983) and high molecular may persist in the environment indefinitely (Cerniglia 1992).…”

Section: Merschmentioning

confidence: 99%

“…Similar behaviour has been observed with a soil isolate collected from a coal gasification plant (Grosser et al 1991) and the prior exposure of marine sediments to naphthalene or phenanthrene has been observed to stimulate the degradation rate of phenanthrene and anthracene, respectively (Bauer and Capone 1988). It is known that some enzymes involved in PAH degradation are (Hamzah and Al-Baharna 1994 …”

Section: Co-metabolism Of High Molecular Weight Pahsmentioning

confidence: 99%

Degradation of fluoranthene, pyrene, benz[ a ]anthracene and dibenz[ a , h ]anthracene by Burkholderia cepacia

Juhasz

Britz

Stanley

1997

Journal of Applied Microbiology

145

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Microbiological analysis of soils from a polycyclic aromatic hydrocarbon (PAH)‐contaminated site resulted in the enrichment of five microbial communities capable of utilizing pyrene as a sole carbon and energy source. Communities 4 and 5 rapidly degraded a number of different PAH compounds. Three pure cultures were isolated from community 5 using a spray plate method with pyrene as the sole carbon source. The cultures were identified as strains of Burkholderia (Pseudomonas) cepacia on the basis of biochemical and growth tests. The pure cultures (VUN 10 001, VUN 10 002 and VUN 10 003) were capable of degrading fluorene, phenanthrene and pyrene (100 mg l−1) to undetectable levels within 7–10 d in standard serum bottle cultures. Pyrene degradation was observed at concentrations up to 1000 mg l−1. The three isolates were also able to degrade other PAHs including fluoranthene, benz[a]anthracene and dibenz[a,h]anthracene as sole carbon and energy sources. Stimulation of dibenz[a,h]anthracene and benzo[a]pyrene degradation was achieved by the addition of small quantities of phenanthrene to cultures containing these compounds. Substrate utilization tests revealed that these micro‐organisms could also grow on n‐alkanes, chlorinated‐ and nitro‐aromatic compounds.

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“…Due to the environmental concerns related to these hazardous aromatic compounds, various ways to eliminate or reduce their environmental presence have been pursued including bioremediation using soil microorganisms [7,8]. It has been well documented that certain Gram-negative soil bacteria, including Pseudomonas or Acinetobacter species, are capable of completely degrading relatively simple aromatic compounds including phenol and benzoate [8,9]. Furthermore, the enzymes and the genes involved in aromatic compound biodegradation have also been intensively elucidated at both biochemical and molecular genetic levels [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…In general, most aromatic compounds are aerobically degraded through a common intermediate, catechol or protocatechuate depending on the chemical structure of the starting compound [10]. The catechol is further degraded either by cleavage between two hydroxyl groups by catechol 1,2-dioxygenase (C12O) via an ortho-pathway or by cleavage adjacent to the hydroxyl groups by catechol 2,3-dioxygenase via a meta-pathway [9,10], respectively. Recently, some Gram-positive soil bacteria including the Arthrobacter and Rhodococcus species were also isolated from various environments and characterized as containing a similar C12O-dependent catechol-degrading orthopathway like the one found in the Gram-negative bacteria [12^16].…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of thermophilic catechol 1,2-dioxygenase gene (catA) from Streptomyces setonii

2001

FEMS Microbiology Letters

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Streptomyces setonii (ATCC 39116) degrades various single aromatic compounds such as phenol or benzoate via an ortho-cleavage pathway using catechol 1,2-dioxygenase (C12O). A PCR using degenerate primers based on the conserved regions of known C12O-encoding genes amplified a 0.45-kbp DNA fragment from S. setonii total DNA. A Southern hybridization analysis and size-selected DNA library screening using the 0.45-kbp PCR product as a probe led to the isolation of a 6.4-kbp S. setonii DNA fragment, from which the C12O-encoding genetic locus was found to be located within a 1.4-kbp DNA fragment. A complete nucleotide sequencing analysis of the 1.4-kbp DNA fragment revealed a 0.84-kbp open reading frame, which showed a strong overall amino acid similarity to the known high-G+C Gram-positive (but significantly less to the Gram-negative) bacterial mesophilic C12Os. The heterologous expression of the cloned 1.4-kbp DNA fragment in Escherichia coli demonstrated that this C12O possessed a thermophilic activity within a broad temperature range (up to 65³C) and showed a higher activity against 3-methylcatechol than catechol or 4-methylcatechol, but no activity against protocatechuate. ß

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Catechol ring-cleavage in Pseudomonas cepacia: the simultaneous induction of ortho and meta pathways

Cited by 65 publications

References 26 publications

Benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation

Benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation

Degradation of fluoranthene, pyrene, benz[ a ]anthracene and dibenz[ a , h ]anthracene by Burkholderia cepacia

Cloning and expression of thermophilic catechol 1,2-dioxygenase gene (catA) from Streptomyces setonii

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